Hsa-miR-193b suppresses invasion of human endometrium adenocarcinoma HEC-1B cells

李荣; 陈雪梅; 何俊琳; 丁裕斌; 杨德辉; 许皓; 郑安舜; 刘学庆

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Hsa-miR-193b suppresses invasion of human endometrium adenocarcinoma HEC-1B cells

更新时间：2026-01-09

- Hsa-miR-193b suppresses invasion of human endometrium adenocarcinoma HEC-1B cells
- Journal of Army Medical University Vol. 33, Issue 15, Pages: 1594-1597(2011)
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  CLC： R73-362;R737.33;R394.2
- Published：2011
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李荣,陈雪梅,何俊琳,丁裕斌,杨德辉,许皓,郑安舜,刘学庆.Hsa-miR-193b抑制人子宫内膜腺癌细胞株HEC-1B侵袭能力[J].陆军军医大学学报(原第三军医大学学报),2011,33(15):1594-1597.

　Li Rong,Chen Xuemei,He Junlin,Ding Yubin,Yang Dehui,Xu Hao,Zheng Anshun,Liu Xueqing.Hsa-miR-193b suppresses invasion of human endometrium adenocarcinoma HEC-1B cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(15):1594-1597.
李荣,陈雪梅,何俊琳,丁裕斌,杨德辉,许皓,郑安舜,刘学庆.Hsa-miR-193b抑制人子宫内膜腺癌细胞株HEC-1B侵袭能力[J].陆军军医大学学报(原第三军医大学学报),2011,33(15):1594-1597. DOI：

　Li Rong,Chen Xuemei,He Junlin,Ding Yubin,Yang Dehui,Xu Hao,Zheng Anshun,Liu Xueqing.Hsa-miR-193b suppresses invasion of human endometrium adenocarcinoma HEC-1B cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(15):1594-1597. DOI：

摘要

目的探讨Hsa-miR-193b对人子宫内膜腺癌细胞系HEC-1B侵袭的影响及其相关机制。方法将Hsa-miR-193b模拟物(mimics)、Hsa-miR-193b抑制剂(inhibitor)转染HEC-1B细胞，Real-time PCR证实转染是否成功；Transwell 小室法检测转染Hsa-miR-193b mimics后HEC-1B细胞的侵袭能力；通过miRGen、Targetscan、Pictar数据库筛查Hsa-miR-193b指向与侵袭相关的靶基因；Real-time PCR检测转染Hsa-miR-193b mimics转染组和对照组mRNA水平；Western blot检测各组GRB7蛋白表达的水平。结果 ①Hsa-miR-193b mimics、Hsa-miR-193b inhibitor转染HEC-1B细胞成功，两转染组Hsa-miR-193b表达水平与未转染组比较差异有统计学意义(P0.05)。②转染Hsa-miR-193b mimics组与对照组比较，肿瘤细胞的侵袭能力明显降低(P0.05)。③转染后，靶基因GRB7蛋白Hsa-miR-193b mimics组表达下降(P0.05)、Hsa-miR-193b inhibitor组表达增加(P0.05)，且各组GRB7 mRNA水平无显著变化。结论在HEC-1B中转染Hsa-miR-193b mimics能下调GRB7蛋白表达，并抑制HEC-1B细胞的侵袭。

Abstract

Objective To investigate the influence of Has-miR-193b on the invasion of human endometrium adenocarcinoma HEC-1B cells. Methods Validated Has-miR-193b mimics and inhibitors were transfected into endometrial carcinoma HEC-1B cells. Real-time PCR was used to confirm the efficiency of transfection. Invasiveness of transfected and non-transfected HEC-1B cells was detected by matrix metrigel invasion assay. miRGen

Targetscan

and Pictar database was used to find target genes of Has-miR-193b. Western blotting was used to measure GRB7 protein expression in the cells. Results Hsa-miR-193b expression in HEC-1B cells was significantly change after mimics and inhibitor transfection (P0.05). Has-miR-193b mimics transfection resulted in decrease of invasiveness in HEC-1B cells (P0.05)

and the decrease of GRB7 protein expression (P0.05). While the expression was increased after transfected with Has-miR-193b inhibitor. But no obvious change was found in the GRB7 mRNA expression in nontransfected

mimics-transfected and inhibitor-transfected HEC-1B cells. Conclusion Transfection with Has-miR-193b mimics effectively down-regulates GRB7 expression

and suppresses invasiveness in HEC-1B cells.

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