Genotyping of 101 Mycobacterium tuberculosis isolates from pediatrics tuberculosis in Chongqing by multiple locus VNTR analysis

王均; 黄延风; 张爱华; 许红梅

您当前的位置：

首页 >

文章列表页 >

Genotyping of 101 Mycobacterium tuberculosis isolates from pediatrics tuberculosis in Chongqing by multiple locus VNTR analysis

更新时间：2026-01-09

- Genotyping of 101 Mycobacterium tuberculosis isolates from pediatrics tuberculosis in Chongqing by multiple locus VNTR analysis
- Journal of Army Medical University Vol. 33, Issue 13, Pages: 1408-1410(2011)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R372;R378.911;R394.3
- Published：2011
- 稿件说明：
移动端阅览
王均,黄延风,张爱华,许红梅.儿童结核病101例临床分离结核分枝杆菌多位点串联重复序列分型[J].陆军军医大学学报(原第三军医大学学报),2011,33(13):1408-1410.

　Wang Jun,Huang Yanfeng,Zhang Aihua,Xu Hongmei.Genotyping of 101 Mycobacterium tuberculosis isolates from pediatrics tuberculosis in Chongqing by multiple locus VNTR analysis[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(13):1408-1410.
王均,黄延风,张爱华,许红梅.儿童结核病101例临床分离结核分枝杆菌多位点串联重复序列分型[J].陆军军医大学学报(原第三军医大学学报),2011,33(13):1408-1410. DOI：

　Wang Jun,Huang Yanfeng,Zhang Aihua,Xu Hongmei.Genotyping of 101 Mycobacterium tuberculosis isolates from pediatrics tuberculosis in Chongqing by multiple locus VNTR analysis[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(13):1408-1410. DOI：

摘要

目的了解重庆地区儿童结核病中结核分枝杆菌临床分离株的数目可变串联重复序列（variable number tandem repeats

VNTR）的分布特征，寻找适合的VNTR位点组合。方法采用多位点串联重复序列（multiple locus VNTR analysis

MLVA）分型方法，选择标化的24个VNTR位点，对101例结核分枝杆菌临床分离株DNA进行检测，结果采用BioNumerics 6.1数据库软件进行聚类分析和单位点Hunter-Gaston分辨率指数（Hunter-Gaston index

HGI）分析，并比较分析国际推荐的分组（12、15、24位点）的基因分型鉴定能力。结果 MLVA分析结果显示24个VNTR位点在不同菌株中存在明显的多态性，101例结核分枝杆菌临床分离株可分为1个基因群83种基因型，其中69种基因型只有1个菌株，占68.32%（69/101），另有32株临床分离株表现出14种基因型，占31.68%（32/101），成簇率为17.82%； 24个VNTR位点的HGI具有较大差异（0.168~0.829），HGI能达到0.5以上的VNTR位点数有16个；24个VNTR位点进行不同的位点组合：12、15个和24个位点组合的HGI分别为0.995、0.996、0.996。结论重庆地区儿童结核病中结核分枝杆菌存在基因多态性，国际推荐的15个VNTR位点组合的MLVA分型方法适用于其流行病学的研究。

Abstract

Objective To study the distribution of the variable number tandem repeats (VNTR) in clinically isolated strains Mycobacterium tuberculosis (M. tuberculosis) from pediatrics tuberculosis in Chongqing and explore the effective loci combination of them by multiple locus VNTR analysis (MLVA). Methods Twenty-four standardized MLVA loci was used to type the 101 clinically isolated M. tuberculosis strains

and the results were analyzed by BioNumerics 6.1 software

which including dendrogram and Hunter-Gaston index (HGI) of 24 loci. The HGI of different loci set (12 loci

15 loci

and 24 loci) was evaluated. Results Twenty-four loci showed different polymorphism. All the 101 clinical isolates were typed into one group that displaying 83 genotypes

in which 69 strains (68.32%

69/101) were typed 69 genotypes respectively

and 32 strains (31.68%) were typed 14 genotypes which showed cluster rate of 17.82%. The HGI of 24 loci was from 0.168 to 0.829

the number of VNTR loci with HGI higher than 0.5 was 16. Three loci set displayed different polymorphism. HGI of 12 loci

15 loci

and 24 loci set were 0.995

0.996

and 0.996 respectively. Conclusion The clinical strains of M. tuberculosis isolated from pediatrics tuberculosis in Chongqing show definite polymorphism and the standardization 15 loci VNTR set of MLVA can be used in the molecular epidemiological study of these strains in Chongqing.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Advances in deep learning and MRI co-assisted genotyping of brain gliomas

Polymorphisms of pepA and PPE18 genes in clinical isolates of Mycobacterium tuberculosis in children

Establishment of a risk prediction for tuberculosis infection model in HIV-infected/AIDS patients

Characteristics of HPV infection and value of its combination with TCT in screening cervical lesions: report based on 14 962 women taking physical examination

Genotyping of extended spectrum beta lactamases of 33 Escherichia coli strains from several hospitals of Guiyang City

Related Author

CHEN Junshan

CUI Shaoguo

朱朝敏

郑改焕

杨震华

黄延风

苏薇

蒋秋

Related Institution

College of Computer and Information Science， Chongqing Normal University

⁰