Extracellular adenosine triphosphate improves growth and invasion of U87 glioma cells

王晖; 冯华; 陈图南; 赵明月; 李博; 李飞

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Extracellular adenosine triphosphate improves growth and invasion of U87 glioma cells

更新时间：2026-01-09

- Extracellular adenosine triphosphate improves growth and invasion of U87 glioma cells
- Journal of Army Medical University Vol. 34, Issue 7, Pages: 589-592(2012)
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  CLC： R730.23;R730.264
- Published：2012
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王晖,冯华,陈图南,赵明月,李博,李飞.细胞外ATP对U87胶质瘤细胞生长和侵袭的作用[J].陆军军医大学学报(原第三军医大学学报),2012,34(07):589-592.

　Wang Hui,Feng Hua,Chen Tunan,Zhao Mingyue,Li Bo,Li Fei.Extracellular adenosine triphosphate improves growth and invasion of U87 glioma cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(07):589-592.
王晖,冯华,陈图南,赵明月,李博,李飞.细胞外ATP对U87胶质瘤细胞生长和侵袭的作用[J].陆军军医大学学报(原第三军医大学学报),2012,34(07):589-592. DOI：

　Wang Hui,Feng Hua,Chen Tunan,Zhao Mingyue,Li Bo,Li Fei.Extracellular adenosine triphosphate improves growth and invasion of U87 glioma cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(07):589-592. DOI：

摘要

目的观察细胞外三磷酸腺苷（adenosine Triphosphate

ATP）对体外培养的U87人脑胶质瘤细胞增殖、侵袭和迁移能力的影响。方法采用MTT法检测不同浓度细胞外ATP对U87胶质瘤细胞增殖的影响；以相同条件下不加ATP作为对照组，以加入100 μmol/L ATP作为处理组，采用Transwell细胞侵袭实验检测100 μmol/L细胞外ATP对U87胶质瘤细胞侵袭能力的影响；用时间显微镜动态观察100 μmol/L 的ATP作用下U87胶质瘤细胞的迁移情况。结果高浓度（5 000 μmol/L）细胞外ATP对U87胶质瘤细胞增殖有显著抑制作用，较低浓度（50、100、500 μmol/L）细胞外ATP对U87胶质瘤细胞增殖无明显影响，5 000 μmol/L ATP组与其他浓度组比较，差异有统计学意义（P0.05）；Transwell侵袭实验中，处理组细胞跨膜细胞数为（163.83±17.81）明显多于对照组（89.83±13.27）（P0.01）；迁移实验中，处理组细胞运动速度为(163.83±17.81)μm/h ，对照组细胞运动速度为(89.83±13.27)μm/h，2组比较，差异有统计学意义（P0.05）。结论 100 μmol/L的细胞外ATP对体外培养的U87胶质瘤细胞的侵袭和迁移具有明显促进作用。

Abstract

Objectives To determine the effect of extracellular adenosine triphosphate (ATP) on the growth and invasion of U87 glioma cells in vitro. Methods Cell counting kit-8 was used to detect the proliferation in U87 cells after the treatment of ATP at different concentrations of 0

100

200

500 and 5 000 μmol/L. Invasion and migration of U87 cells with 100 μmol/L ATP treatment were examined by Transwell and Time microscopy. Results The proliferation of U87 glioma was inhibited obviously by high concentration of ATP (5 000 μmol/L). However

there was no significant impact on the proliferation in U87 cells by a lower amount of extracellular ATP (50

100 and 500 μmol/L). The invasion and migration ability of U87 cells were significantly enhanced by the using of 100 μmol/L ATP. Conclusion Extracellular ATP can significantly enhance the ability of invasion and migration in U87 cells.

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Department of Gastroenterology, Qingdao Central Hospital of Rehabilitation University

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Department of Stomatology， the First Affiliated Hospital of Shihezi University

Department of Pathology， Second Affiliated Hospital， Army Medical University （Third Military Medical University）

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