Ultrasound microbubble-mediated heart fatty acid binding protein in treatment of chronic heart failure rats

童欣; 刘地川; 吴泓权; 蔡敏

您当前的位置：

首页 >

文章列表页 >

Ultrasound microbubble-mediated heart fatty acid binding protein in treatment of chronic heart failure rats

更新时间：2026-01-09

- Ultrasound microbubble-mediated heart fatty acid binding protein in treatment of chronic heart failure rats
- Journal of Army Medical University Vol. 34, Issue 5, Pages: 396-400(2012)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R541.610.5;R445.1;R459.9
- Published：2012
- 稿件说明：
移动端阅览
童欣,刘地川,吴泓权,蔡敏.超声微泡介导H-FABP基因改善慢性心衰大鼠的心功能[J].陆军军医大学学报(原第三军医大学学报),2012,34(05):396-400.

　Tong Xin,Liu Dichuan,Wu Hongquan,Cai Min.Ultrasound microbubble-mediated heart fatty acid binding protein in treatment of chronic heart failure rats[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(05):396-400.
童欣,刘地川,吴泓权,蔡敏.超声微泡介导H-FABP基因改善慢性心衰大鼠的心功能[J].陆军军医大学学报(原第三军医大学学报),2012,34(05):396-400. DOI：

　Tong Xin,Liu Dichuan,Wu Hongquan,Cai Min.Ultrasound microbubble-mediated heart fatty acid binding protein in treatment of chronic heart failure rats[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(05):396-400. DOI：

摘要

目的研究超声靶向破坏微泡（ultrasound targeted microbubble destruction，UTMD）技术转染H-FABP基因对心肌梗死后心功能的影响。方法通过结扎成年雄性Wistar大鼠46只，体质量（220±20）g，冠状动脉前降支建立心肌梗死模型。于心肌梗死8周后，将存活的31只心衰大鼠随机分为4组：①H-FABP+超声+微泡组（H-FABP+US/MB组，n=8），用超声破坏携H-FABP基因微泡转染大鼠心肌；②超声+微泡组（US/MB组，n=8），用超声破坏不含基因的微泡；③H-FABP+生理盐水组（H-FABP+NS组，n=8），由颈静脉输入含基因的生理盐水；④单纯手术组（SA组，n=7），仅由颈静脉输入生理盐水；⑤假手术组（SS组，n=6）。基因转染14 d后测定各组大鼠心功能，Western blot检测左心室非梗死区H-FABP表达，ELISA法检测静脉血及非梗死区心肌游离脂肪酸（FFA）含量，硫代巴比妥酸反应物（TBARS）法检测非梗死区心肌组织脂质过氧化水平，免疫组织化学法检测非梗死区心肌诱导型一氧化氮合酶（iNOS）表达。结果心肌梗死大鼠各组与假手术组比较心功能明显下降（P0.05），H-FABP表达明显下降（P0.05），静脉血及心肌FFA含量明显升高（P0.05），丙二醛（MDA）水平明显升高（P0.05），iNOS表达明显升高（P0.05）。H-FABP+超声+微泡组与心肌梗死大鼠中的另外3组比较心功能明显改善（P0.05），H-FABP表达升高（P0.05），心肌FFA含量降低（P0.05），MDA水平降低（P0.05），iNOS表达受抑制（P0.05）。结论超声微泡介导的H-FABP转染可改善大鼠慢性心衰时心功能。可能与通过提高H-FABP表达、降低心肌FFA含量、改善心肌氧化应激水平、进而抑制iNOS的表达有关。

Abstract

Objective To determine the effect of heart fatty acid binding protein (H-FABP) transfected by ultrasound targeted microbubble destruction (UTMD) technology on cardiac function after post-infarction. Methods Myocardial infarction (MI) was induced in Wistar male rats (n=46

220±20 g) by ligating the left anterior descending coronary artery. After 8 weeks

the 31 surviving rats with heart failure were randomly divided into 4 groups

H-FABP+ultrasound+microbubble group (H-FABP+US/MB group

n=8

ultrasound-targeted destruction microbubble loaded with H-FABP gene was transfected to rat heart)

ultrasound+microbubble group (US/MB group

n=8

ultrasonic destruction of microbubbles loaded without gene)

H-FABP+normal saline group (H-FABP+NS group

n=8

normal saline containing gene were infused from jugular vein)

surgery alone group (SA group

n=7

only normal saline were infused from jugular vein)

and sham-operation group (SS group

n=6). In 14 d after transfection

the cardiac function of all the rats were measured

H-FABP expression in non-infarct region of left ventricular was detected by Western blotting

free fatty acid (FFA) content of venous blood and non-infarct region myocardium were detected by ELISA

lipid peroxidation in non-infarct region of left ventricular was detected by thiobarbituric acid reactive substances (TBARS) method

and the iNOS expression in non-infarct region of left ventricular was detected by immunohistochemical assay. Results Compared with sham-operation group

left ventricular ejection fraction (LVEF) and the H-FABP expression were decreased significantly (P0.05)

free fatty acid content and malondialdehyde (MDA) level and the iNOS expression were obviously increased (P0.05) in the MI groups. Compared with US/MB

H-FABP+NS and SA groups

LVEF and the H-FABP expression were apparently increased (P0.05)

free fatty acid content of myocardium and MDA level were obviously decreased (P0.05) and the iNOS expression was markedly inhibited (P0.05) in H-FABP+US/MB group. Conclusion Ultrasound microbubble-mediated H-FABP transfection improves cardiac function via upregulating H-FABP

redu-cing myocardial FFA

ameliorating myocardial oxidative stress and inhibiting iNOS expression.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Efficiency of prostate-targeted homing of bone marrow mesenchymal stem cells in treatment of rat prostatitis mediated by microbubble-enhanced ultrasound

Ultrasound irradiation microbubble verapamil reverses multidrug resistance of neuroblastoma cells in vitro

Comparison of diagnostic efficacy in males with high/intermediate typed anorectal malformations between ultrasound and inverted X-ray exam

Risk prediction model of in-hospital heart failure in patients with coronary heart disease after percutaneous coronary intervention: development and validation

Real-world application of sacubitril/valsartan in patients suffering from heart failure with reduced ejection fraction

Related Author

韩广玮

申雪晴

高文宏

崔栋

尚永刚

刘城城

易善红

陈镜宇

Related Institution

No data

⁰