Effect of serum amyloid P component deficiency on formation of atherosclerosis plaques in mice

李小强; 王尽淘; 桂亚丽; 唐富天; 兰天; 温定文; 吴腾; 王丽京

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Effect of serum amyloid P component deficiency on formation of atherosclerosis plaques in mice

更新时间：2026-01-09

- Effect of serum amyloid P component deficiency on formation of atherosclerosis plaques in mice
- Journal of Army Medical University Vol. 34, Issue 8, Pages: 734-737(2012)
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  CLC： R331.1;R363.25;R543.502
- Published：2012
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李小强,王尽淘,桂亚丽,唐富天,兰天,温定文,吴腾,王丽京.血清淀粉样P蛋白缺失对小鼠动脉粥样硬化斑块形成的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(08):734-737.

　Li Xiaoqiang,Wang Jintao,Gui Yali,Tang Futian,Lan Tian,Wen Dingwen,Wu Teng,Wang Lijing.Effect of serum amyloid P component deficiency on formation of atherosclerosis plaques in mice[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(08):734-737.
李小强,王尽淘,桂亚丽,唐富天,兰天,温定文,吴腾,王丽京.血清淀粉样P蛋白缺失对小鼠动脉粥样硬化斑块形成的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(08):734-737. DOI：

　Li Xiaoqiang,Wang Jintao,Gui Yali,Tang Futian,Lan Tian,Wen Dingwen,Wu Teng,Wang Lijing.Effect of serum amyloid P component deficiency on formation of atherosclerosis plaques in mice[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(08):734-737. DOI：

摘要

目的观察血清淀粉样P蛋白（serum amyloid P component

SAP）缺失对小鼠动脉粥样硬化形成的影响。方法构建载脂蛋白E（Apolipoprotein E）和SAP双基因敲除小鼠（ApoE

-/-

；SAP

-/-

）作为实验组，ApoE

-/-

小鼠作为对照组。2组小鼠各12只，组内雄雌小鼠各半。在小鼠第8周龄时，体质量约20 g，此时开始同时给予2组小鼠高脂饮食，于第16周龄时获取小鼠全血分离得到血清进行血脂分析，处死小鼠后获取其心脏，胸腹主动脉进行油红O染色并进行病理统计。结果双基因敲除小鼠组的高密度脂蛋白胆固醇（HDL-C）明显高于对照小鼠（P0.01），低密度脂蛋白和高密度脂蛋白的比值和动脉粥样硬化指数（AI）小于对照组小鼠且差异显著（P0.01），而总胆固醇（TC），甘油三酯（TG）和低密度脂蛋白胆固醇（LDL-C）浓度无显著性差异（P0.05）；主动脉根部切片和胸腹主动脉的油红O染色结果表明SAP缺失明显减轻了小鼠动脉粥样硬化斑块面积（P0.01）。结论 AP缺失可能通过上调HDL从而抑制ApoE

-/-

小鼠动脉粥样硬化斑块形成。

Abstract

Objective To observe the effect of serum amyloid P component (SAP) deficiency on atherosclerosis（AS）in mice. Methods Apolipoprotein E (ApoE) and SAP knockout mice served as an experimental group (n=12) and ApoE knockout mice served as a control group (n=12). Their average body weight was about 20 g at the age of 8 weeks. The animals were then fed with high fat diet and killed at the age of 16 weeks. Blood

heart

and thoracico-abdominal aorta tissue samples were taken. Serum lipid was analyzed and the AS lesion Plaque area was measured after oil red O staining. Results The HDL-C level was significantly higher while the LDL-C/HDL-C value and atherogenic index（AI）were significantly lower in experimental group than in control group（P0.01）. However

no significant difference was observed in TC

TG and LDL-C levels between the two groups（P0.05）. Oil red O staining showed that SAP deficiency significantly reduced the area of atherosclerosis plaques in heart and thoracico-abdominal aorta tissue samples（P0.01）. Conclusion SAP deficiency inhibits formation of atherosclerosis plaques in mice by up-regulating their HDL-C level.

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