Effect of combined everolimus and cisplatin on cell cycle of gastric carcinoma cell line SGC7901

张瑞瑞; 刘磊; 刘莹; 孔庆兖

您当前的位置：

首页 >

文章列表页 >

Effect of combined everolimus and cisplatin on cell cycle of gastric carcinoma cell line SGC7901

更新时间：2026-01-09

- Effect of combined everolimus and cisplatin on cell cycle of gastric carcinoma cell line SGC7901
- Journal of Army Medical University Vol. 33, Issue 18, Pages: 1951-1955(2011)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R979.1;R735.2;R966
- Published：2011
- 稿件说明：
移动端阅览
张瑞瑞,刘磊,刘莹,孔庆兖.依维莫司联合顺铂对胃癌细胞株SGC7901细胞周期的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(18):1951-1955.

　Zhang Ruirui,Liu Lei,Liu Ying,Kong Qingyan.Effect of combined everolimus and cisplatin on cell cycle of gastric carcinoma cell line SGC7901[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(18):1951-1955.
张瑞瑞,刘磊,刘莹,孔庆兖.依维莫司联合顺铂对胃癌细胞株SGC7901细胞周期的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(18):1951-1955. DOI：

　Zhang Ruirui,Liu Lei,Liu Ying,Kong Qingyan.Effect of combined everolimus and cisplatin on cell cycle of gastric carcinoma cell line SGC7901[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(18):1951-1955. DOI：

摘要

目的探讨哺乳动物雷帕霉素靶蛋白特异性抑制剂依维莫司(everolimus，RAD001)对胃癌细胞株SGC7901细胞周期的影响、作用机制及其联合顺铂对SGC7901细胞抑制是否具有协同作用。方法体外培养SGC7901细胞，依维莫司单独及联合顺铂作用后，通过HE染色检测各组SGC7901细胞的形态学改变；流式细胞术检测细胞周期的改变；免疫细胞化学S-P法及Western blot检测细胞周期蛋白P21的表达。结果依维莫司能抑制SGC7901细胞增殖，且呈浓度剂量依赖性；联合用药对细胞生长抑制有协同效应；SGC7901细胞停滞在G

期的细胞比例增加

其中联合组G

期细胞比例最高（P0.05）。药物干预48 h后SGC7901细胞中的P21蛋白表达上调，联合组明显高于相应单独用药组

差异有统计学意义（P0.05）。结论依维莫司可以通过上调细胞周期蛋白P21的表达，阻滞胃癌SGC7901细胞周期进而抑制细胞增殖，联合用药对SGC7901细胞抑制具有协同效应。

Abstract

Objective To study the effect of a mTOR specific inhibitor

everolimus (RAD001)

on cell cycle of gastric carcinoma cell line SGC7901 and the synergic inhibitory effect of combined RAD001 and cisplatin on proliferation of SGC7901 cells. Methods After SGC7901 cells were cultured in vitro and stained with HE

their morphology was observed after treated with RAD001 alone or in combination with cisplatin

and their cell cycle was assayed by flow cytometry. Expression of P21 in SGC7901 cells was detected by S-P immunecellchemistry and Western blotting

respec

tively. Results RAD001 could inhibit the proliferation of SGC7901 cells in a concentration-dependent manner. Combined RAD001 and cisplatin showed a synergic inhibitory effect on the proliferation of SGC7901 cells. The proportion of SGC7901 cells at G

-G

was increased and significantly higher in combined RAD001 and cisplatin treatment group than in single RAD001 treatment group (P0.05). The expression level of P21 in SGC7901 cells was significantly higher 48 h after combined RAD001 and cisplatin treatment than after single RAD001 treatment (P0.05). Conclusion RAD001 can inhibit the proliferation of SGC7901 cells by up-regulating the expressions of P21 and blocking the cell cycle of SGC7901 cells. Combined RAD001 and cisplatin has a synergic inhibitory effect on the proliferation of SGC7901 cells.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Rosiglitazone induces cell cycle arrest in hepatocellular carcinoma cell line HepG2 via p38MAPK/p53/p21 pathway

Role of ARRDC3 in enterovirus D68-infected A549 cells

Effect of novel turtle shell decoction on cycle, apoptosis, CTGF/Itgα 5 pathway and expression of Col1a1 in rat hepatic stellate cells

Abnormal proliferation of Leydig cells in Sox30 knockout mice

Mechanism of hepatitis B virus X mediating SAMHD1 degradation to promote proliferation of hepatoma cells through AKT/p27 pathway

Related Author

张萌

彭利

乔治斌

何宏涛

周烨

李华怡

傅慧超

司俊卓

Related Institution

No data

⁰