Construction of scaffold for treatment of articular cartilage defect using high-concentration collagen Ⅱ

崔运利; 王富友; 谭洪波; 张颖; 熊娟; 杨柳

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Construction of scaffold for treatment of articular cartilage defect using high-concentration collagen Ⅱ

更新时间：2026-01-09

- Construction of scaffold for treatment of articular cartilage defect using high-concentration collagen Ⅱ
- Journal of Army Medical University Vol. 33, Issue 14, Pages: 1511-1513(2011)
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  CLC： R318.08;R322.71;R341
- Published：2011
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崔运利,王富友,谭洪波,张颖,熊娟,杨柳.应用高浓度Ⅱ型胶原蛋白构建组织工程软骨支架[J].陆军军医大学学报(原第三军医大学学报),2011,33(14):1511-1513.

　Cui Yunli,Wang Fuyou,Tan Hongbo,Zhang Ying,Xiong Juan,Yang Liu.Construction of scaffold for treatment of articular cartilage defect using high-concentration collagen Ⅱ[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(14):1511-1513.
崔运利,王富友,谭洪波,张颖,熊娟,杨柳.应用高浓度Ⅱ型胶原蛋白构建组织工程软骨支架[J].陆军军医大学学报(原第三军医大学学报),2011,33(14):1511-1513. DOI：

　Cui Yunli,Wang Fuyou,Tan Hongbo,Zhang Ying,Xiong Juan,Yang Liu.Construction of scaffold for treatment of articular cartilage defect using high-concentration collagen Ⅱ[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(14):1511-1513. DOI：

摘要

目的构建有利于种子细胞黏附、增殖和分化的软骨组织工程海绵支架。方法依据天然软骨主要成分，提取透明软骨Ⅱ型胶原蛋白，将提取的胶原蛋白通过高速离心进行浓缩，真空冷冻干燥制得海绵支架材料，应用化学交联剂EDC/NHS进行化学交联，对支架采用组织化学染色、体外降解实验、扫描电镜和接种细胞静态培养的方法进行相关检测。结果支架具有良好的孔隙结构，孔径40～130 μm，孔隙率在90%以上，细胞在支架上增殖、分化良好。结论构建的高浓度Ⅱ型胶原蛋白海绵支架具有良好的生物相容性，有较好的应用前景。

Abstract

Objective To construct a sponge scaffold for cell adhesion

proliferation and differentiation in cartilage tissue engineering. Methods Collagen Ⅱ was extracted from hyaline cartilage

and was then subjected to high-speed centrifugation and vacuum freeze-drying to obtain a sponge scaffold. The sponge scaffold was soaked in 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) for cross-linking. The sponge scaffold was evaluated by scanning electron microscopy

immunohistochemistry

degradation test and cell adhesion and culture. Results The sponge scaffold had a good pore structure

with pore sizes of 40-130 μm and porosity above 90%. Good cell proliferation and differentiation were found on the scaffold. Conclusion The sponge scaffold constructed from high-concentration collagenⅡ has excellent biocompatibility

and shows great promise in the treatment of articular cartilage defect.

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