Silencing nucleostemin in EC9706 cells inhibits tumor growth and expression of EGF and EGFR in xenograft tumor in nude mice

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Silencing nucleostemin in EC9706 cells inhibits tumor growth and expression of EGF and EGFR in xenograft tumor in nude mice

更新时间：2026-01-09

- Silencing nucleostemin in EC9706 cells inhibits tumor growth and expression of EGF and EGFR in xenograft tumor in nude mice
- Journal of Army Medical University Vol. 33, Issue 13, Pages: 1370-1373(2011)
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  CLC： R394.3;R730.23;R735.1
- Published：2011
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张功员,关文池,李晟磊,张巧,吴卫东.沉默EC9706细胞NS基因对裸鼠移植瘤组织中EGF、EGFR基因表达的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(13):1370-1373.

　Zhang Gongyuan,Guan Wenchi,Li Shenglei,Zhang Qiao,Wu Weidong.Silencing nucleostemin in EC9706 cells inhibits tumor growth and expression of EGF and EGFR in xenograft tumor in nude mice[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(13):1370-1373.
张功员,关文池,李晟磊,张巧,吴卫东.沉默EC9706细胞NS基因对裸鼠移植瘤组织中EGF、EGFR基因表达的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(13):1370-1373. DOI：

　Zhang Gongyuan,Guan Wenchi,Li Shenglei,Zhang Qiao,Wu Weidong.Silencing nucleostemin in EC9706 cells inhibits tumor growth and expression of EGF and EGFR in xenograft tumor in nude mice[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(13):1370-1373. DOI：

摘要

目的建立裸鼠食管癌移植瘤模型，探讨由RNAi诱导的EC9706细胞核干细胞因子（nucleostemin

NS）基因沉默对裸鼠移植瘤组织中EGF、EGFR基因表达的影响。方法裸鼠15只，采用STATA 8.0编制随机化分组程序，均分为3组，分别皮下注入pRNAT-U6.1-siNS2转染的EC9706 细胞（siRNA 干预组），pRNAT-U6.1-siC 转染的EC9706细胞（无关siRNA对照组）以及正常EC9706细胞（空白对照组）

接种5周，观察移植瘤生长情况，应用免疫组织化学方法和原位杂交技术检测裸鼠移植瘤组织中NS、EGF、EGFR基因蛋白和mRNA的表达。结果无关siRNA对照组及空白对照组第4天于接种部位出现肉眼可见的肿块，siRNA 干预组于第6天在相应部位发现肉眼可见肿块，第5周各组裸鼠成瘤率均为100%。siRNA干预组、无关siRNA对照组及空白对照组裸鼠移植瘤组织中NS、EGF、EGFR蛋白和mRNA的阳性表达率比较差异显著(P0.05)。相对于无关siRNA对照组和空白对照组，siRNA干预组裸鼠肿瘤组织中NS蛋白及mRNA的阳性表达率均降低。结论 EC9706细胞沉默NS基因可降低裸鼠体内EGF、EGFR基因的表达。

Abstract

Objective To investigate the effect of silencing nucleostemin (NS) in EC9706 cells induced by RNAi on the expression of epidermal growth factor (EGF) and its receptor (EGFR). Methods Fifteen nude mice were divided into three groups

each group contains five mice. Three different kinds of cells

EC9706 cells transfected by pRNAT-U6-1-siNS2

or by pRNAT-U6.1-siC and normal EC9706 cells were injected into the hypodermis tissues of corresponding mice respectively. Five weeks later after the injection

the sizes of xenograft tumors in these mice were measured

and the protein and mRNA expression of NS

EGF and EGFR in the xenograft tumor were detected by immunohistochemistry and RT-PCR. Results Visible tumors were observed in the injected position after 4 d in transfected pRNAT-U6.1-siC and normal EC9706 cells groups

and after 6 d in transfected pRNAT-U6-1-siNS2 EC9706 cells group. In 5th week

the tumorigenesis were 100%. The protein and mRNA levels of NS

EGF and EGFR were lower in transfected pRNAT-U6-1-siNS2 EC9706 cells group than in the other two groups (P0.05). Conclusion Silencing NS of EC9706 cells inhibits the growth of exnograft tumor in nude mice and decreases the expression of EGF gene and EGFR gene. Silencing NS gene may be a new strategy for the treatment of esophagus caicinoma.

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