Establishment and identificantion of a Tamoxifen-resistant cell line of MCF-7 human breast cancer

莫志强; 涂刚; 罗浩军; 李振华; 杨光伦

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Establishment and identificantion of a Tamoxifen-resistant cell line of MCF-7 human breast cancer

更新时间：2026-01-09

- Establishment and identificantion of a Tamoxifen-resistant cell line of MCF-7 human breast cancer
- Journal of Army Medical University Vol. 33, Issue 21, Pages: 2256-2259(2011)
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  CLC： R73-354;R734.2;R969.3
- Published：2011
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莫志强,涂刚,罗浩军,李振华,杨光伦.人乳腺癌MCF-7他莫昔芬耐药细胞株的建立及鉴定[J].陆军军医大学学报(原第三军医大学学报),2011,33(21):2256-2259.

　Mo Zhiqiang,Tu Gang,Luo Haojun,Li Zhenhua,Yang Guanglun.Establishment and identificantion of a Tamoxifen-resistant cell line of MCF-7 human breast cancer[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(21):2256-2259.
莫志强,涂刚,罗浩军,李振华,杨光伦.人乳腺癌MCF-7他莫昔芬耐药细胞株的建立及鉴定[J].陆军军医大学学报(原第三军医大学学报),2011,33(21):2256-2259. DOI：

　Mo Zhiqiang,Tu Gang,Luo Haojun,Li Zhenhua,Yang Guanglun.Establishment and identificantion of a Tamoxifen-resistant cell line of MCF-7 human breast cancer[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(21):2256-2259. DOI：

摘要

目的构建人乳腺癌细胞MCF-7他莫昔芬（Tamoxifen

TAM）耐药细胞模型，并鉴定其耐药性。方法采用高浓度短时间4-羟他莫昔芬(4-hydroxytamoxifen，OHT)冲击法，诱导人乳腺癌MCF-7/TAM耐药细胞株。通过细胞形态学观察、生长曲线、细胞倍增时间、药物毒性实验、细胞周期及凋亡相关蛋白BIK和Caspase-7的检测，评价MCF-7/TAM耐药细胞株的耐药性。结果成功构建MCF-7/TAM耐药细胞株，其耐药指数(resistance index，RI)为（13.88±2.93） (P0.05)。与其母细胞相比，MCF-7/TAM耐药细胞株不仅细胞形态发生了变化，生长增殖能力增强(P0.05)，细胞倍增时间缩短(P0.05)，而且对OHT介导的毒性效应有较强的抵抗能力(P0.05)，OHT不能促进MCF-7/TAM耐药细胞内BIK和Caspase-7蛋白的表达(P0.05)。结论通过高浓度短时间OHT冲击法诱导的MCF-7/TAM耐药细胞株对TAM产生一定的耐药性。

Abstract

Objective To establish a Tamoxifen (TAM)-resistant cell line of MCF-7 human breast cancer and to investigate the drug resistance of the cell line. Methods MCF-7 Tamoxifen-resistant (MCF-7/TAM-R) cell line was induced by a high concentration of 4-hydroxytamoxifen (OHT) treatment for a short period. Drug resistance of MCF-7/TAM-R cells were determined by cell morphology

cell growth curve

cell doubling time

drug toxicity tests

cell cycle and expression of apoptosis-related proteins BIK and Caspase-7. Results MCF-7/TAM-R cell line was successfully established

and its drug resistance index (RI) was 13.88±2.93 (P0.05). Compared with its parental cell line

MCF-7/TAM-R cell line exhibited a different cell morphology

a higher proliferation ability (P0.05)

a shorter cell doubling time (P0.05) and a stronger resistance to OHT-mediated cytotoxic effects (P0.05). OHT failed to induce the expression of BIK and Caspase-7 (P0.05) in MCF-7/TAM-R cell line. Conclusion MCF-7/TAM-R cell line induced by a high concentration of OHT for a short period has a significant resistance to TAM.

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李振华

涂刚

李维东

莫志强

杨光伦

罗浩军

柳满

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Related Institution

Department of Breast and Thyroid Surgery， First Affiliated Hospital， Army Medical University （Third Military Medical University）

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