Role of mitochondrial K<sub>ATP</sub> channel in pinacidil pretreatment-induced protective effect on blood flow and mitochondrial function of hemorrhagic shock rats

徐竞; 蓝丹; 李涛; 杨光明; 刘良明

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Role of mitochondrial K_ATP channel in pinacidil pretreatment-induced protective effect on blood flow and mitochondrial function of hemorrhagic shock rats

更新时间：2026-01-09

- Role of mitochondrial K_ATP channel in pinacidil pretreatment-induced protective effect on blood flow and mitochondrial function of hemorrhagic shock rats
- Journal of Army Medical University Vol. 33, Issue 23, Pages: 2441-2444(2011)
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  CLC： R361.3;R605.971;R966
- Published：2011
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徐竞,蓝丹,李涛,杨光明,刘良明.线粒体KATP在吡那地尔预处理后失血性休克大鼠组织血流灌注和线粒体功能保护效应中的作用[J].陆军军医大学学报(原第三军医大学学报),2011,33(23):2441-2444.

　Xu Jing,Lan Dan,Li Tao,Yang Guangming,Liu Liangming.Role of mitochondrial KATP channel in pinacidil pretreatment-induced protective effect on blood flow and mitochondrial function of hemorrhagic shock rats[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(23):2441-2444.
徐竞,蓝丹,李涛,杨光明,刘良明.线粒体KATP在吡那地尔预处理后失血性休克大鼠组织血流灌注和线粒体功能保护效应中的作用[J].陆军军医大学学报(原第三军医大学学报),2011,33(23):2441-2444. DOI：

　Xu Jing,Lan Dan,Li Tao,Yang Guangming,Liu Liangming.Role of mitochondrial KATP channel in pinacidil pretreatment-induced protective effect on blood flow and mitochondrial function of hemorrhagic shock rats[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(23):2441-2444. DOI：

摘要

目的观察吡那地尔（pinacidil）预处理对失血性休克大鼠血流灌注和线粒体功能的保护效应，及其与线粒体ATP激活钾通道[adenosine triphosphate（ATP）-activated potassium channels，K

ATP

]

的关系。方法 Wistar大鼠60只，随机数字表法分为：正常对照组、休克组、乳酸林格液（LR）复苏组、吡那地尔预处理组、5-HD组、格列本脲组，通过微血管张力测定技术、激光多普勒技术以及氧浓度测定技术，观察吡那地尔预处理（25 μg/kg，腹腔注射）对失血性休克和复苏过程中血管反应性和钙敏感性、脑、肝、肾重要器官的血流灌注和线粒体功能的保护作用，以及线粒体K

ATP

通道关闭剂5-HD 和格列本脲对其抑制作用。结果与LR复苏组相比，吡那地尔预处理可显著增高肠系膜上动脉（SMA）对去甲肾上腺素（NE）和Ca

的最大收缩力（E

max

）以及NE的升压效应，使其分别增高24.95%、15.48%和18.53%（P0.01），增加脑、肝、肾血流量，恢复肝、肾的线粒体呼吸控制率（respiration control ratio，RCR）和Na

-K

-ATP酶活性（P0.01）。5-HD和格列本脲均可显著抑制吡那地尔预处理的上述作用（P0.01）。结论吡那地尔预处理通过开放线粒体K

ATP

通道，改善失血性休克大鼠血管收缩反应，从而保护器官血流灌注和线粒体功能。

Abstract

Objective To investigate pinacidil pretreatment-induced protection on blood flow and mitochondrial function of hemorrhagic shock rats and role of mitochondrial K

ATP

channel in this process. Methods Sixty Wistar rats (200-230 g

male and female in half

) were randomly divided into six groups including sham operation group

shock group

lactated ringer’s solution (LR) resuscitation group

pinacidil pretreatment group (25 μg/kg

intraperitoneal injection)

5-hydroxydecanoate (5-HD) group and glibenclamide group. Microvascular tension measurement technique

laser-Doppler technique and oxygen concentration determination method were applied to observe the protective effect of pinacidil pretreatment on vascular reactivity

calcium sensitivity

blood flow and mitochondrial function in the brain

liver and kidney

and the inhibitory effects of mitochondrial K

ATP

channel blocker 5-HD and glibenclamide. Results As compared with LR resuscitation group

pinacidil pretreatment significantly increased the maximum contraction (E

max

) of superior mesenteric artery (SMA) for norepinephrine (NE) and Ca

and raised the presser effect of NE by 24.95%

15.48% and 18.53%

respectively (P0.01); Pinacidil pretreatment also improved blood flow of the brain

liver and kidney

mitochondrial respiration control ratio (RCR) and activity of Na

-K

-ATPase (P0.01). All these effects could be significantly blocked by 5-HD and glibenclamide (P0.01). Conclusion Pinacidil pretreatment-induced effect protects blood flow and mitochondrial function of hemorrhagic shock rats through mitochondrial K

ATP

channel.

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Related Institution

National Key Laboratory of Trauma and Chemical Poisoning， Department of War Trauma， Shock and Blood Transfusion， Daping Hospital， Army Medical University（Third Military Medical University）

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Role of mitochondrial KATP channel in pinacidil pretreatment-induced protective effect on blood flow and mitochondrial function of hemorrhagic shock rats

DOI：

摘要

Abstract

关键词

Keywords

references

Role of mitochondrial K_ATP channel in pinacidil pretreatment-induced protective effect on blood flow and mitochondrial function of hemorrhagic shock rats