cFLIP antisense oligonucleotide inhibits proliferation in human colon carcinoma cell line SW620

文又武; 朱代华; 杨函; 梁光熙

您当前的位置：

首页 >

文章列表页 >

cFLIP antisense oligonucleotide inhibits proliferation in human colon carcinoma cell line SW620

更新时间：2026-01-09

- cFLIP antisense oligonucleotide inhibits proliferation in human colon carcinoma cell line SW620
- Journal of Army Medical University Vol. 33, Issue 14, Pages: 1488-1491(2011)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R735.35;R73-362;R730.23
- Published：2011
- 稿件说明：
移动端阅览
文又武,朱代华,杨函,梁光熙.cFLIP反义寡核苷酸抑制结肠癌细胞SW620的增殖[J].陆军军医大学学报(原第三军医大学学报),2011,33(14):1488-1491.

　Wen Youwu,Zhu Daihua,Yang Han,Liang Guangxi.cFLIP antisense oligonucleotide inhibits proliferation in human colon carcinoma cell line SW620[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(14):1488-1491.
文又武,朱代华,杨函,梁光熙.cFLIP反义寡核苷酸抑制结肠癌细胞SW620的增殖[J].陆军军医大学学报(原第三军医大学学报),2011,33(14):1488-1491. DOI：

　Wen Youwu,Zhu Daihua,Yang Han,Liang Guangxi.cFLIP antisense oligonucleotide inhibits proliferation in human colon carcinoma cell line SW620[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(14):1488-1491. DOI：

摘要

目的研究cFLIP反义寡核苷酸(cFLIP-asODN)对人结肠癌细胞株SW620细胞增殖的影响。方法利用脂质体将cFLIP-asODN转染入SW620细胞，并设空载体+cFLIP-asODN转染组、脂质体+无意义ODN转染组和空白对照组作为对照。荧光倒置显微镜检测复合物对细胞的转染效率；荧光实时定量RT-PCR和Western blot检测转染后细胞中cFLIP mRNA和蛋白的表达水平的变化；MTT法分析复合物对SW620细胞增殖的影响；流式细胞术分析转染后细胞凋亡情况。结果与空载体+cFLIP-asODN转染组、脂质体+无意义ODN转染组和空白对照组相比，脂质体递送cFLIP-asODN进入细胞后，SW620细胞的增殖受到明显抑制(P0.05)；转染cFLIP-asODN后，SW620细胞中的cFLIP mRNA和蛋白的表达水平降低(P0.05)；且细胞凋亡率(29.5±2.4)%也明显高于空载体+cFLIP-asODN转染组(5.8±0.7)%、脂质体+无意义ODN转染组(5.5±0.8)%和空白对照组(5.2±0.5)%(P0.05)。结论通过在体外转染cFLIP-asODN可有效抑制结肠癌细胞株SW620的增殖。

Abstract

Objective To investigate the inhibitory effect of cFLIP antisense oligonucleotide (cFLIP-asODN) on the proliferation of human colon carcinoma cell line SW620. Methods Human colon carcinoma cell line SW620 was transfected with cFLIP-asODN by liposome Lipofectamine

2000. Besides

a cFLIP-asODN group

a liposome+nonsense ODN (NSODN) group and a blank control group were constructed as the control groups. Inverted fluorescence microscope was used to detect the transfection efficiency of the complexes. Real-time

quantitative RT-PCR and Western blotting were used to detect the expression level changes of cFLIP mRNA and protein in SW620 cell after transfection. MTT assay was used to analyze the effects of the complexes on the proliferation of SW620 cell. Flow cytometry was used to determine the apoptosis of SW620 cell after transfection. Results The proliferation of SW620 cell transfected with cFLIP-asODN delivered by liposome Lipofectamine

2000 was significantly inhibited (P0.05)

as compared with the three control groups. The cFLIP mRNA and protein expression levels of transfected SW620 cell were significantly lower than those of non-transfected SW620 cell. The apoptosis of SW620 cell in the liposome+cFLIP-asODN group was significantly higher than those in the three control groups [(29.5±2.4)% vs (5.8±0.7)%

(5.5±0.8)% and (5.2±0.5)%; P0.05

]

. Conclusion In vitro liposome-mediated transfection with cFLIP-asODN can significantly inhibit the proliferation of human colon carcinoma cell line SW620.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of SOX15 gene on proliferation, apoptosis, migration and invasion of colorectal cancer Caco2 cells

Mechanism of suppression on proliferation of human colon carcinoma cell line HT29 by oxaliplatin

Expression of miR-616 in osteosarcoma and its role in proliferation, apoptosis, migration and invasion of tumor cells

Regulation and mechanism of Gm49394 on islet-β cell apoptosis

Celastrol exerts anti-ER+ breast cancer by activating endoplasmic reticulum stress to induce apoptosis

Related Author

王双

陈欣

姜政

向征

张才全

汤为学

FU Wanlei

HAO Xianglin

Related Institution

Department of Pathology， Second Affiliated Hospital， Army Medical University （Third Military Medical University）

Department of Immunology, Faculty of Pharmacy and Laboratory Medicine， Army Medical University （Third Military Medical University）

Department of Laboratory Animal Science， College of Basic Medical Sciences, Faculty of Pharmacy and Laboratory Medicine， Army Medical University （Third Military Medical University）

Department of Pharmaceutical Analysis, Faculty of Pharmacy and Laboratory Medicine， Army Medical University （Third Military Medical University）

Department of Thoracic Surgery, Institute of Surgery Research, Daping Hospital, Third Military Medical University

⁰