D<sub>1</sub>-like dopamine receptor suppresses neuropeptide Y-induced proliferation in vascular smooth muscle cells

周永巧; 王震; 刘莉; 王微; 韩愈; 陈彩宇; 任红梅; 何多芬; 杨剑; 曾春雨

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D₁-like dopamine receptor suppresses neuropeptide Y-induced proliferation in vascular smooth muscle cells

更新时间：2026-01-09

- D₁-like dopamine receptor suppresses neuropeptide Y-induced proliferation in vascular smooth muscle cells
- Journal of Army Medical University Vol. 34, Issue 7, Pages: 581-584(2012)
- 作者机构：
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  CLC： R322.12;R329.28;R362
- Published：2012
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周永巧,王震,刘莉,王微,韩愈,陈彩宇,任红梅,何多芬,杨剑,曾春雨.多巴胺D1类受体对神经肽Y受体介导的促血管平滑肌细胞增殖的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(07):581-584.

　Zhou Yongqiao,Wang Zhen,Liu Li,Wang Wei,Han Yu,Chen Caiyu,Ren Hongmei,He Duofen,Yang Jian,Zeng Chunyu.D1-like dopamine receptor suppresses neuropeptide Y-induced proliferation in vascular smooth muscle cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(07):581-584.
周永巧,王震,刘莉,王微,韩愈,陈彩宇,任红梅,何多芬,杨剑,曾春雨.多巴胺D1类受体对神经肽Y受体介导的促血管平滑肌细胞增殖的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(07):581-584. DOI：

　Zhou Yongqiao,Wang Zhen,Liu Li,Wang Wei,Han Yu,Chen Caiyu,Ren Hongmei,He Duofen,Yang Jian,Zeng Chunyu.D1-like dopamine receptor suppresses neuropeptide Y-induced proliferation in vascular smooth muscle cells[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(07):581-584. DOI：

摘要

目的探讨多巴胺D

类受体对神经肽Y（neuropeptide Y，NPY）受体介导的Sprague-Dawley(SD)大鼠原代血管平滑肌细胞（vascular smooth muscle cells，VSMCs）增殖的影响。方法以NPY(10

-8

～10

-11

mol/L)刺激SD大鼠胸主动脉培养的VSMCs，观察在D

类多巴胺受体激动剂Fenoldopam(10

-8

mol/L)存在的情况下，神经肽Y促VSMCs增殖作用的变化。细胞增殖的检测采用[

]

胸腺嘧啶核苷（[

]

TdR）掺入率的变化表示及MTT方法。结果 NPY呈浓度依赖性促进SD大鼠VSMCs的异常增殖，最高增殖幅度达（77±9）%(P0.05)，该增殖作用由NPY Y

受体亚型介导。多巴胺D

类受体激动剂Fenoldopam对VSMCs无增殖影响，但Fenoldopam可抑制NPY Y

亚型受体介导VSMCs的增殖作用，该作用通过PKA途径发挥作用。结论多巴胺D

类受体激活抑制NPY 受体介导的促VSMCs增殖作用，可能参与心血管疾病的发生、发展过程。

Abstract

Objective To determine the effect of D

-like dopamine receptor on neuropeptide Y (NPY)-mediated proliferation in primary cultured vascular smooth muscle cells （VSMCs） derived from thoracic aorta of Sprague-Dawley(SD) rats. Methods After VSMCs were isolated from SD rat thoracic aorta and identified by morphology and immunocytocchemistry

the cells at passage 4 to 8 were treated by 10

-8

o 10

-11

mol/L NPY in presence or absence of D

-like receptor agonist fenoldopam (10

-8

mol/L)

blocker BIBP3226（10

-6

mol/L）

or antagonist SCH23390 （10

-8

mol/L）to observe NPY-mediated proliferation in VSMCs. The proliferation of VSMCs was investigated by [

]

-TdR incorporation and MTT assay. Results NPY resulted in an increased proliferation of VSMCs in a concentration-dependent manner

with a maximal proliferative amplitude of (77±9)% (P0.05). D

-like receptor agonist

fenoldopam

completely blocked the NPY Y

-mediated proliferation in VSMCs

although the agonist had no effect on the proliferation of VSMCs by itself

which might be through protein kinase A pathway. Conclusion Activation of D

-like receptor inhibits NPY Y

-mediated proliferation in VSMCs，which might be involved in the pathogenesis of cardiovascular diseases.

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D1-like dopamine receptor suppresses neuropeptide Y-induced proliferation in vascular smooth muscle cells

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摘要

Abstract

关键词

Keywords

references

D₁-like dopamine receptor suppresses neuropeptide Y-induced proliferation in vascular smooth muscle cells