Objective     To study the correlation between polymorphism of leptin receptor (LEPR) gene Gln223Arg and type 2 diabetes mellitus (DM).      Methods     Three hundred and thirty-six patients with DM or other diseases

randomly sampled from 2 two hospitals in Chongqing

were divided into DM group (n=172) and control group (n=164). Risk factors for DM were investigated with case-control method.   Polymorphism of LEPR gene Gln223Arg was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)

and its relation with type 2 DM was analyzed.      Results     The genotype distribution frequency of AA

AG

and GG was 0.015

0307 and 0.678 respectively

while the allele distribution frequency of A and G was 0.202 and 0.798 respectively in DM group. The genotype distribution frequency of AA

AG

and GG accorded with the H-W balance (Chi square=3.081

P=0.079). The detection rate of genotype GG was higher while that of genotype AA and AG was lower in DM group than in control group (74.42% vs 60.98%

25.28% vs 39.02%

P0.05). Unconditional logistic regression analysis revealed that high total cholesterol (TC)

low high-density lipoprotein (HDL-C)

high waist-hip ratio (WHR)

family history of DM

and LEPR gene Gln223Arg mutation were the risk factors for type 2 DM.      Conclusion     Polymorphism of LEPR gene Gln223Arg may be correlated with type 2 DM.