(-)-epigallocatechin-3-gallate enhances low-dose cisplatin cytotoxicity to human small cell lung cancer cell line H446

赵汉玺; 胡旭东; 谢鹏; 孙新东; 于金明

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(-)-epigallocatechin-3-gallate enhances low-dose cisplatin cytotoxicity to human small cell lung cancer cell line H446

更新时间：2026-01-09

- (-)-epigallocatechin-3-gallate enhances low-dose cisplatin cytotoxicity to human small cell lung cancer cell line H446
- Journal of Army Medical University Vol. 34, Issue 2, Pages: 165-167(2012)
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  CLC： R73-36;R734.2;R979.1
- Published：2012
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赵汉玺,胡旭东,谢鹏,孙新东,于金明.表没食子儿茶素没食子酸酯增强低剂量顺铂对小细胞肺癌H446细胞株的细胞毒作用[J].陆军军医大学学报(原第三军医大学学报),2012,34(02):165-167.

　Zhao Hanxi,Hu Xudong,Xie Peng,Sun Xindong,Yu Jinming.(-)-epigallocatechin-3-gallate enhances low-dose cisplatin cytotoxicity to human small cell lung cancer cell line H446[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(02):165-167.
赵汉玺,胡旭东,谢鹏,孙新东,于金明.表没食子儿茶素没食子酸酯增强低剂量顺铂对小细胞肺癌H446细胞株的细胞毒作用[J].陆军军医大学学报(原第三军医大学学报),2012,34(02):165-167. DOI：

　Zhao Hanxi,Hu Xudong,Xie Peng,Sun Xindong,Yu Jinming.(-)-epigallocatechin-3-gallate enhances low-dose cisplatin cytotoxicity to human small cell lung cancer cell line H446[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(02):165-167. DOI：

摘要

目的探讨表没食子儿茶素没食子酸酯[(-)-epigallocatechin-3-gallate

EGCG

]

联合顺铂（Cisplatin，DDP）对小细胞肺癌细胞株H446的细胞毒作用及其机制。方法采用MTT法检测EGCG及DDP影响H446细胞增殖的量效和时效关系。采用流式细胞仪检测不同浓度EGCG、DDP及二者联合作用于H446细胞对细胞周期及凋亡的影响。结果 EGCG和DDP均可抑制H446细胞增殖，该增殖抑制作用呈浓度及时间依赖模式。不同浓度的EGCG（80、160 μmol/L）与DDP(1.0 μg/ml)联合应用时，可使H446细胞阻滞于G

/M期。EGCG可诱导H446细胞凋亡，并能增强DDP诱导H446细胞凋亡的作用。结论 EGCG能够抑制H446细胞增殖，诱导H446细胞凋亡，增强DDP诱导H446细胞凋亡的作用，其机制可能与G

/M期阻滞有关。

Abstract

Objective To determine the effect of (-)-epigallocatechin-3-gallate (EGCG) combined with cisplatin (DDP) on the cytotoxicity

apoptosis

and cell cycle in human small cell lung cancer cell line H446 cells. Methods Human small cell lung cancer H446 cells were treated with different concentrations of EGCG and DDP. The proliferation of cells was assayed by MTT assay. Flow cytometry (FCM) was used to detect cell cycle arrest. Cell apoptosis was analyzed by FCM with Annexin V/PI staining. Results Treatment of EGCG led to a significant inhibition in the proliferation of H446 cells in

a dose- and time-dependent manner. The percentage of cells at G

/M phase was increased markedly after treatment with EGCG and low-dose DDP. The apoptosis of H446 cells was induced by EGCG and low-dose DDP. The apoptotic rate was higher in the group of EGCG combined with DDP than in either of them alone. Conclusion EGCG can effectively inhibit the proliferation of H446 cells and induce cell apoptosis. Moreover

EGCG enhances H446 cells apoptosis induced by low-dose of DDP

which may be due to the cell cycle arrest at G

/M phase.

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