As<sub>2</sub>O<sub>3</sub> demethylates RASSFIA gene in DU145 prostate cancer cells

倪渐凤; 孙晓娟; 张伟杰; 赵培荣; 王留兴

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As₂O₃ demethylates RASSFIA gene in DU145 prostate cancer cells

更新时间：2026-01-09

- As₂O₃ demethylates RASSFIA gene in DU145 prostate cancer cells
- Journal of Army Medical University Vol. 34, Issue 7, Pages: 639-642(2012)
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  CLC： R737.25;R966;R979.19
- Published：2012
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倪渐凤,孙晓娟,张伟杰,赵培荣,王留兴.三氧化二砷对前列腺癌DU-145细胞RASSF1A基因的去甲基化作用[J].陆军军医大学学报(原第三军医大学学报),2012,34(07):639-642.

　Ni Jianfeng,Sun Xiaojuan,Zhang Weijie,Zhao Peirong,Wang Liuxing.As2O3 demethylates RASSFIA gene in DU145 prostate cancer cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(07):639-642.
倪渐凤,孙晓娟,张伟杰,赵培荣,王留兴.三氧化二砷对前列腺癌DU-145细胞RASSF1A基因的去甲基化作用[J].陆军军医大学学报(原第三军医大学学报),2012,34(07):639-642. DOI：

　Ni Jianfeng,Sun Xiaojuan,Zhang Weijie,Zhao Peirong,Wang Liuxing.As2O3 demethylates RASSFIA gene in DU145 prostate cancer cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(07):639-642. DOI：

摘要

目的研究三氧化二砷（arsenic trioxide

）对激素非依赖性前列腺癌DU145细胞的生长抑制作用及对RASSF1A基因去甲基化和蛋白表达的影响。方法应用MTT法检测不同浓度(0.5、1.0、2.0、4.0、6.0、12.0、20.0 μmol/L)的As

不同作用时间（24、48、72 h）对DU145细胞的生长抑制作用；应用甲基化特异性PCR(MSP)和Western blot检测As

对DU145细胞RASSF1A基因甲基化状态及蛋白表达的影响。结果 As

可抑制DU145细胞的增殖，在一定范围内随着药物浓度的增高，抑制作用逐渐增强(F=838.089

P0.05）；同一浓度作用时间越长，抑制率越高（F=8.849

P0.05）；且As

可使RASSF1A基因甲基化逆转，蛋白重新表达。结论 As

可以逆转前列腺癌DU145细胞RASSF1A基因启动子CpG岛的异常甲基化，诱导该抑癌基因的重新表达，抑制前列腺癌DU145细胞的增殖。

Abstract

Objective To determine the inhibitory effects of arsenic trioxide (As

) on the proliferation in hormonal independent prostate cancer DU145 cells

and on demethylation and expression of Ras-assciation domain family 1A (RASSF1A). Methods MTT assay was used to test the growth of DU145 cells after the treatment of As

at 0.5

1.0

2.0

4.0

6.0

12.0 or 20.0 μmol/L for 24

48 or 72 h. Methylation-specific PCR (MSP) a

nd Western blot analysis were used to detect the methylation status and protein expression of RASSF1A gene in Du145 cells after treatment. Results As

significantly inhibited the proliferation of DU145 cells in a dose- and time-dependent manner (F=838.089

P0.05

F=8.849

P0.05). As

treatment reversed the methylation status of RASSF1A gene and made the protein re-expression. Conclusion As

reverses abnormal methylation status of promoter CpG island of RASSF1A gene in DU145 prostate cancer cells

induces protein re-expression and inhibits the cells proliferation.

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As2O3 demethylates RASSFIA gene in DU145 prostate cancer cells

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摘要

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As₂O₃ demethylates RASSFIA gene in DU145 prostate cancer cells