Effect of RNAi-mediated gene silencing of HER2/neu on proliferation and apoptosis in bladder cancer cell line BIU-87

李永生; 赵诚; 刘锦裕; 胡钊; 黄玉清

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Effect of RNAi-mediated gene silencing of HER2/neu on proliferation and apoptosis in bladder cancer cell line BIU-87

更新时间：2026-01-09

- Effect of RNAi-mediated gene silencing of HER2/neu on proliferation and apoptosis in bladder cancer cell line BIU-87
- Journal of Army Medical University Vol. 34, Issue 1, Pages: 20-23(2012)
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  CLC： R394-33;R730.23;R737.14
- Published：2012
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李永生,赵诚,刘锦裕,胡钊,黄玉清.RNA干扰HER2/neu对膀胱癌BIU-87细胞增殖和凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(01):20-23.

　Li Yongsheng,Zhao Cheng,Liu Jinyu,Hu Zhao,Huang Yuqing.Effect of RNAi-mediated gene silencing of HER2/neu on proliferation and apoptosis in bladder cancer cell line BIU-87[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(01):20-23.
李永生,赵诚,刘锦裕,胡钊,黄玉清.RNA干扰HER2/neu对膀胱癌BIU-87细胞增殖和凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(01):20-23. DOI：

　Li Yongsheng,Zhao Cheng,Liu Jinyu,Hu Zhao,Huang Yuqing.Effect of RNAi-mediated gene silencing of HER2/neu on proliferation and apoptosis in bladder cancer cell line BIU-87[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(01):20-23. DOI：

摘要

目的研究靶向HER2/neu基因的siRNA对膀胱癌BIU-87细胞增殖和凋亡的影响。方法化学合成的靶向HER2/neu基因siRNA在脂质体的介导下转染BIU-87细胞，实验分为HER2/neu siRNA组、空脂质体组、阴性siRNA序列组，以未转染BIU-87细胞为空白对照组。利用CCK-8法评价HER2/neu基因siRNA对BIU-87细胞体外生长的抑制作用

流式细胞术检测细胞凋亡情况，通过逆转录聚合酶链反应(RT-PCR)和Western blot法检测转染前后细胞中HER2/neu mRNA和蛋白表达水平的变化。结果转染HER2/neu siRNA后BIU-87细胞的存活率显著下降，从（82.37±0.90）%降低到（56.76±1.70）%，差异有统计学意义（P0.05）；同时能诱导细胞凋亡，HER2/neu siRNA组凋亡率达（45.60±0.70）%，与空白对照组、空脂质体组、阴性siRNA序列组比较差异有统计学意义（P0.05）；靶向HER2/neu基因siRNA能显著降低BIU-87细胞中HER2/neu mRNA和蛋白的表达（P0.05）。结论化学合成的靶向HER2/neu基因siRNA 能有效抑制HER2/neu基因在BIU-87细胞中的表达，进而能有效抑制BIU-87细胞的增殖和诱导凋亡的作用。

Abstract

Objective To investigate the effect of small interfering RNA (siRNA)-mediated gene silencing of HER2/neu on the proliferation and apoptosis in bladder cancer cell line BIU-87. Methods HER2/neu siRNA was chemically synthesized and transfected into BIU-87 cells mediated by liposome. BIU-87 cells with different treatments were divided into an HER2/neu siRNA group

an empty liposome group

a negative siRNA group and a non-transfection group was the control group. Inhibitory effect of HER2/neu siRNA on BIU-87 cell proliferation was assessed by CCK-8 assay. Cell apoptosis was examined by flow cytometry (FCM). mRNA and protein levels of HER2/neu in BIU-87 cells before and after transfection were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. Results BIU-87 cells transfected with HER2/neu siRNA showed a significant decrease of cell viability from (82.37±0.90)% to (56.76±1.70)% (P0.05) and cell apoptosis with a rate of (45.60±0.70)%

which was significantly different from that of the control group

empty liposome group and negative HER2/neu siRNA group (P0.05). The siRNA targeting HER2/neu gene significantly reduced mRNA and the protein expressions of HER2/neu in BIU-87 cells (P0.05). Conclusion Chemically synthesized siRNA targeting HER2/neu gene

which can effectively inhibit HER2/neu gene expression and subsequently inhibit cell proliferation and induce apoptosis in BIU-87 cells

is a potential target for gene therapy of bladder cancer.

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