Effect of specific integrin-linked kinase siRNA on growth of transplanted bladder cancer and expression of p-Akt and p-GSK3β

高娟; 朱军; 潘湘阳; 李红彦; 陈俊霞

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Effect of specific integrin-linked kinase siRNA on growth of transplanted bladder cancer and expression of p-Akt and p-GSK3β

更新时间：2026-01-09

- Effect of specific integrin-linked kinase siRNA on growth of transplanted bladder cancer and expression of p-Akt and p-GSK3β
- Journal of Army Medical University Vol. 33, Issue 18, Pages: 1947-1950(2011)
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  CLC： R73-362;R730.23;R737.14
- Published：2011
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高娟,朱军,潘湘阳,李红彦,陈俊霞.特异性ILK siRNA对膀胱癌移植瘤生长及p-Akt、p-GSK3β表达的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(18):1947-1950.

　Gao Juan,Zhu Jun,Pan Xiangyang,Li Hongyan,Chen Junxia.Effect of specific integrin-linked kinase siRNA on growth of transplanted bladder cancer and expression of p-Akt and p-GSK3β[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(18):1947-1950.
高娟,朱军,潘湘阳,李红彦,陈俊霞.特异性ILK siRNA对膀胱癌移植瘤生长及p-Akt、p-GSK3β表达的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(18):1947-1950. DOI：

　Gao Juan,Zhu Jun,Pan Xiangyang,Li Hongyan,Chen Junxia.Effect of specific integrin-linked kinase siRNA on growth of transplanted bladder cancer and expression of p-Akt and p-GSK3β[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(18):1947-1950. DOI：

摘要

目的探讨应用整合素连接激酶（integrin-linked kinase

ILK）特异siRNA沉默ILK基因的表达对膀胱癌裸鼠移植瘤生长及p-Akt、p-GSK3β表达的影响。方法实验分3组：BIU-87细胞组（正常BIU-87细胞）、BIU-87-NC组（转染阴性对照质粒的BIU-87细胞）、BIU-87 siRNA（转染干扰质粒的BIU-87细胞）。将ILK特异性siRNA转染人膀胱癌BIU-87细胞

筛选稳定表达ILK siRNA的BIU-87细胞，将其注入裸鼠皮下

检测裸鼠移植瘤的大小和质量，HE染色观察移植瘤组织的形态学变化，利用免疫组化技术检测移植瘤组织中微血管的变化及p-Akt和p-GSK3β的表达。结果 BIU-87 siRNA组较BIU-87与BIU-87-NC组，瘤体质量显著降低（P0.01），抑瘤率分别为64.73%和66.73%。BIU-87 siRNA组癌细胞较BIU-87与BIU-87-NC组体积减小，核浆比例明显缩小，细胞核染色较浅，核分裂相显著减少，BIU-87 siRNA组微血管较BIU-87与BIU-87-NC组明显减少甚至无血管出现，BIU-87 siRNA组p-Akt和p-GSK3β的表达呈弱阳性反应。结论特异性ILK siRNA能抑制膀胱癌裸鼠移植瘤的生长，并通过降低p-Akt和p-GSK3β的表达抑制膀胱癌裸鼠移植瘤的增殖。

Abstract

Objective To study the effect of specific integrin-linked kinase (ILK) siRNA on expression of p-Akt and p-GSK3β and growth of transplanted bladder cancer tumor xenografts. Methods Specific ILK siRNA was transfected into human bladder cancer BIU-87 cells. BIU-87 cells stably expressing ILK siRNA were selected and subcutaneously injected into nude mice. Size and weight of the transplanted bladder tumor in nude mice were measured. Morphology of tumor tissue was observed with HE staining. Change of micro-blood vessels in tumor tissue and expression of p-Akt and p-GSK3β were detected by immunohistochemistry. Results The weight of tumor was significantly lighter in mice with BIU-87 siRNA than in those with normal and negative control BIU-87 cells (P0.01) with a tumor inhibitory rate of 64.73% and 66.73%

respectively. The volume of cancer cells

the ratio of nuclear plasma

the staining of nuclei

and karyokinesis were significantly less in mice with BIU-87 siRNA than in those with normal and negative control BIU-87 cells. Less micro-blood vessels were observed in mice with BIU-87 siRNA than in those with normal and negative control BIU-87 cells or no blood vessels were found in mice with BIU-87 siRNA. The p-Akt and p-GSK3β were weakly expressed in mice with BIU-87 siRNA. Conclusion Specific ILK siRNA can inhibit the growth of transplanted bladder cancer in nude mice by decreasing the expression of p-Akt and p-GSK3β.

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