Mosapride promotes small intestine motility in acute high altitude-exposed rats

薛世祥; 彭贵勇; 杨红勤; 牟东; 代剑华; 袁月; 康秀峰

您当前的位置：

首页 >

文章列表页 >

Mosapride promotes small intestine motility in acute high altitude-exposed rats

更新时间：2026-01-09

- Mosapride promotes small intestine motility in acute high altitude-exposed rats
- Journal of Army Medical University Vol. 34, Issue 9, Pages: 801-804(2012)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R333.3;R364.4;R975
- Published：2012
- 稿件说明：
移动端阅览
薛世祥,彭贵勇,杨红勤,牟东,代剑华,袁月,康秀峰.莫沙必利对模拟急性高原暴露大鼠小肠动力的改善作用[J].陆军军医大学学报(原第三军医大学学报),2012,34(09):801-804.

　Xue Shixiang,Peng Guiyong,Yang Hongqin,Mou Dong,Dai Jianhua,Yuan Yue,Kang Xiufeng.Mosapride promotes small intestine motility in acute high altitude-exposed rats[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(09):801-804.
薛世祥,彭贵勇,杨红勤,牟东,代剑华,袁月,康秀峰.莫沙必利对模拟急性高原暴露大鼠小肠动力的改善作用[J].陆军军医大学学报(原第三军医大学学报),2012,34(09):801-804. DOI：

　Xue Shixiang,Peng Guiyong,Yang Hongqin,Mou Dong,Dai Jianhua,Yuan Yue,Kang Xiufeng.Mosapride promotes small intestine motility in acute high altitude-exposed rats[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(09):801-804. DOI：

摘要

目的观察莫沙必利对高原缺氧大鼠小肠动力及黏膜屏障的保护作用，初步探讨其对肠道急性缺氧损害的预防作用。方法将90只SD雄性大鼠按随机数字表法分为正常对照组、高原缺氧组、高原莫沙必利组；低压氧舱模拟海拔6 000 m，氧分压 (9.31±0.05) kPa，氧含量（19.6±0.2）%，建立大鼠急性缺氧模型

高原莫沙必利组采用莫沙必利灌胃，3次/d，每天剂量0.27 mg/kg。分别于10、24、48、72、168 h取小肠组织。检测急性高原缺氧应激后小肠组织一氧化氮（NO）、一氧化氮合酶（NOS）的含量变化；墨汁灌胃实验计算推进距离，检测小肠动力；RT-PCR检测小肠黏膜闭锁连接蛋白1（ZO-1） mRNA的表达情况。结果与同时间段正常对照组比，高原缺氧组NO、NOS含量增高，24、48、72、168 h NO含量差异明显（P0.05，P0.01），48、72、168 h NOS含量差异明显（P0.05，P0.01）；ZO-1 mRNA表达量降低，48、72、168 h差异明显（P0.05，P0.01）；高原莫沙必利组NO、NOS含量较正常对照组增高，仅48 h NO含量增高明显（P0.05）；高原缺氧组与高原莫沙必利组（168 h除外）各时间点小肠推进距离均较正常对照组明显降低（P0.05，P0.01）。各时间点高原莫沙必利组NO、NOS含量较高原缺氧组降低，72 h NO，72、168 h NOS含量差异明显（P0.05）；各时间点高原莫沙必利组小肠推进距离及ZO-1 mRNA的表达量较高原缺氧组增高，48、72、168 h的小肠推进距离及72、168 h ZO-1 mRNA的表达量差异明显（P0.05）。结论莫沙必利可有效促进小肠动力，减轻高原缺氧应激引起的小肠动力障碍及肠道黏膜屏障的损伤，可用于高原缺氧肠道损害的预防。

Abstract

Objective To determine the preventive effect of mosapride in the power of small intestine and mucosal barrier in rats in high altitude environment

and investigate its preventive role in intestinal injury induced by acute hypoxia. Methods A total of 90 SD male rats were randomly divided into 3 groups

that is

normal control group

hypoxia model group and hypoxia+mosapride treatment group (n=30). The rats from the 2 later groups were exposed to 6 000-meter high altitude in a hypobaric chamber under oxygen partial pressure (9.31±0.05) kPa and containing oxygen (19.6±0.2)% to establish rat model of acute hypoxia. At the same time

the animals of hypoxia+mosapride treatment group were gavaged with mosapride [0.27 mg/(kg·d)

3 times per day]. The rats were killed in 10

and 168 h respectively

and their small intestine tissues were taken for content of nitric oxide (NO) and activity of nitric oxide synthase (NOS). The motility of small intestine was evaluated by calculating the promotion distance of the ink which was fed in 30 min before sacrifice. RT-PCR was employed to detect the expression of ZO-1 in small intestine. Results Compared with the normal control group

NO content and NOS activity in the model group were significantly increased

and the expression of ZO-1 mRNA was significantly decreased

especially the expression in 48 h (P0.05). So did the promote distance of small intestine were significantly lower at all time points (P0.05). Though the hypoxia+mosapride group had above mentioned changes

mosapride obviously attenuated them when compared with model group. NO content at 72 h

NOS activity and ZO-1 mRNA expression at 72 and 168 h

and promotion distance of small intestine at 48

72 and 168 h in mosapride treatment group were significantly improved (P0.05). Conclusion Mosapride promotes the power of small intestine effectively

reduces the disorders of small intestine power and the damage of intestinal mucosal barrier induced by high altitude stress

and it can be used to prevent intestinal damage in altitude.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Hypoxia drives spermatogenic impairment by triggering lysosome-dependent death of Leydig cells via M1 macrophage polarization

Celecoxib improves right heart function in mice after acute high-altitude hypoxia exposure by increasing 12,13-diHOME level

Hypoxia characteristics and prognostic significance of glioblastoma MES-like subpopulation through multi-transcriptomics sequencing

Mutual regulation of HIF-1α and circ-UBE2G1 under hypoxic microenvironment promotes thyroid cancer metastasis

Hypoxic transcriptional phenotype and cellular ultrastructural changes of tumor-associated macrophages in gliomas

Related Author

MENG Dan

HE Wei

ZENG Jitao

ZHANG Wei

BAO Xinyu

LAI Xiaoyue

WAN Xiaoqin

TAN Yan

Related Institution

Institute of Immunology, College of Basic Medical Science，Army Medical University （Third Military Medical University）

Reproductive Medical Center， First Affiliated Hospital, College of Basic Medical Science，Army Medical University （Third Military Medical University）

Department of Ultrasonography, Second Affiliated Hospital， Army Medical University （Third Military Medical University）

Department of Pathophysiology， Faculty of High Altitude Military Medicine, Second Affiliated Hospital， Army Medical University （Third Military Medical University）

Department of Cardiovascular Medicine， First Affiliated Hospital, Second Affiliated Hospital， Army Medical University （Third Military Medical University）

⁰