Objective     To clone the gene of porcine apolipoprotein B mRNA editing enzyme

catalytic polypeptide-like 3F（APOBEC3F）and construct the eukaryotic expression vectors for its expression and identification in vitro.      Methods     Peripheral blood mononuclear cells (PBMC) were isolated from blood samples of 4 female healthy Wuzhishan porcine weighing 25 to 30 kg at the age of 21 weeks. RNA was extracted from porcine PBMC using the TRAZO regent. Coding region of the APOBEC3F gene was amplified by RT-PCR. Gene fragments were inserted into the recombinant eukaryotic expression vectors pDsRed1-N1 and modified pCDNA3-Flage-pA3F to construct the eukaryotic expression plasmids of pDsRed1-N1-pA3F and pCDNA3-Flage-pA3F expressed in PK-15 cells. The expression of target gene in PK-15 cells was detected by laser scanning confocal microscopy and Western blotting

respectively.      Results     The homology of target gene and published gene was 99%. Laser scanning confocal microscopy showed that the target gene was expressed in PK-15 cells and the expressed products were located in the cytoplasm. Western blot analysis revealed that the molecular weight of expressed target protein was consistent with the expected weight.      Conclusion     The eukaryotic expression vectors of porcine APOBEC3F has been constructed.