Biological effect of ultra-microfractionated vs conventional radiotherapy on glioma cells

陈媛媛; 汤金梁; 龙海霞; 李光辉; 许建平

您当前的位置：

首页 >

文章列表页 >

Biological effect of ultra-microfractionated vs conventional radiotherapy on glioma cells

更新时间：2026-01-09

- Biological effect of ultra-microfractionated vs conventional radiotherapy on glioma cells
- Journal of Army Medical University Vol. 34, Issue 11, Pages: 1026-1030(2012)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R730.23;R730.55;R739.41
- Published：2012
- 稿件说明：
移动端阅览
陈媛媛,汤金梁,龙海霞,李光辉,许建平.低剂量超微分割与常规分割放疗对脑胶质瘤细胞的生物学效应比较[J].陆军军医大学学报(原第三军医大学学报),2012,34(11):1026-1030.

　Chen Yuanyuan,Tang Jinliang,Long Haixia,Li Guanghui,Xu Jianping.Biological effect of ultra-microfractionated vs conventional radiotherapy on glioma cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(11):1026-1030.
陈媛媛,汤金梁,龙海霞,李光辉,许建平.低剂量超微分割与常规分割放疗对脑胶质瘤细胞的生物学效应比较[J].陆军军医大学学报(原第三军医大学学报),2012,34(11):1026-1030. DOI：

　Chen Yuanyuan,Tang Jinliang,Long Haixia,Li Guanghui,Xu Jianping.Biological effect of ultra-microfractionated vs conventional radiotherapy on glioma cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(11):1026-1030. DOI：

摘要

目的比较低剂量超微分割放疗与常规分割放疗对脑胶质瘤细胞的作用效果。方法以低剂量率超微分割放疗（每0.2 Gy间隔3 min，剂量率为0.066 7 Gy/min）和常规分割（剂量率4～6 Gy/min）2种方法照射脑胶质瘤GL261细胞，分别在第1、2、3天采用彗星分析和流式细胞分析检测细胞DNA损伤、凋亡、细胞周期分布。结果彗星实验显示低剂量率超微分割放疗可产生与常规分割等效的细胞DNA损伤，2组间各时间点彗星荧光图像的尾部DNA百分含量(tail DNA％)、尾长(tail length)、尾距(tail moment)均数差异无显著性(P0.05)。细胞凋亡、存活情况显示超微分割组各时间点的细胞存活率较常规分割组低，差异有显著性 (P0.01)，2组细胞周期分布差异无显著性（P0.05）。 结论 低剂量率超微分割放疗对脑胶质瘤细胞GL261可产生与常规分割等效的DNA损伤，但对细胞的杀伤作用强于常规分割照射。该现象可能与低剂量率超微分割照射胶质瘤细胞后有不同的DNA损伤修复机制有关。

Abstract

Objective To compare the effect of ultra-microfractionated and conventional radiotherapy on glioma cells (GL261 cells). Methods GL261 cells underwent ultra-microfractionated radiotherapy at an interval of 3 min for each 0.2 Gy with a dose rate of 0.066 7 Gy/min and conventional radiotherapy at a dose rate of 4 to 6 Gy/min. Distribution of cell DNA damage

apoptosis and cell cycle was detected by comet assay and flow cytometry

respectively

on days 1

2 and 3 after treatment. Results Comet assay showed that ultra-microfractionated and conventional radiotherapy could cause similar DNA damages in GL261 cells. No significant difference was found in comet tail DNA %

tail length and tail moment at different time points between the two groups (P0.05). Flow cytometry showed that the cell survival rate was significantly lower at different time points after ultra-microfractionated radiotherapy than after conventional radiotherapy (P0.01). No significant difference was observed in distribution of cell cycle between the two groups (P0.05). Conclusion Ultra-microfractionated and conventional radiotherapy cause similar DNA damages in GL261 cells. However

the killing effect of ultra-microfractionated radiotherapy on GL261 cells is significantly stronger than that of conventional radiotherapy

which may be due to the difference in their DNA repairing mechanism.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of cinnamaldehyde on Bax/Bak and apoptosis of vascular endothelial cells in diabetic ulcers

LncRNA GAS5 inhibits glioma progression through miR-135b-5p/APC axis

Excessive mechanical stretch stress induces tendon cell apoptosis via mechanosensitive ion channel protein Piezo1

Defective hippocampal astrocyte and neuronal primary cilia function in offspring brain of pregnant rats with advanced age might be associated with promoting apoptosis through LKB1/AMPK pathway

Combined exposure to noise and CO induces adverse male reproductive effects in rats

Related Author

JIN Zheyu

XIE Chenlei

FAN Xinqi

YANG Shu

DONG Ruiyi

BAI Yanyu

DING Yarong

ZHOU Zhongzhi

Related Institution

Hunan Provincial Key Laboratory of Vascular Biology and Translational Medicine， Hunan University of Chinese Medicine

Department of Burn Wounds and Plastic Surgery， First Affiliated Hospital of Hunan University of Chinese Medicine

Neurological Diseases Center， the Third Affiliated Hospital of Chongqing Medical University

Department of Neurology Children’s Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics

⁰