Correlation of DNMT1 expression with GSTP1 and APC methylation and its clinical significance

张伟杰; 马志俊; 陆士新; 王瑞林; 赵培荣; 王留兴

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Correlation of DNMT1 expression with GSTP1 and APC methylation and its clinical significance

更新时间：2026-01-09

- Correlation of DNMT1 expression with GSTP1 and APC methylation and its clinical significance
- Journal of Army Medical University Vol. 33, Issue 15, Pages: 1602-1605(2011)
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  CLC： R737.25;R394.3;R730.23
- Published：2011
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张伟杰,马志俊,陆士新,王瑞林,赵培荣,王留兴.前列腺癌组织中DNMT1的表达与GSTP1、APC甲基化状态的关系及临床意义[J].陆军军医大学学报(原第三军医大学学报),2011,33(15):1602-1605.

　Zhang Weijie,Ma Zhijun,Lu Shixin,Wang Ruilin,Zhao Peirong,Wang Liuxing.Correlation of DNMT1 expression with GSTP1 and APC methylation and its clinical significance[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(15):1602-1605.
张伟杰,马志俊,陆士新,王瑞林,赵培荣,王留兴.前列腺癌组织中DNMT1的表达与GSTP1、APC甲基化状态的关系及临床意义[J].陆军军医大学学报(原第三军医大学学报),2011,33(15):1602-1605. DOI：

　Zhang Weijie,Ma Zhijun,Lu Shixin,Wang Ruilin,Zhao Peirong,Wang Liuxing.Correlation of DNMT1 expression with GSTP1 and APC methylation and its clinical significance[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(15):1602-1605. DOI：

摘要

目的探讨前列腺癌组织中DNA甲基转移酶1（DNA methyltransferase 1

DNMT1）的表达与谷胱甘肽S转移酶P1（glutathione S transferase P1

GSTP1）、结肠腺瘤性息肉病（adenomatous polyposis coli

APC）甲基化状态的关系及临床意义。方法采用RT-PCR的方法检测56例前列腺癌（prostate cancer，PCa）组织和10例前列腺增生（benign prostatic hyperplasia

BPH）组织中DNMT1和GSTP1、APC的mRNA表达，采用甲基化特异性PCR（MS-PCR）的方法检测GSTP1、APC的启动子区CpG岛甲基化状态。结果大多数PCa组织中DNMT1 mRNA高表达（在低分化癌中阳性率为90.0%）

而GSTP1和APC低表达（在低分化癌中阳性率分别为23.3%和33.3%），BPH组织中DNMT1 mRNA低表达（40.0%），而GSTP1和APC高表达（分别为90.0%和80.0%），低分化癌与BPH相比，差异有统计学意义（P0.05）；大多数PCa组织中GSTP1、APC启动子区CpG岛呈高甲基化状态（在低分化癌中阳性率分别为83.3%和73.3%），而BPH组织中呈低甲基化（阳性率分别为10.0%和20.0%）

低分化癌与BPH相比，差异有统计学意义（P0.05）。结论 PCa组织中GSTP1、APC失活的机制可能与DNMT1的高表达导致其启动子区CpG岛异常高甲基化有关。

Abstract

Objective To investigate the correlation of expression of DNA methyltransferase 1 (DNMT1) and the methylation status of glutathione S transferase P1 (GSTP1) and adenomatous polyposis coli (APC) promoter CpG island in prostate cancer and its clinical significance. Methods RT-PCR method was used to detect the expression of DNMT1

GSTP1

and APC in 56 cases of prostate cancer and 10 cases of benign prostatic hyperplasia. Mutagenivcally separated-PCR (MS-PCR) was used to detect the methylation status of CpG island of promoter of GSTP1 and APC. Results DNMT1 mRNA was highly expressed in most of the prostate cancer tissue (the positive rate in the poorly-differentiated cancer tissues was 90.0%)

but mildly expressed in benign prostatic hyperplasia (the positive rate was 40.0%). GSTP1 and APC mRNA was slightly expressed in most of the prostate cancer tissue (the positive rate of poor-differentiation was 23.3% and 33.3% respectively

P0.05)

but strongly expressed in most benign prostatic hyperplasia tissue (the positive rate was 90.0% and 80.0% respectively). GSTP1 and APC were in hypermethylated status in most prostate cancer tissues (the positive rate of poor-differentiation was 83.3% and 73.3% respectively)

but in hypomethylated status (or demethylation) in benign prostatic hyperplasia (the positive rate was 10.0% and 20.0% respectively

P0.05). Conclusion Silence of GSTP and APC in prostate cancer may be related with hypermethylation of DNMT1 promoter CpG island due to its high expression.

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