Transcriptional positive coactivator 4 is involved in senescence of rat bone marrow-derived mesenchymal stem cells

彭毅; 王涛; 粟永萍; 程天民; 史春梦

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Transcriptional positive coactivator 4 is involved in senescence of rat bone marrow-derived mesenchymal stem cells

更新时间：2026-01-09

- Transcriptional positive coactivator 4 is involved in senescence of rat bone marrow-derived mesenchymal stem cells
- Journal of Army Medical University Vol. 34, Issue 7, Pages: 585-588(2012)
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  CLC： R329-33;R329.28;R394.2
- Published：2012
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彭毅,王涛,粟永萍,程天民,史春梦.转录辅助因子PC4参与大鼠骨髓间充质干细胞体外衰老过程的调控[J].陆军军医大学学报(原第三军医大学学报),2012,34(07):585-588.

　Peng Yi,Wang Tao,Su Yongping,Cheng Tianmin,Shi Chunmeng.Transcriptional positive coactivator 4 is involved in senescence of rat bone marrow-derived mesenchymal stem cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(07):585-588.
彭毅,王涛,粟永萍,程天民,史春梦.转录辅助因子PC4参与大鼠骨髓间充质干细胞体外衰老过程的调控[J].陆军军医大学学报(原第三军医大学学报),2012,34(07):585-588. DOI：

　Peng Yi,Wang Tao,Su Yongping,Cheng Tianmin,Shi Chunmeng.Transcriptional positive coactivator 4 is involved in senescence of rat bone marrow-derived mesenchymal stem cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(07):585-588. DOI：

摘要

目的研究转录辅助因子PC4（transcriptional positive coactivator 4）在骨髓间充质干细胞（mesenchymal stem cells

MSCs）体外传代衰老过程中的表达变化特点，初步探讨其在MSCs增殖和衰老中的可能作用。方法通过CCK-8法检测并绘制MSCs生长曲线，流式细胞术检测MSCs免疫表型，通过RT-PCR和Western blot检测PC4在MSCs长期体外培养中的表达特点，并检测细胞衰老标志物β-半乳糖苷酶活性的变化，进一步通过RNA干扰抑制PC4表达，检测其对MSCs增殖和衰老的影响。结果本实验分离培养的MSCs早期具有成体干细胞特性，长期体外连续传代后出现细胞衰老，β-半乳糖苷酶染色呈阳性，PC4在衰老细胞中的表达明显降低，RNA干扰抑制PC4表达后，MSCs增殖受到抑制，β-半乳糖苷酶染色阳性率增加［转染后48 h阴性对照组（8.8±2.5）%，空白对照组（5.7±1.8）%，siRNA组（56.3±4.9）%

P0.05］。结论转录辅助因子PC4可能参与了体外连续传代培养过程中MSCs增殖活性降低和衰老过程的调控。

Abstract

Objective To investigate the potential role of transcriptional positive coactivator 4 (PC4) in the senescence of rat bone marrow-derived mesenchymal stem cells (MSCs). Methods The expression of PC4 in MSCs was detected by RT-PCR and Western blotting. After the downregulation of PC4 expression by sequence specific siRNA

the proliferation of MSCs was assayed by CCK-8 and the senescence of MSCs was characterized by beta-galactosidase staining. Results After long-term in vitro culture

MSCs underwent replicative senescence and the expression of PC4 was significantly decreased. Downregulation of PC4 expression by RNA interference significantly inhibited the proliferation of MSCs and resulted in cellular senescence. Conclusion Transcriptional positive coactivator 4 may play a modulatory role in the proliferation and senescence of rat bone marrow-derived MSCs.

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