Anti-survivin oligonucleotides suppresses growth, invasion and migration in human gastric cancer BGC-823 cells

李勇; 范立侨; 赵群; 孙艳; 焦志凯; 张志栋; 檀碧波

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Anti-survivin oligonucleotides suppresses growth, invasion and migration in human gastric cancer BGC-823 cells

更新时间：2026-01-09

- Anti-survivin oligonucleotides suppresses growth, invasion and migration in human gastric cancer BGC-823 cells
- Journal of Army Medical University Vol. 33, Issue 13, Pages: 1350-1352(2011)
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  CLC： R394.3;R73-362;R735.2
- Published：2011
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李勇,范立侨,赵群,孙艳,焦志凯,张志栋,檀碧波.针对survivin的反义寡核苷酸对人胃癌细胞株BGC-823生长、侵袭、迁移能力的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(13):1350-1352.

　Li Yong,Fan Liqiao,Zhao Qun,Sun Yan,Jiao Zhikai,Zhang Zhidong,Tan Bibo.Anti-survivin oligonucleotides suppresses growth, invasion and migration in human gastric cancer BGC-823 cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(13):1350-1352.
李勇,范立侨,赵群,孙艳,焦志凯,张志栋,檀碧波.针对survivin的反义寡核苷酸对人胃癌细胞株BGC-823生长、侵袭、迁移能力的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(13):1350-1352. DOI：

　Li Yong,Fan Liqiao,Zhao Qun,Sun Yan,Jiao Zhikai,Zhang Zhidong,Tan Bibo.Anti-survivin oligonucleotides suppresses growth, invasion and migration in human gastric cancer BGC-823 cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(13):1350-1352. DOI：

摘要

目的观察生存素（survivin）反义寡核苷酸（anti-survivin oligonucleotides

ASODN）对人胃癌细胞株BGC-823增殖、凋亡、侵袭、迁移能力的影响。方法脂质体介导ASODN或对照SODN转染人胃癌细胞株BGC-823后，RT-PCR方法检测survivin的mRNA表达水平，四氮唑盐（MTT）法检测ASODN或SODN对肿瘤细胞的增殖抑制效果，Transwell小室法检测细胞侵袭和迁移能力的改变，流式细胞术检测细胞周期及凋亡指数的改变。结果 ASODN转染可明显降低survivin 的表达（P0.05），并且ASODN转染具有以浓度及时间依赖的方式抑制BGC-823细胞增殖的作用（P0.05）。用600 nmol/L浓度的ASODN转染BGC-823后，BGC-823细胞迁移和侵袭能力下降（P0.05），细胞凋亡指数（apoptotic index，AI）由(1.62±0.36)增加到(11.31±0.38)（P0.01），而增殖指数（proliferation index，PI）则由(43.80±0.81)下降至(27.77±2.97)（P0.05）。停滞于G

/M期的细胞明显增加（P0.01）。结论下调survivin表达的ASODN可通过抑制胃癌细胞增殖、诱导凋亡、降低胃癌细胞侵袭和迁移能力而发挥抗肿瘤作用。

Abstract

Objective To determine the effects of anti-survivin oligonucleotides (ASODN) on the proliferation

apoptosis

invasion and migration in gastric cancer BGC-823 cells. Methods After ASODN or SODN was introduced by Lipofectamine

2000 transfection reagent into BGC-823 cells

the mRNA expression of survivin

was detected by RT-PCR

cell proliferation was measured by 4-methyl-tetrazolium (MTT) assay

cell

invasion and migration were assayed by Transwell chamber tests

and cell cycle and apoptosis index (AI) was detected by flow cytometry. Results ASODN transfection resulted in a marked decrease of survivin expression (P0.05). And the cell proliferation was inhibited by ASODN transfection in a dose- and time-dependent manner (both P0.05). Both cell invasion and migration activities of BGC-823 were decreased (P0.05) after the transfection of 600 nmol/L ASODN. AI of ASODN transfection group was increased significantly when compared with that of control group (11.31±0.38 vs 1.62±0.36

P0.01)

and proliferation Index (PI) was decreased significantly in ASODN transfection group than in control group (27.77±2.97 vs 43.80±0.81

P0.05). Cells in G

/M stage were increased in ASODN transfection group compared with control group (P0.01). Conclusion Down-regulation of survivin by ASODN inhibits the invasion and migration of BGC-823 cells through suppressing cell proliferation and improving cell apoptosis.

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