Biological characteristics of fibroblasts primarily cultured form breast precancerosis

您当前的位置：

首页 >

文章列表页 >

Biological characteristics of fibroblasts primarily cultured form breast precancerosis

更新时间：2026-01-09

- Biological characteristics of fibroblasts primarily cultured form breast precancerosis
- Journal of Army Medical University Vol. 34, Issue 1, Pages: 16-19(2012)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.9;R329-33;R329.28
- Published：2012
- 稿件说明：
移动端阅览
孙艳,彭琼乐,王黎阳,赵浏阳,袁泉,李俊,柳满然.乳腺癌前病变组织原代成纤维细胞生物学特征初步研究[J].陆军军医大学学报(原第三军医大学学报),2012,34(01):16-19.

　Sun Yan,Peng Qiongle,Wang Liyang,Zhao Liuyang,Yuan Quan,Li Jun,Liu Manran.Biological characteristics of fibroblasts primarily cultured form breast precancerosis[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(01):16-19.
孙艳,彭琼乐,王黎阳,赵浏阳,袁泉,李俊,柳满然.乳腺癌前病变组织原代成纤维细胞生物学特征初步研究[J].陆军军医大学学报(原第三军医大学学报),2012,34(01):16-19. DOI：

　Sun Yan,Peng Qiongle,Wang Liyang,Zhao Liuyang,Yuan Quan,Li Jun,Liu Manran.Biological characteristics of fibroblasts primarily cultured form breast precancerosis[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(01):16-19. DOI：

摘要

目的比较乳腺导管不典型增生成纤维细胞（atypical intraductal hyperplasia fibroblasts，AFs）与正常乳腺成纤维细胞（normal fibroblasts，NFs）、乳腺癌肿瘤相关成纤维细胞(cancer-associated fibroblasts，CAFs)在生物学特征上的差异。方法采用Ⅰ型胶原酶法分离并培养AFs、NFs和CAFs，免疫细胞荧光化学检测其粘连蛋白（fibronectin，FN）表达以鉴定细胞纯度。比较3种细胞的细胞生长曲线、细胞周期和周期蛋白cyclin D1、p21

waf1

及p53的变化。结果获得并鉴定了NFs、AFs、CAFs；其生长速度依次增加（P0.05），细胞周期S期百分比（8.24±2.47）、（16.94±4.77）、（33.31±7.90）依次增大（P0.05）；与NFs相比，AFs周期蛋白cyclin D1表达增加（P0.05），p21

waf1

和p53蛋白表达降低（P0.05）；与CAFs相比，AFs的周期蛋白cyclin D1没有升高（P0.05），p21

waf1

蛋白和p53蛋白表达更强（P0.05）。结论 AFs相对于NFs和CAFs，细胞生长增殖具有特异性，这种变化提示乳腺肿瘤的癌前病变阶段AFs可能是NFs逐步向CAFs转变的过渡阶段。

Abstract

Objective To investigate the biological characteristics of breast primary atypical intraductal hyperplasia fibroblasts (AFs) in tumorigenesis of breast cancer

normal mammary fibroblasts (NFs) and the breast primary cancer-associated fibroblasts (CAFs). Methods The primary human breast fibroblasts were isolated by collagenase Ⅰdigestion from normal mammary tissue

atypical intraductal hyperplasia and mammary tumor. The purity of these fibrob

lasts was identified by immunofluorescent staining using the specific first antibody to fibronectin

the fibroblast biomarker. The proliferation and cell cycle for each kind of fibroblasts was detected by MTT assay and flow cytometry. The expression of cyclin D1

p53 and p21

waf1

in the fibroblasts was detected by Western blotting. Results More than 95% of the isolated fibroblasts were positive to fibronectin. Comparing with NFs (8.24±2.47)

AFs (16.94±4.77) had higher growth rate and more cells at S-phase of cell cycle (P0.05). However

there were lower growth rate and lesser S-phase cells (P0.05) when compared these parameters in AFs with that in CAFs (33.31±7.90). Similar changes were found on the expression of cyclin D1

p21

waf1

and p53 among AFs

NFs and CAFs. Conclusion Different biological characteristics of AFs with NFs and CAFs indicate that AFs are on the way of transformation of NFs to CAFs in the precancerous lesion of breast cancer.

关键词

Keywords

references

0

Views

0

下载量

0

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Related Articles

Effect of siRNA mediated down-regulation of AnnexinⅡ on human breast cancer cell line MDA-MB-435S

1,25-dihydroxyvitamin D₃ induces cell cycle arrest of MDA-MB-231 cells transfected with exogenous ERα expression vector containing vitamin D response elements

A novel estrogen receptor expression stratification (low/moderate/high) based on Chinese population provides more accurate prognosis prediction for HR⁺/HER2^- early breast cancer patients

Downregulating RRM2B expression to increase radiation sensitivity of breast cancer cells

Characteristics of body image and their relationship with perceived stress, self-compassion and implicit attitudes in female breast cancer patients

Related Author

郭变琴

陈婷梅

文阳安

郎海滨

糜漫天

WANG Chengfang

ZHOU Yuqin

ZHANG Yanling

Related Institution

Department of Breast and Thyroid Surgery， First Affiliated Hospital， Army Medical University （Third Military Medical University）

⁰