Quercetin combined with cisplatin on proliferation and apoptosis in human ovarian cancer SKOV-3 cells

彭慧霞; 潘艳艳; 邢兰瑛; 王艳华; 蔡东阁; 杨雪梅; 李虹

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Quercetin combined with cisplatin on proliferation and apoptosis in human ovarian cancer SKOV-3 cells

更新时间：2026-01-09

- Quercetin combined with cisplatin on proliferation and apoptosis in human ovarian cancer SKOV-3 cells
- Journal of Army Medical University Vol. 33, Issue 15, Pages: 1606-1609(2011)
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  CLC： R737-31;R73-361;R979.11
- Published：2011
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彭慧霞,潘艳艳,邢兰瑛,王艳华,蔡东阁,杨雪梅,李虹.槲皮素联合顺铂对人卵巢癌SKOV-3细胞增殖及凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(15):1606-1609.

　Peng Huixia,Pan Yanyan,Xing Lanying,Wang Yanhua,Cai Dongge,Yang Xuemei,Li Hong.Quercetin combined with cisplatin on proliferation and apoptosis in human ovarian cancer SKOV-3 cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(15):1606-1609.
彭慧霞,潘艳艳,邢兰瑛,王艳华,蔡东阁,杨雪梅,李虹.槲皮素联合顺铂对人卵巢癌SKOV-3细胞增殖及凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(15):1606-1609. DOI：

　Peng Huixia,Pan Yanyan,Xing Lanying,Wang Yanhua,Cai Dongge,Yang Xuemei,Li Hong.Quercetin combined with cisplatin on proliferation and apoptosis in human ovarian cancer SKOV-3 cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(15):1606-1609. DOI：

摘要

目的探讨槲皮素单独及联合顺铂对人卵巢癌SKOV-3细胞增殖与凋亡的影响，并初步探讨其作用机制。方法选取对数生长期的卵巢癌SKOV-3细胞作为研究对象，实验分6组：阴性对照组（生理盐水），槲皮素低（25 μmol/L）、中（50 μmol/L）、高剂量组（100 μmol/L），顺铂组（10 μmol/L），联合组（槲皮素50 μmol/L+顺铂10 μmol/L）。采用MTT法检测不同浓度槲皮素单独及联合顺铂对SKOV-3细胞的增殖抑制作用；流式细胞仪检测细胞周期分布及细胞凋亡率；免疫细胞化学法检测槲皮素作用于SKOV-3细胞Bcl-2及MtP53蛋白表达水平的变化。结果与阴性对照组相比，槲皮素可明显抑制SKOV-3细胞的增殖，并具有显著的时间和剂量依赖关系，槲皮素高剂量组在72 h的抑制率可达63.49%（P0.05）；流式细胞仪分析发现槲皮素可使G

期细胞明显增多，S期细胞明显减少（P0.05），提示其将细胞周期阻滞于G

期；槲皮素还可明显诱导SKOV-3细胞凋亡，高剂量组的凋亡率可达29.51%；槲皮素作用后，Bcl-2及MtP53表达则明显降低（P0.05）。结论槲皮素在体外可抑制SKOV-3细胞增殖，阻滞细胞周期，诱导细胞凋亡，其机制可能与降低凋亡相关蛋白MtP53和Bcl-2的蛋白表达水平有关；槲皮素与顺铂联用有协同作用。

Abstract

Objective To determine the effect of quercetin combined with cisplatin on the proliferation and apoptosis in SKOV-3 ovarian cancer cells. Methods Ovarian cancer SKOV-3 cells at the logarithmic growth phase were treated with quercetin at 25

or 100 μmol/L respectively

with 10 μmol/L DDP

or with 50 μmol/L quercetin and 10 μmol/L DDP together

respectively for 24

48 and 72 h. The cell proliferation was evaluate

d by MTT assay

the rate of cell apoptosis and the cell-cycle distribution was detected by flow cytometry

and the expression of P53 and Bcl-2 were analyzed by immunocytochemical assay. Results Quercetin resulted in an decreased of the proliferation in SKOV-3 cells in a dose- and time-dependent manner

and the inhibitory rate in the 100 μmol/L quercetin for 72 h was 63.49% （P0.05）. Flow cytometry showed that the proportions of G

phase and of S phase were reduced after quercetin exposure（P0.05）. Quercetin also increased the apoptotic rate of SKOV-3 cells significantly and the highest apoptotic rate was 29.51%（P0.05）. Immunocytochemical assay showed that the expression of MtP53 and Bcl-2 proteins in the treated cells was significantly decreased compared with the control（P0.05）. Conclusion Quercetin

especially combined with cisplatin

significantly inhibits the proliferation and induces apoptosis of cervical cancer cells

which might be due to the down-regulation of Bcl-2 and MtP53.

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