Adenine translocase 1 gene transfection induces apoptosis of vascular smooth muscle cells in rats with carotid balloon injury

杨易; 李燕; 王霄; 刘洁; 卢巍; 宋耀明

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Adenine translocase 1 gene transfection induces apoptosis of vascular smooth muscle cells in rats with carotid balloon injury

更新时间：2026-01-09

- Adenine translocase 1 gene transfection induces apoptosis of vascular smooth muscle cells in rats with carotid balloon injury
- Journal of Army Medical University Vol. 34, Issue 8, Pages: 738-743(2012)
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  CLC： R322.12;R394.2;R543.402
- Published：2012
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杨易,李燕,王霄,刘洁,卢巍,宋耀明.转染ANT1基因诱导颈动脉球囊损伤大鼠血管平滑肌细胞凋亡[J].陆军军医大学学报(原第三军医大学学报),2012,34(08):738-743.

　Yang Yi,Li Yan,Wang Xiao,Liu Jie,Lu Wei,Song Yaoming.Adenine translocase 1 gene transfection induces apoptosis of vascular smooth muscle cells in rats with carotid balloon injury[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(08):738-743.
杨易,李燕,王霄,刘洁,卢巍,宋耀明.转染ANT1基因诱导颈动脉球囊损伤大鼠血管平滑肌细胞凋亡[J].陆军军医大学学报(原第三军医大学学报),2012,34(08):738-743. DOI：

　Yang Yi,Li Yan,Wang Xiao,Liu Jie,Lu Wei,Song Yaoming.Adenine translocase 1 gene transfection induces apoptosis of vascular smooth muscle cells in rats with carotid balloon injury[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(08):738-743. DOI：

摘要

目的通过腺病毒载体在体转染大鼠颈总动脉球囊损伤模型，研究过表达腺嘌呤核苷酸转位酶-1（adenine nucleotide translocase 1，ANT1）对血管平滑肌细胞凋亡的影响。方法将72只雄性SD大鼠随机分为正常组、单纯损伤组、Ad-GFP转染组、Ad-ANT1转染组，每组18只。用携带ANT1的腺病毒载体（Ad-ANT1）或空载体腺病毒载体（Ad-GFP）局部感染大鼠颈总动脉球囊损伤动物模型，在损伤后7、14、28 d，取大鼠损伤侧颈动脉RT-PCR检测ANT1 mRNA表达，Western blot以及免疫组化检测BAX和Bcl-2表达，TUNEL法原位检测新生内膜及中膜VSMCs凋亡率。结果 Ad-ANT1腺病毒转染后大鼠颈动脉ANT1大量表达，在14 d达到高峰，显著高于Ad-GFP转染组及单纯球囊损伤组（P0.01），至28 d时仍有表达。在7、14 d时，Ad-ANT1转染组BAX阳性表达率显著高于Ad-GFP转染组及单纯损伤组（P0.05）；Bcl-2阳性表达率高于正常血管，但与Ad-GFP转染组和单纯损伤组无显著差异（P0.05）；Ad-ANT1转染组新生内膜及中膜平滑肌细胞凋亡率显著高于其他各组（P0.05）；在14、28 d时，Ad-ANT1转染组新生内膜及中膜面积比（IA/MA）小于Ad-GFP转染组及单纯损伤组（P0.05）。结论腺病毒载体介导过表达ANT1可诱导大鼠颈总动脉球囊损伤动物模型新生内膜及中膜VSMC的凋亡，其作用可能通过上调促凋亡蛋白BAX的表达实现。

Abstract

Objective To study the effect of adenine nucleotide translocase 1 (ANT1) gene over-expression on apoptosis of vascular smooth muscle cells (VSMC) in a rat carotid balloon injury model induced by adenovirus plasmid. Methods Seventy-two SD rats were randomly divided into normal group

non-transfection group

Ad-GFP transfection group and Ad-ANT1 transfection group (18 in each group). ANT1 gene was transfected into rat carotid arteries with Ad-ANT1 adenovirus after a rat carotid balloon injury model was established. The arteries were harvested on days 7

14 and 28 after operation. Expression of ANT-1

BAX

and Bcl-2 in arteries was detected by RT-PCR

Western blotting

and immunohistochemistry

respectively

with HE staining. Apoptosis of VSMC in tunica intima and tunica media of the model was assayed with TUNEL staining. Results The ANT1 gene was significantly expressed in rat carotid arteries after Ad-ANT1 transfection and reached its peak level on day 14

which was significantly higher in Ad-ANT1 transfection group than in Ad-GFP transfection group and balloon injury group（P0.01）. The expression level of BAX was significantly higher in Ad-ANT1 transfection group than in Ad-GFP transfection group and balloon injury group on days 7 and 14 after transfection（P0.05）. The expression level of Bcl-2 was higher in Ad-ANT1 transfection group than in normal group（P0.05）. However

no significant difference was found in expression level of Bcl-2 between the two groups（P0.05）.The apoptosis rate of VSMC in tunica intima and tunica media of the model was significantly higher in Ad-ANT1 transfection group than in the other 3 groups（P0.05）. The tunica intima/ tunica media area ratio in the model was lower in Ad-ANT1 transfection group than in Ad-GFP transfection group and balloon injury group on days 14 and 28 after transfection（P0.05）. Conclusion Adenovirus plasmid-induced over-expression of ANT1 gene induces apoptosis of VSMC in tunica intima/ tunica media of the model by up-regulating the expression of BAX.

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