Cinnamic aldehyde suppresses proliferation and VEGF secretion in human melanoma A375 cells

周凌; 鲁元刚; 杨桂红; 杨涛; 伍津津

您当前的位置：

首页 >

文章列表页 >

Cinnamic aldehyde suppresses proliferation and VEGF secretion in human melanoma A375 cells

更新时间：2026-01-09

- Cinnamic aldehyde suppresses proliferation and VEGF secretion in human melanoma A375 cells
- Journal of Army Medical University Vol. 34, Issue 7, Pages: 602-604(2012)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R730.23;R739.5;R914.4
- Published：2012
- 稿件说明：
移动端阅览
周凌,鲁元刚,杨桂红,杨涛,伍津津.肉桂醛对恶性黑素瘤A375细胞增殖和分泌VEGF的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(07):602-604.

　Zhou Ling,Lu Yuangang,Yang Guihong,Yang Tao,Wu Jinjin.Cinnamic aldehyde suppresses proliferation and VEGF secretion in human melanoma A375 cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(07):602-604.
周凌,鲁元刚,杨桂红,杨涛,伍津津.肉桂醛对恶性黑素瘤A375细胞增殖和分泌VEGF的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(07):602-604. DOI：

　Zhou Ling,Lu Yuangang,Yang Guihong,Yang Tao,Wu Jinjin.Cinnamic aldehyde suppresses proliferation and VEGF secretion in human melanoma A375 cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(07):602-604. DOI：

摘要

目的研究肉桂醛对体外培养的人恶性黑素瘤细胞株A375增殖和分泌血管内皮细胞生长因子（vascular endothelial growth factor， VEGF）的影响。方法 A375细胞在体外培养扩增后，在培养基中分别加入10、20、40 μmol/L肉桂醛，分别在培养24、48、72 h MTT法检测细胞活性。同时收集各个浓度肉桂醛处理不同时相的培养液样本，双抗体夹心ELISA法测定其中VEGF分泌量。结果 10 μmol/L肉桂醛作用24 h即能抑制A375细胞增殖，10、20、40 μmol/L肉桂醛间的抑制强度具有统计学意义（P＜0.01）。40 μmol/L的肉桂醛作用72 h后细胞增殖抑制率最高，达(88.91±4.5)%。随肉桂醛作用时间延长，A375细胞分泌VEGF的量逐渐减少，72 h后各浓度间的抑制作用具有统计学意义（P＜0.01）。40 μmol/L肉桂醛作用72 h后A375细胞分泌的VEGF含量最低，为9.55 pg/ml。结论肉桂醛能抑制人黑素瘤细胞增殖和VEGF的分泌，其抑制强度与药物浓度和作用时间成正比。

Abstract

Objective To investigate the effect of cinnamic aldehyde on the proliferation and secretion of vascular endothelial growth factor (VEGF) in A375 cells. Methods A375 cells were co-cultured with cinnamic aldehyde at the concentrations of 0

20 and 40 μmol/L for 24

48 and 72 h respectively. The proliferation of A375 cells was tested by MTT assay. VEGF level in the supernatant was detected by ELISA. Results Cinnamic aldehyde at 10 μmol/L inhibited A375 cells proliferation after 24 hours’ treatment

Cinnamic aldehyde at 10

20 and 40 μmol/L affected the cell proliferation significantly (P＜0.01). The proliferation of A375 cells was most remarkably inhibited after co-culture of 40 μmol/L cinnamic aldehyde for 72 h

with the highest inhibitory rate of (88.91±4.5)%. VEGF level in the supernatant was reduced gradually with the elapse of time. After the treatment of 72 h

cinnamic aldehyde at all above concentrations resulted in a significant reduction of VEGF secretion (P0.01). The lowest level of VEGF in the A375 cells was 9.55 pg/ml after co-culture of 40 μmol/L cinnamic aldehyde for 72 h. Conclusion Cinnamic aldehyde inhibits the proliferation and decreases VEGF secretion in human melanoma cells in a time- and dose-dependent manner.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Expression of miR-616 in osteosarcoma and its role in proliferation, apoptosis, migration and invasion of tumor cells

Erianin inhibits proliferation of activated T cells by downregulating CyclinB1

Expression of β-arrestin1 in oral squamous cell carcinoma and its effect on cell proliferation, migration and invasion

ORF1p promotes proliferation and invasion of esophageal squamous cell carcinoma cells by regulating AJUBA expression

Hypoxia promotes proliferation of alveolar epithelial cells by regulating chromatin H2A.Z deposition through TAZ-H2A.Z axis

Related Author

FU Wanlei

HAO Xianglin

CAO Ya

XIA Jiying

ZHOU Xiaogang

XU Jiayi

GUO Qiaonan

JING Lin

Related Institution

Department of Pathology， Second Affiliated Hospital， Army Medical University （Third Military Medical University）

Guangxi Key Laboratory of Characteristic Biomedicine， School of Medicine， Guangxi University

Scientific Research Experiment Center， Chengdu Medical College

School of Laboratory Medicine， Chengdu Medical College

School of Medicine， Shihezi University

⁰