Exogenous hydrogen sulfide improves epilepsy-induced cognitive dysfunction in rats

韩晓伟; 段茂海; 陈远寿; 罗孝美; 秦伟; 田虹; 潘贵书; 刘晓红

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Exogenous hydrogen sulfide improves epilepsy-induced cognitive dysfunction in rats

更新时间：2026-01-09

- Exogenous hydrogen sulfide improves epilepsy-induced cognitive dysfunction in rats
- Journal of Army Medical University Vol. 34, Issue 13, Pages: 1269-1272(2012)
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  CLC： R338.6;R749.17;R914.3
- Published：2012
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韩晓伟,段茂海,陈远寿,罗孝美,秦伟,田虹,潘贵书,刘晓红.外源性硫化氢改善癫痫大鼠脑认知功能障碍[J].陆军军医大学学报(原第三军医大学学报),2012,34(13):1269-1272.

　Han Xiaowei,Duan Maohai,Chen Yuanshou,Luo Xiaomei,Qin Wei,Tian Hong,Pan Guishu,Liu Xiaohong.Exogenous hydrogen sulfide improves epilepsy-induced cognitive dysfunction in rats[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(13):1269-1272.
韩晓伟,段茂海,陈远寿,罗孝美,秦伟,田虹,潘贵书,刘晓红.外源性硫化氢改善癫痫大鼠脑认知功能障碍[J].陆军军医大学学报(原第三军医大学学报),2012,34(13):1269-1272. DOI：

　Han Xiaowei,Duan Maohai,Chen Yuanshou,Luo Xiaomei,Qin Wei,Tian Hong,Pan Guishu,Liu Xiaohong.Exogenous hydrogen sulfide improves epilepsy-induced cognitive dysfunction in rats[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(13):1269-1272. DOI：

摘要

目的探讨外源性硫化氢对癫痫(epilepsy，EP)所致大鼠脑认知功能障碍的保护作用及机制。方法利用氯化锂-匹罗卡品腹腔注射复制雄性SD大鼠96只，6周龄，体质量180~220 g。癫痫模型，大鼠分为正常对照组(NC组)、癫痫模型组(EP组)、癫痫+硫氢化钠组（NaHS组）；采用Morris水迷宫检测大鼠的空间学习记忆能力；实时荧光定量PCR检测海马中的胱硫醚-β-合酶（CBS）mRNA 和CREB mRNA表达变化。结果 EP组的空间辨别学习记忆能力与NC组比较明显减退：Morris水迷宫定位航行实验中逃避潜伏期明显延长(P0.01)，第3象限探索有效时间显著缩短 (P0.05)、第3象限游泳距离占总距离的百分比以及穿越平台次数明显减少(P0.01)；NaHS可提高癫痫大鼠的学习记忆能力，与EP组比较，空间学习记忆能力各项检测指标均有显著差异(P0.05，P0.01)。7、30 d时EP组大鼠海马CBS mRNA表达水平明显低于NC组(P0.05)，而NaHS组CBS mRNA表达水平明显高于EP组(P0.05)；EP组海马CREB mRNA表达在7、30 d时均明显低于NC组 (P0.05)，而NaHS组CREB mRNA在24 h、7、30 d时表达显著高于EP组(P0.05)，与NC组相比，7、30 d时均无差异（P0.05）。结论外源性H

S可能通过上调癫痫大鼠其海马CBS、CREB mRNA表达来改善癫痫大鼠的学习记忆能力。

Abstract

Objective To investigate the neuroprotective effect of exogenous hydrogen sulfide (H

S) on cognitive deficits induced by epilepsy in rats. Methods The epilepsy rat model was induced by intraperitoneally administration of LI-PILO. Male SD rats were divided into 3 groups

normal control group (NC group)

epilepsy group (EP group)

EP+NaHS group (NaHS group

intraperitoneally injection of 14 μmol/kg NaHS

in 25 min before PILO administration). The ability of spacial learning and memory of epilepsy rats was tested by Morris water maze. The expression of cystathionine-β-synthase (CBS) and cAMP response element binding protein (CREB) in the hippocampus was observed by real-time PCR in 2 and 24 h

and 7 and 30 d after model establishment. Results Morris water maze test showed that the spatial learning and memory ability of EP rats were decreased when compared with NC group. In EP rats

the escape latency to find the platform was significantly prolonged or extended compared with NC group (P0.01). The times of passing platform

mean exploring time and percentage of swimming distance in original platform quadrant were decreased significantly compared with NC group (P0.01). NaHS improved the ability of learning and memory

with all indices of spatial learning and memory had significant differences when compared with EP group (P0.05

0.01). Compared with NC group

the expression of CBS mRNA in the hippocampus of EP rats were down-regulated (P0.05) in 7 and 30 d. While

the expression of CBS mRNA in NaHS group was higher than EP (P0.05). Compared with NC group

the expression of CREB mRNA of EP rats were down-regulated in chronic period (7 and 30 d

P0.05). The expression of CREB mRNA in NaHS group was higher than EP group at 24 h

and 7 and 30 d (P0.05)

but had no statistical significance at 7 d and 30 d when compared with NC group (P0.05). Conclusion Exogenous H

S exerts neuroprotection effects and improves cognitive deficits in epileptic rats

which may be through upregulating the expression of CBS and CREB mRNA.

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