Objective       To determine the inhibitory effects of resveratrol on TNF-α induced inflammatory damage in vascular endothelial cell line EA. hy926.       Methods       After the endothelial EA. hy926 cells were treated with TNF-α of different concentrations (0.01

0.1

1

2.5

5

10

20

50

100

200

and 400 ng/ml) for 24 h

cell viability and sICAM-1 release in the supernatant were measured by MTT and ELISA assay

respectively. Cells were pretreated with resveratrol of different concentrations (0.1

1

and 10 μmol/L) for 24 h

and then incubated with TNF-α (10 ng/ml) for another 24 h. sICAM-1 level in the the supernatant was determined by sICAM-1 ELISA kit. The mRNA expression of intercellular adhesion molecule-1 (ICAM-1) and tumor necrosis factor receptor-1 (TNFR1) were measured by qRT-PCR assay.       Results        After treated with TNF-α at high concentration (≥100 ng/ml)

cell viability was decreased significantly. Cell viability incubated with 200 ng/ml TNF-α was decreased by about 59.7% compared with control. When treated with a low concentration of TNF-α (10 ng/ml)

the mRNA levels of ICAM-1 and TNFR1 were increased markedly (P0.05). However

resveratrol pretreatment significantly (P0.01) inhibited the increase of ICAM-1 and TNFR1 mRNA expression induced by TNF-α in a dose-dependent manner.       Conclusion        Resveratrol might inhibit the TNF-α-induced inflammatory cytokine release and alleviate endothelial inflammation by inhibiting TNFR1-mediated signaling pathway.