Effect of medium molecular weight hydroxyethyl starch on neuropathic pain in rats after spinal cord injury

胡新宇; 陶军; 杨天德; 谢科宇

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Effect of medium molecular weight hydroxyethyl starch on neuropathic pain in rats after spinal cord injury

更新时间：2026-01-09

- Effect of medium molecular weight hydroxyethyl starch on neuropathic pain in rats after spinal cord injury
- Journal of Army Medical University Vol. 33, Issue 23, Pages: 2480-2484(2011)
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  CLC： R-332;R651.2;R977.7
- Published：2011
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胡新宇,陶军,杨天德,谢科宇.中分子量羟乙基淀粉对脊髓损伤后神经病理性疼痛的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(23):2480-2484.

　Hu Xinyu,Tao Jun,Yang Tiande,Xie Keyu.Effect of medium molecular weight hydroxyethyl starch on neuropathic pain in rats after spinal cord injury[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(23):2480-2484.
胡新宇,陶军,杨天德,谢科宇.中分子量羟乙基淀粉对脊髓损伤后神经病理性疼痛的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(23):2480-2484. DOI：

　Hu Xinyu,Tao Jun,Yang Tiande,Xie Keyu.Effect of medium molecular weight hydroxyethyl starch on neuropathic pain in rats after spinal cord injury[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(23):2480-2484. DOI：

摘要

目的研究中分子量羟乙基淀粉对脊髓损伤后损伤平面以下神经病理性疼痛的影响。方法 SD大鼠72只，采用随机数字法，分为4组，3组建大鼠脊髓（T10-11）中度撞击伤模型并经尾静脉分别给予生理盐水、低分子量羟乙基淀粉、中分子量羟乙基淀粉，余下1组仅去除相应椎板。行为学观察大鼠痛阈变化。术后测脊髓组织内伊文氏蓝含量，观察血脊髓屏障变化，Western blot观察胶质细胞活化情况，ELISA定量IL-1、IL-6、TNF-α表达水平。结果大鼠脊髓损伤后3 d脊髓组织伊文氏蓝含量测定示（生理盐水组：2.58±0.30；低分子量羟乙基淀粉组：2.38±0.48，中分子量羟乙基淀粉组：1.38±0.35）中分子量羟乙基淀粉组较生理盐水组和低分子量羟乙基淀粉组能有效缓解血脊髓屏障的异常开放（P0.05）；脊髓损伤后10 d神经胶质细胞形态学观察，中分子量羟乙基淀粉组较另2组可显著减轻损伤区巨噬细胞/小胶质细胞活化程度，并可降低损伤段脊髓组织IL-1、IL-6、TNF-α表达水平（P0.05）；术后10 d大鼠行为学观察，各手术组所测机械痛敏压力阈值较术前明显降低，中分子量羟乙基淀粉组较生理盐水组和低分子量羟乙基淀粉组治疗可使痛阈升高（P0.05）。结论中分子量羟乙基淀粉可能通过挽救通透性异常的血脊髓屏障，抑制损伤区域巨噬细胞/小胶质细胞的活化而缓解大鼠脊髓损伤后神经病理性疼痛。

Abstract

Objective To investigate the effect of medium molecular weight hydroxyethyl starch (HES 130/0.4

Voluven) on below-level neuropathic pain after spinal cord injury (SCI). Methods Using random number table

a total of 72 male adult SD rats were randomly and equally divided into four groups. Rats in three groups underwent moderate spinal cord contusion (T

10-11

) and were given normal saline

low molecular weight HES (HES 40) and me

dium molecular weight HES (HES 130/0.4

Voluven) through caudal vein

respectively; the other group of rats only underwent laminectomy and were set as control. In behavioral test

paw withdraw pressure threshold (PWPT) were measured to evaluate the central pain. Evans blue (EB) uptake in spinal cord tissues was measured to evaluate the disruption of blood spinal cord barrier. Levels of GFAP

Iba1 and ED1 were measured by Western blotting for the detection of astrocyte and macrophage/microglia activation. ELISA was applied to determine spinal expressions of IL-1

IL-6 and TNF-α. Results EB uptake in spinal cord tissues demonstrated that HES 130/0.4 effectively retrieved the compromised BSCB after 3 d of SCI as compared with normal saline and low molecular weight HES (Saline: 2.58±0.30; HES 40: 2.38±0.48; HES 130/0.4: 1.38±0.35; P0.05). Ten days after SCI

as compared with normal saline and low molecular weight HES

immunohistochemistry results showed that HES 130/0.4 significantly lessened macrophage/microglia activation，and ELISA showed HES 130/0.4 significantly reduced expressions of IL-1

IL-6 and TNF-α (P0.05). Behavioral test after ten-day SCI suggested that PWPT was dramatically reduced in all groups of rats

but HES 130/0.4 treatment could significantly raise the PWPT as compared with normal saline and low molecular weight HES (P0.05). Conclusion HES 130/0.4 could attenuated below-level neuropathic pain after spinal cord injury

and the mechanism maybe related to remedy of BSCB disruption and inhibition of macrophage/microglia activation.

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