Screening and evaluation of a newly designed siRNA targeting androgen receptor

张淼; 郭燕丽; 王洛夫; 李彦; 李锐

您当前的位置：

首页 >

文章列表页 >

Screening and evaluation of a newly designed siRNA targeting androgen receptor

更新时间：2026-01-09

- Screening and evaluation of a newly designed siRNA targeting androgen receptor
- Journal of Army Medical University Vol. 33, Issue 23, Pages: 2459-2462(2011)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R394-33;R394.3;R937.25
- Published：2011
- 稿件说明：
移动端阅览
张淼,郭燕丽,王洛夫,李彦,李锐.靶向于雄激素受体的siRNA筛选及效能评价[J].陆军军医大学学报(原第三军医大学学报),2011,33(23):2459-2462.

　Zhang Miao,Guo Yanli,Wang Luofu,Li Yan,Li Rui.Screening and evaluation of a newly designed siRNA targeting androgen receptor[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(23):2459-2462.
张淼,郭燕丽,王洛夫,李彦,李锐.靶向于雄激素受体的siRNA筛选及效能评价[J].陆军军医大学学报(原第三军医大学学报),2011,33(23):2459-2462. DOI：

　Zhang Miao,Guo Yanli,Wang Luofu,Li Yan,Li Rui.Screening and evaluation of a newly designed siRNA targeting androgen receptor[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(23):2459-2462. DOI：

摘要

目的设计并合成新的靶向于雄激素受体（AR）的siRNA序列，转染前列腺癌细胞筛选最佳干扰序列。 方法 设计并合成靶向于雄激素受体的siRNA，以不同浓度转染雄激素非依赖性前列腺癌细胞C4-2，确定最佳转染浓度。以最佳转染浓度转染细胞，分为siRNAⅠ组、siRNAⅡ组、siRNAⅢ组、阴性对照组、空白对照组和无关对照组。RT-PCR检测AR mRNA表达水平，Western blot检测AR蛋白表达水平。CCK-8分析绘制细胞生长曲线，观察C4-2细胞生长活性并筛选出抑制效果最佳的siRNA靶序列。结果以不同浓度的AR siRNA转染C4-2细胞后，细胞生长活性降低(P0.05)，并筛选出100 nmol/L的最佳转染浓度；RT-PCR和Western blot结果显示转染后AR的mRNA和蛋白表达水平显著下降(P0.05)；CCK-8法检测结果显示各组细胞增殖活性显著降低，其中siRNAⅠ的抑制效果最为明显(P0.05)。 结论 新设计合成的AR siRNA能通过沉默AR表达高效抑制C4-2细胞的增殖。

Abstract

Objective To design and synthesize siRNA sequences targeting androgen receptor (AR)

and to screen effective sequences by transfecting C4-2 cells. Methods Three pairs of siRNA sequences targeting AR were designed and synthesized according to the cDNA of AR

and were transfected into androgen-independent prostate carcinoma C4-2 cells to determine an optimal concentration. Experimental groups comprised of siRNAⅠ

siRNAⅡ

and siRNAⅢ

negative control

blank control and unrelated control. RT-PCR and Western blotting were applied to detect mRNA and protein expression of AR

respectively. Cell growth activity and the most effective siRNA sequence were evaluated based on cell growth curves by CCK-8 analysis. Results Cell growth activities of C4-2 cells transfected with different concentrations of AR siRNA (20 nmol/L

50 nmol/L

100 nmol/L and 200 nmol/L

respectively) were suppressed

and 100 nmol/L was the optimum concentration of AR siRNA (P0.05). Compared with those of the control groups

mRNA and protein levels of AR decreased significantly

and C4-2 cell growth activity was suppressed significantly in the groups of C4-2 cells transfected with AR siRNA (P0.05). The inhibitory effect of AR siRNAⅠ was the most significant than that of the others. Conclusion The newly designed AR siRNA can inhibit C4-2 cell proliferation by silencing AR expression.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Target prediction and preliminary validation of quercetin in treatment of endometriosis

Effect of LncRNA LINC01140 on proliferation and cell cycle in acute myeloid leukemia U937 cells

Sperm-associated antigen 6, independent on P53, regulates cell apoptosis of myelodysplastic syndromes induced by TRAIL

Vitamin E-coupled nanoparticles-delivered siRNA inhibits expression of hepatitis C virus core protein in vitro

Effect of cyclophilin A down-regulation on growth and metastasis of laryngeal squamous cell carcinoma in nude mice

Related Author

ZHANG Yi

WU Lulu

TANG Li

CUI Jiao

YUAN Wanjing

GONG Wenying

ZHU Jiao

LYU Xiuwei

Related Institution

the Second Affiliated Hospital of Hunan University of Chinese Medicine

the Central Hospital of Wuhan， Tongji Medical College， Huazhong University of Science and Technology

Rocket Force Medical Center of PLA

⁰