TGF-β<sub>1</sub> promotes endothelial cells adhesion to smooth muscle cells

贾后军; 王子卫

您当前的位置：

首页 >

文章列表页 >

TGF-β₁ promotes endothelial cells adhesion to smooth muscle cells

更新时间：2026-01-09

- TGF-β₁ promotes endothelial cells adhesion to smooth muscle cells
- Journal of Army Medical University Vol. 34, Issue 11, Pages: 1087-1089(2012)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R322.12;R329-33;R394.3
- Published：2012
- 稿件说明：
移动端阅览
贾后军,王子卫.转化生长因子β1基因促进人平滑肌细胞与内皮细胞黏附[J].陆军军医大学学报(原第三军医大学学报),2012,34(11):1087-1089.

　Jia Houjun,Wang Ziwei.TGF-β1 promotes endothelial cells adhesion to smooth muscle cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(11):1087-1089.
贾后军,王子卫.转化生长因子β1基因促进人平滑肌细胞与内皮细胞黏附[J].陆军军医大学学报(原第三军医大学学报),2012,34(11):1087-1089. DOI：

　Jia Houjun,Wang Ziwei.TGF-β1 promotes endothelial cells adhesion to smooth muscle cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(11):1087-1089. DOI：

摘要

目的探讨TGF-β

基因作用于平滑肌细胞从而促进内皮细胞黏附的作用。方法用单价阳离子脂质体转染试剂DOTAP转染pMAMneo TGF-β

于原代培养的平滑肌细胞，经G418筛选。检测转染pMAMneo TGF-β

和转染pMAMneo 的平滑肌细胞黏附内皮细胞的情况。结果经G418筛选，转染pMAMneo TGF-β

组转化生长因子TGF-β

基因在平滑肌细胞得到有效表达，内皮黏附实验表明转染pMAMneo TGF-β

组平滑肌细胞表面有大量内皮细胞黏附[（32±2）/高倍视野

]

，而对照组平滑肌细胞仅有少量内皮细胞黏附[（11±1）/高倍视野

]

，其与转染pMAMneo组相比，差异显著（P0.01）。结论 TGF-β

基因能有效作用于血管平滑肌细胞从而促进内皮细胞黏附，在血管再生中具有重要作用。

Abstract

Objective To examine the effect of transforming growth factor β

(TGF-β

) gene on promoting endothelial cells adhesion to smooth muscle cells. Methods Smooth muscle cells were transfected with pMAMneo TGF-β

(TGF-β

group) and pMAMneo (control group) by DOTAP liposome transfection reagent

respectively. The positive cell clones were selected with G418

and the expression of TGF-β

was detected by fluorescence analysis. The number of endothelial cells adhering to the transfected smooth muscle cells were determined by microscopy. Results TGF-β

gene was successfully expressed in the smooth muscle cells transfected with pMAMneo TGF-β

. A large number of endothelial cells adhered to the surfaces of the smooth muscle cells of the TGF-β

group

which was significantly greater than that of the control group [(32±2) vs (11±1)

P0.01

]

. Conclusion TGF-β

gene can induce endothelial cells adhesion to smooth muscle cells

which is helpful for vascular tissue engineering.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Mechanism of progesterone promoting uterine smooth muscle contraction through RhoA/ROCK signaling pathway

Deltex-1 overexpression suppresses proliferation and promotes differentiation of bone marrow mesenchymal stem cells into smooth muscle cells in vitro

Peroxisome proliferators-activated receptor-gamma agonist inhibits angiotensin Ⅱ-induced phenotypic switching of vascular smooth muscle cells

Bone marrow-derived mesenchymal stem cells improve acontractile detrusor in rat model

Construction of eukaryotic vector expressing Jagged1 gene and its effect on prolife-ration and apoptosis of vascular smooth muscle cells

Related Author

常青

王丹

陈诚

严小丽

徐惠成

刘禄斌

王延洲

王荥

Related Institution

No data

⁰

TGF-β1 promotes endothelial cells adhesion to smooth muscle cells

DOI：

摘要

Abstract

关键词

Keywords

references

TGF-β₁ promotes endothelial cells adhesion to smooth muscle cells