Effects of EXf, a fragment of exendin-4, on H<sub>2</sub>O<sub>2</sub>-induced apoptosis in pancreatic islet β-cells line INS-1

董瓅瑾; 金鑫; 高欣; 李文潮; 袁强; 邓靖; 宋光明

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Effects of EXf, a fragment of exendin-4, on H₂O₂-induced apoptosis in pancreatic islet β-cells line INS-1

更新时间：2026-01-09

- Effects of EXf, a fragment of exendin-4, on H₂O₂-induced apoptosis in pancreatic islet β-cells line INS-1
- Journal of Army Medical University Vol. 33, Issue 11, Pages: 1141-1143(2011)
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  CLC： R977.15;R322.57;R587.105
- Published：2011
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董瓅瑾,金鑫,高欣,李文潮,袁强,邓靖,宋光明.重组胰生定多肽对H2O2诱导的INS-1胰岛β细胞凋亡的抑制作用[J].陆军军医大学学报(原第三军医大学学报),2011,33(11):1141-1143.

　Dong Lijin,Jin Xin,Gao Xin,Li Wenchao,Yuan Qiang,Deng Jing,Song Guangming.Effects of EXf, a fragment of exendin-4, on H2O2-induced apoptosis in pancreatic islet β-cells line INS-1[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(11):1141-1143.
董瓅瑾,金鑫,高欣,李文潮,袁强,邓靖,宋光明.重组胰生定多肽对H2O2诱导的INS-1胰岛β细胞凋亡的抑制作用[J].陆军军医大学学报(原第三军医大学学报),2011,33(11):1141-1143. DOI：

　Dong Lijin,Jin Xin,Gao Xin,Li Wenchao,Yuan Qiang,Deng Jing,Song Guangming.Effects of EXf, a fragment of exendin-4, on H2O2-induced apoptosis in pancreatic islet β-cells line INS-1[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(11):1141-1143. DOI：

摘要

目的观察重组胰生定多肽（EXf）对H

诱导的INS-1胰岛β细胞凋亡的影响。方法将INS-1细胞分成5组：空白对照组、阴性对照组、阳性对照组(EX-4)、给药组(EXf，终浓度分别为10、100 nmol/L)，每组至少6孔。除空白对照组外，均给予终浓度为100 μmol/L H

诱导INS-1胰岛β细胞凋亡模型。通过MTT检测法、Hoechest 33342染色法、DNA ladder检测法，观察EXf 对H

诱导INS-1胰岛β细胞细胞存活率、形态学变化和凋亡的影响。结果 MTT实验结果显示与模型对照组相比，EXf能使H

处理的INS-1胰岛β细胞存活率明显增加(P0.01)，并呈剂量依赖性。Hoechst 33342染色检测显示给药组细胞凋亡数量明显减少，正常细胞数量明显增加。EXf能明显抑制DNA ladder的出现，且呈一定量效关系。结论 EXf对H

诱导的INS-1胰岛β细胞的凋亡具有明显的保护作用。

Abstract

Objective To evaluate the effects of EXf

a fragment of exendin-4 (EX-4)

on the H

-induced apoptosis of pancreatic islet β-cell line INS-1. Methods INS-1 cells were divided into five groups

i.e.

a blank control group

a negative control group

a positive control group (EX-4)

and two EXf treatment groups (with EXf final concentration 10 and 100 nmol/L separately). Each group included at least 6 wells of cells. The abo

ve groups (except for the blank control group) were exposed to 100 μmol/L H

to induce INS-1 cell apoptosis. EX-4 and EXf with different concentrations were also given simultaneously in the positive control group and the EXf treatment groups

respectively. The effects of EXf on the viability

morphological change and apoptosis were observed using MTT assay

Hoechest 33342 staining and DNA ladder assay

respectively. Results Compared with the negative control group

the EXf treatment groups had significantly increased viability of INS-1 cells (P0.01) as proven by MTT assay

showing a positive EXf dose-dependent manner. The EXf treatment groups also had an obviously decreased apoptotic cell number and an obviously increased normal cell number as proven by Hoechest 33342 staining. EXf could remarkably inhibit the occurrence of DNA ladder with a dose-effect manner. Conclusion EXf can remarkably inhibit the H

-induced apoptosis in pancreatic islet β-cell line INS-1.

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Effects of EXf, a fragment of exendin-4, on H2O2-induced apoptosis in pancreatic islet β-cells line INS-1

DOI：

摘要

Abstract

关键词

Keywords

references

Effects of EXf, a fragment of exendin-4, on H₂O₂-induced apoptosis in pancreatic islet β-cells line INS-1