Expression correlation between spermidine/spermine-N1-acetyltransferase 2 and hypoxia inducible factor-1α in cerebral cortex of rat after cardiopulmonary resuscitation

曾维威; 张献全

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Expression correlation between spermidine/spermine-N1-acetyltransferase 2 and hypoxia inducible factor-1α in cerebral cortex of rat after cardiopulmonary resuscitation

更新时间：2026-01-09

- Expression correlation between spermidine/spermine-N1-acetyltransferase 2 and hypoxia inducible factor-1α in cerebral cortex of rat after cardiopulmonary resuscitation
- Journal of Army Medical University Vol. 33, Issue 10, Pages: 1040-1043(2011)
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  CLC： R654.1;R322.11;R329.26
- Published：2011
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曾维威,张献全.心肺复苏后大鼠脑组织精脒/精胺-N1-乙酰基转移酶2与HIF-1α表达的动态变化规律[J].陆军军医大学学报(原第三军医大学学报),2011,33(10):1040-1043.

　Zeng Weiwei,Zhang Xianquan.Expression correlation between spermidine/spermine-N1-acetyltransferase 2 and hypoxia inducible factor-1α in cerebral cortex of rat after cardiopulmonary resuscitation[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(10):1040-1043.
曾维威,张献全.心肺复苏后大鼠脑组织精脒/精胺-N1-乙酰基转移酶2与HIF-1α表达的动态变化规律[J].陆军军医大学学报(原第三军医大学学报),2011,33(10):1040-1043. DOI：

　Zeng Weiwei,Zhang Xianquan.Expression correlation between spermidine/spermine-N1-acetyltransferase 2 and hypoxia inducible factor-1α in cerebral cortex of rat after cardiopulmonary resuscitation[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(10):1040-1043. DOI：

摘要

目的研究心肺复苏后大鼠脑组织中精脒/精胺-N1-乙酰基转移酶2（spermidine/spermine-N1-acetyltransferase2

SSAT2）与缺氧诱导因子-1α（hypoxia inducible factor 1 alpha

HIF-1α）表达的动态变化规律，分析二者相关性。方法 88只成年SD大鼠随机数字表法分为正常对照组（n=8）、假手术组（n=40）和心肺复苏组（n=40）。其中假手术组和心肺复苏组再分为1、8、24、48、72 h 5个亚组，每亚组8只。采用RT-PCR、Western blot法分别检测脑组织中HIF-1α mRNA和HIF-1α、SSAT2蛋白的表达。结果 SSAT2的表达在复苏后明显降低，1 h[(0.314±0.027)

P0.01]表达最低，随后逐渐升高(P0.01)，至72 h[(0.731±0.024)

P0.05]时回升到正常对照组水平（0.736±0.02），而假手术组SSAT2的表达与正常对照组相比无显著变化(P0.05)。HIF-1α mRNA的表达在复苏后1 h[(0.245±0.021)

P0.05]时降低，但8 h[(0.430±0.019)

P0.05]时明显增强，后24 h[(0.387±0.018)

P0.05]、48 h[(0.385±0.019)

P0.05]下降，至72 h时回落到正常对照组水平(0.343±0.022)；但HIF-1α蛋白在复苏后1 h[(0.755±0.012)

P0.01]显著增高，随后逐渐下降(P0.01)，至72 h[(0.033±0.002)

P0.01]时仅轻度增高，而在正常对照组和假手术组未能检测出HIF-1α表达。SSAT2表达与HIF-1α表达在复苏后72 h内呈负性相关(r=-0.945，P0.01)，SSAT2表达与HIF-1α mRNA表达在复苏后8~48 h呈负相关(r=-0.614，P=0.01)。结论在心肺复苏后一定时间内，大鼠脑组织SSAT2 表达减弱，HIF-1α mRNA及蛋白表达增加，两者呈负相关表达，提示SSAT2对HIF-1α蛋白、HIF-1α mRNA可能起负性调节作用。

Abstract

Objective To study the expression correlation between spermidine/spermine-N1-acetyltransferase 2 (SSAT2) and hypoxia inducible factor-1α (HIF-1α) in the cerebral cortex of rat after cardiopulmonary resuscitation (CPR). Methods Eighty-eight adult SD rats were randomized into a control group (n=8)

a sham operation group (n=40)

and a CPR group (n=40). The sham operation group and CPR group each were further divided into 1

and 72 h subgroups (n=8). In the CPR group

cardiac arrest (CA) was induced by bolus injection of 4℃ 10% potassium chloride solution through the external jugular vein

and cardiopulmonary resuscitation began 5 min after CA. The mRNA expression of HIF-1α and the protein expression of SSAT2 and HIF-1α in cerebral cortex were measured by RT-PCR and western blotting in 1

and 72 h after return of spontaneous circulation (ROSC). Results The protein expression of SSAT2 in the CPR group decreased markedly in 1 h after ROSC (0.314±0.027

P0.01)

then increased gradually

and returned to the normal level in 72 h after ROSC (0.731±0.024

P0.05)

and that in the sham operation group showed no significant difference from that in the control group (0.736±0.02) (P0.05). The mRNA expression of HIF-1α in the CPR group decreased in 1 h after ROSC (0.245±0.021

P0.05)

reached the peak level in 8 h after ROSC (0.430±0.019

P0.05)

decreased in 24 and 48 h after ROSC (0.387±0.018

P0.05; 0.385±0.019

P0.05)

and returned to the normal level in 72 h after ROSC (0.343±0.022

P0.05). The protein expression of HIF-1α in the CPR group increased significantly in 1 h after ROSC (0.755±0.012

P0.01)

then decreased gradually

and was weakly positive in 72 h after ROSC (0.033±0.002

P0.01). The protein expression of HIF-1α was not detected in the control group and sham operation group. The protein expression of SSAT2 was negatively correlated with the protein expression of HIF-1α within 72 h after ROSC (r=-0.945

P0.01) and the mRNA expression of HIF-1α between in 8 h and 48 h after ROSC (r=-0.614

P=0.01). Conclusion The protein expression of SSAT2 decreases after ROSC

while the mRNA and protein expression of HIF-1α increases

indicating that SSAT2 may regulate the mRNA and protein expression of HIF-1α negatively.

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