Differentiation of adipose-derived neural stem cells into GABAergic neurons in vitro

任红苗; 王宜南; 陈继川; 张世昌

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Differentiation of adipose-derived neural stem cells into GABAergic neurons in vitro

更新时间：2026-01-09

- Differentiation of adipose-derived neural stem cells into GABAergic neurons in vitro
- Journal of Army Medical University Vol. 34, Issue 11, Pages: 1067-1070(2012)
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  CLC： R322.8;R329-33;R329.24
- Published：2012
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任红苗,王宜南,陈继川,张世昌.脂肪源性神经干细胞诱导分化为γ-氨基丁酸能神经元的体外研究[J].陆军军医大学学报(原第三军医大学学报),2012,34(11):1067-1070.

　Ren Hongmiao,Wang Yinan,Chen Jichuan,Zhang Shichang.Differentiation of adipose-derived neural stem cells into GABAergic neurons in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(11):1067-1070.
任红苗,王宜南,陈继川,张世昌.脂肪源性神经干细胞诱导分化为γ-氨基丁酸能神经元的体外研究[J].陆军军医大学学报(原第三军医大学学报),2012,34(11):1067-1070. DOI：

　Ren Hongmiao,Wang Yinan,Chen Jichuan,Zhang Shichang.Differentiation of adipose-derived neural stem cells into GABAergic neurons in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(11):1067-1070. DOI：

摘要

目的探讨诱导脂肪源性神经干细胞(neural stem cells

NSCs)分化为GABA能神经元的体外培养方法，为细胞移植修复神经系统损伤提供种子细胞。方法贴壁培养C57BL/6小鼠（附睾脂肪垫）脂肪来源的干细胞（adipose-derived stem cells

ADSCs），流式细胞仪进行表型鉴定（CD45、CD90、CD105）及标志物纤连蛋白（fibronectin）鉴定后，在碱性成纤维细胞生长因子（basic fibroblast growth factor

bFGF）、表皮生长因子（epidermal growth factor

EGF）、2% B27的Neurobasal培养基中诱导成NSCs。用免疫荧光细胞染色法进行NSC特异性标记物Nestin、增殖能力BrdU检测，再次添加脑源性神经营养因子、骨形成蛋白2后，维甲酸（RA）诱导分化为γ-氨基丁酸能神经元

并进行其标志物GABA的检测。结果分离纯化的ADSCs CD90、CD105表达强阳性，CD45表达阴性；诱导后的干细胞球经Nestin及BrdU鉴定为NSCs，且具有较强的增殖能力；神经干细胞诱导分化后的神经元GABA抗体染色呈阳性。结论在特定神经营养因子作用下，ADSCs在体外诱导生成的NSCs可被诱导分化为GABA能神经元。

Abstract

Objective To provide the seed cells for the repair of nervous system after cell transplantation by studying how to induce the differentiation of adipose-derived neural stem cells (NSCs) into GABAergic neurons in vitro. Methods ADSCs

isolated from the epididymal fat pads in C57BL/6 mouse

were adherently cultured. After cell surface antigens (CD45

CD90 and CD105) and ADSCs marker (fibronectin) were identified by flow cytometry

NSCs were induced from neurobasal medium containing basic fibroblast growth factor (bFGF)

epidermal growth factor (EGF) and 2% B27. Nestin

a specific marker of NSC

and its self-proliferation ability (BrdU）were detected with immunofluorescence staining. Differentiation of ADNSC into GABAergic neurons was induced with retinoic acid (RA) by adding neurotrophic factor and bone-formation protein 2. Marker of GABAergic neurons was then detected. Results The CD90

CD105 and fibronectin were positively expressed while the CD45 was negatively expressed in isolated and purified ADSCs. The stem cells

identified as NSCs with nestin and BrdU

showed a potential of self-proliferation ability. The antibody to GABAergic neurons was positively stained. Conclusion ADSCs-derived NSCs in vitro can differentiate into GABAergic neurons under the action of specific nerve growth factors.

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