Expression of FGF-2 and VEGF in prefrontal cortex of chronic unprediectable mild stress rats

佗婷婷; 冯正直; 蒋娟; 涂静; 刘可愚; 杜征

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Expression of FGF-2 and VEGF in prefrontal cortex of chronic unprediectable mild stress rats

更新时间：2026-01-09

- Expression of FGF-2 and VEGF in prefrontal cortex of chronic unprediectable mild stress rats
- Journal of Army Medical University Vol. 34, Issue 13, Pages: 1265-1268(2012)
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  CLC： R322.81;R394.2;R749.41
- Published：2012
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佗婷婷,冯正直,蒋娟,涂静,刘可愚,杜征.FGF-2和VEGF在慢性应激抑郁大鼠前额叶皮层表达[J].陆军军医大学学报(原第三军医大学学报),2012,34(13):1265-1268.

　Tuo Tingting,Feng Zhengzhi,Jiang Juan,Tu Jing,Liu Keyu,Du Zheng.Expression of FGF-2 and VEGF in prefrontal cortex of chronic unprediectable mild stress rats[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(13):1265-1268.
佗婷婷,冯正直,蒋娟,涂静,刘可愚,杜征.FGF-2和VEGF在慢性应激抑郁大鼠前额叶皮层表达[J].陆军军医大学学报(原第三军医大学学报),2012,34(13):1265-1268. DOI：

　Tuo Tingting,Feng Zhengzhi,Jiang Juan,Tu Jing,Liu Keyu,Du Zheng.Expression of FGF-2 and VEGF in prefrontal cortex of chronic unprediectable mild stress rats[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(13):1265-1268. DOI：

摘要

目的探讨慢性应激抑郁大鼠前额叶皮层成纤维细胞生长因子-2 ( fibroblast growth factors-2

FGF-2)和血管内皮生长因子（vascular endothelial growth factor

VEGF）表达变化，分析其在抑郁症病理生理学中的意义。方法 20只体质量180~220 g雄性SD大鼠分为正常对照组（对照组）、慢性应激抑郁模型组（抑郁组），每组10只。利用21 d慢性不可预见性应激刺激（chronic unprediectable mild stress，CUMS）制造抑郁症模型。21 d后，采用敞箱实验(open-field behavior)、糖水消耗实验、摄食量和体质量增量等检测指标评定大鼠行为学改变并检测模型是否成功建立。应用Western blot 法检测FGF-2和VEGF蛋白表达情况；荧光实时定量PCR技术检测目的基因mRNA表达情况。结果 Western blot检测结果显示：抑郁组SD大鼠前额叶皮质层FGF-2(1.23±0.06)、VEGF蛋白表达(1.15±0.27)显著低于对照组( P0.05)；rt-q PCR 结果显示：与对照组相比，抑郁组SD大鼠前额叶皮质层FGF-2 的2

-△△Ct

值为0.47、VEGF的2

-△△Ct

值为0.51

提示二者mRNA表达与对照组相比显著下调( P0.05)。结论 FGF-2与VEGF基因在前额叶中的表达下调可能在抑郁症病理机制中发挥一定作用。

Abstract

Objective To explore the changes of the fibroblast growth factors-2 (FGF-2) and vascular endothelial growth factor (VEGF) in the prefrontal cortex (PFC) of animal model of depression induced by chronic unpredictable mild stress (CUMS)

and to analyze the relationship of FGF-2 and VEGF expression with the etiology and pathophysiology of depression. Methods A total of 20 male Sprague-Dawley rats weighting 180 to 220 g were randomly divided into 2 groups

normal control group and model group

with 10 rats in each group. The depressed model was reduced by CUMS for 21 d. Then the behavior changes of rats were detected through open-field test

sugar consumption test

and body weighting and feeding

to identify the model establishment. The expression of FGF-2 and VEGF at mRNA and protein levels in the PFC were verified by using real-time-PCR and Western blotting. Results The protein expression of FGF-2 (1.23±0.06) and VEGF(1.15±0.27) in PFC were significantly lower in the model group than control group (P0.05). And the mRNA levels of FGF-2 and VEGF in the PFC were also significantly lower in model group than control group (P0.05). Conclusion Down-regulation of FGF-2 and VEGF in the PFC may play certain roles in the pathophysiological process of depression.

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