Effect of exogenous CD147 protein on synthesis and secretion of matrix metalloproteinases-2 and 9 during differentiation and maturation of osteoclasts in vitro

余世明; 初同伟; 廖通权; 胡旭; 刘玉刚; 周跃

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Effect of exogenous CD147 protein on synthesis and secretion of matrix metalloproteinases-2 and 9 during differentiation and maturation of osteoclasts in vitro

更新时间：2026-01-09

- Effect of exogenous CD147 protein on synthesis and secretion of matrix metalloproteinases-2 and 9 during differentiation and maturation of osteoclasts in vitro
- Journal of Army Medical University Vol. 33, Issue 14, Pages: 1492-1496(2011)
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  CLC： R336;R329.28;R392.3
- Published：2011
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余世明,初同伟,廖通权,胡旭,刘玉刚,周跃.CD147对体外破骨细胞分化过程中基质金属蛋白酶-2、基质金属蛋白酶-9分泌的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(14):1492-1496.

　Yu Shiming,Chu Tongwei,Liao Tongquan,Hu Xu,Liu Yugang,Zhou Yue.Effect of exogenous CD147 protein on synthesis and secretion of matrix metalloproteinases-2 and 9 during differentiation and maturation of osteoclasts in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(14):1492-1496.
余世明,初同伟,廖通权,胡旭,刘玉刚,周跃.CD147对体外破骨细胞分化过程中基质金属蛋白酶-2、基质金属蛋白酶-9分泌的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(14):1492-1496. DOI：

　Yu Shiming,Chu Tongwei,Liao Tongquan,Hu Xu,Liu Yugang,Zhou Yue.Effect of exogenous CD147 protein on synthesis and secretion of matrix metalloproteinases-2 and 9 during differentiation and maturation of osteoclasts in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(14):1492-1496. DOI：

摘要

目的建立体外破骨细胞（osteoclasts，OCs）诱导分化模型，研究CD147对体外破骨细胞分化过程中基质金属蛋白酶-2（matrix metalloproteinases 2，MMP-2）和基质金属蛋白酶-9（matrix metalloproteinases 9， MMP-9）合成与分泌的影响。方法采用健康成人外周血分离单个核细胞贴壁培养，应用巨噬细胞集落刺激因子（macrophage colony stimulating factor，M-CSF）与NF-κB受体活化因子配体(receptor or activator of NF-κB ligand，RANKL）诱导单个核细胞向Ocs分化。实验分为对照组（RANKL+M-CSF）与蛋白诱导组（RANKL+M-CSF+CD147蛋白）。应用抗酒石酸酸性磷酸酶染色（tartrate-resistant acid phosphatase，TRAP）及骨吸收实验检测鉴定OCs的分化成熟，Real-time PCR技术检测CD147、MMP-2和MMP-9 mRNA在破骨前体细胞（osteoclast precursor cells，OPCs）中表达变化，酶联免疫吸附实验（ELISA）检测细胞上清液中MMP-2、MMP-9的表达。结果 ①TRAP染色和骨吸收实验检测到破骨样细胞形成并具有骨吸收功能

建立体外Ocs诱导分化模型成功。②在24、48 h蛋白诱导组CD147 mRNA [(1.344±0.207)、(1.816±0.222)]、MMP-2 mRNA[(1.187±0.077)、(1.437±0.231)]及MMP-9 mRNA [(1.128±0.107)、( 2.353±0.679)]的相对表达量均高于相应的对照组（P0.05），且MMP-2、MMP-9 mRNA的相对表达量与CD147 mRNA的表达量呈正相关（r分别为0.818、0.758，P0.01）。③24、48 h蛋白诱导组MMP-2蛋白[（386.71±40.66）、（367.35±20.72）μg/L]、MMP-9蛋白[（1 177.78±66.42）、（1 514.37±68.56）μg/L]表达均高于相应对照组（P0.05），蛋白诱导组MMP-2蛋白的表达在24 h与48 h间无统计学差异（t=1.190，P0.05），而MMP-9蛋白的表达在48 h显著高于24 h（t=7.589，P0.05）。结论外源性CD147蛋白可以促进OCs分化成熟过程中MMP-2 及MMP-9的合成与分泌，但MMP-2分泌的增加不呈时间依赖性。

Abstract

Objective To establish a model of differentiation and maturation of osteoclasts (OCs) in vitro

and to study the effect of CD147 on the synthesis and secretion of matrix metalloproteinases-2 and 9 (MMP-2 and MMP-9) during the differentiation and maturation of OCs in vitro. Method Human peripheral blood mononuclear cells (PBMCs) were obtained from healthy adults by attachment culture. Macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor-kappa Β ligand (RANKL) were used to induce the differentiation of PBMCs into OCs. PBMCs were divided into a control group (RANKL+M-CSF) and a protein induction group (RANKL+M-CSF+CD147). The differentiation and maturation of OCs were identified by tartrate-resistant acid phosphatase (TRAP) staining and bone resorption test. The mRNA expression levels of CD147

MMP-2 and MMP-9 in osteoclast precursor cells (OPCs) were detected by real-time PCR. The protein expression levels of MMP-2 and MMP-9 in the cell supernatant were detected by ELISA. Results OC-like cells were detected and showed a bone resorption function in TRAP staining and bone resorption test. The mRNA expression levels of CD147 (1.344±0.207 and 1.816±0.222)

MMP-2 (1.187±0.077 and 1.437±0.231) and MMP-9 (1.128±0.107 and 2.353±0.679) in the protein induction group at 24 h and 48 h were higher than those in the control group (P0.05)

and the mRNA expression levels of MMP-2 and MMP-9 were positively correlated with those of CD147 (r=0.818

P0.01; r=0.758

P0.01) The protein expression levels of MMP-2 (386.71±40.66 μg/L and 367.35±20.72 μg/L) and MMP-9 (1 177.78±66.42 μg/L and 1 514.37±68.56 μg/L) in the protein induction group at 24 h and 48 h were higher than those in the control group (P0.05). The protein expression levels of MMP-2 in the protein induction group showed no significant difference at 24 h and 48 h (t=1.19，P0.0.05)

but the protein expression levels of MMP-9 at 48 h were higher than that at 24 h (t=7.589，P0.05). Conclusion Exogenous CD147 protein can promote the synthesis and secretion of MMP-9 and MMP-2 during the differentiation and maturation of OCs. However

the secretion increase of MMP-2 is not time-dependent.

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