Effect of human mesenchymal stem cells expressing secreted protein IFN-β/EGFP on proliferation in prostate cancer cells line PC-3

詹以安; 王共先; 傅斌

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Effect of human mesenchymal stem cells expressing secreted protein IFN-β/EGFP on proliferation in prostate cancer cells line PC-3

更新时间：2026-01-09

- Effect of human mesenchymal stem cells expressing secreted protein IFN-β/EGFP on proliferation in prostate cancer cells line PC-3
- Journal of Army Medical University Vol. 34, Issue 8, Pages: 761-764(2012)
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  CLC： R737.25;R394-33;R341
- Published：2012
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詹以安,王共先,傅斌.骨髓间充质干细胞表达分泌型蛋白IFN-β/EGFP对前列腺癌PC-3细胞增殖的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(08):761-764.

　Zhan Yian,Wang Gongxian,Fu Bin.Effect of human mesenchymal stem cells expressing secreted protein IFN-β/EGFP on proliferation in prostate cancer cells line PC-3[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(08):761-764.
詹以安,王共先,傅斌.骨髓间充质干细胞表达分泌型蛋白IFN-β/EGFP对前列腺癌PC-3细胞增殖的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(08):761-764. DOI：

　Zhan Yian,Wang Gongxian,Fu Bin.Effect of human mesenchymal stem cells expressing secreted protein IFN-β/EGFP on proliferation in prostate cancer cells line PC-3[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(08):761-764. DOI：

摘要

目的 β干扰素(interferon-beta

IFN-β)/增强型绿色荧光蛋白（enhanced green fluorescent protein

EGFP）真核质粒转染人骨髓间充质干细胞(human mesenchymal stem cells

hMSCs)，观察转染后的hMSCs（IFN-β-hMSCs）对前列腺癌细胞株PC-3增殖情况的影响。方法构建IFN-β/EGFP分泌型真核表达质粒pEGFP-N2-IFN-β，脂质体介导下转染hMSCs，使用荧光显微镜检测IFN-β/EGFP蛋白中绿色荧光的表达；ELISA法检测细胞上清中IFN-β的表达水平。将IFN-β-hMSCs与PC-3在Transwell培养体系中共培养，同时设立hMSCs共培养组、空质粒转染hMSCs共培养组、PC-3单独培养组为对照组，分别于共培养后第1、3、5天对PC-3进行台盼蓝染色，计数存活细胞数目。结果荧光显微镜下观察到IFN-β-hMSCs显示出绿色荧光，ELISA检测证实IFN-β-hMSCs的细胞上清中存在IFN-β的分泌表达。实验组PC-3细胞增殖受抑制，实验组与各对照组之间比较差异存在统计学意义(P0.05)。结论分泌型蛋白IFN-β/EGFP在hMSCs中可获得表达且保持其各自的生物学特性，在Transwell共培养中可以有效抑制前列腺癌细胞增殖。

Abstract

Objective To determine the effect of interferon-β (IFN-β)/ enhanced green fluorescent protein (EGFP)-expressing mesenchymal stem cells (IFN-β-hMSCs) on the proliferation of prostate cancer cell line PC-3. Methods The secreted eukaryotic expressed vector for recombinant secreted protein IFN-β/EGFP was constructed and then transfected into hMSCs by lipofectamine. EGFP was detected by inverted fluorescence microscopy. The expression of IFN-β in the medium of hMSCs-IFN-β was detected by ELISA. IFN-β-hMSCs were incubated with prostate cancer cell line PC-3 in Transwell co-culture system. hMSCs

mock-hMSCs and PC-3 served as control cells. PC-3 cells were trypsinized and viable cells were counted respectively in 1

and 5 d using a hemocytometer after trypan blue staining. Results Fluorescence microscopy showed that the cells had positive fluorescent signals

and IFN-β was detected in the culture medium of IFN-β-hMSCs. IFN-β-hMSCs resulted in a significant decrease in PC-3 cells survival as compared with control groups (P0.05). Conclusion hMSCs expresses secreted protein IFN-β/EGFP successfully after gene transfection

and IFN-β-hMSCs cells inhibit growth significantly in the prostate cancer cells. IFN-β and EGFP keep their individual characteristics with no interference with each other.

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