M. tuberculosis antigen-specific CD8<sup>+</sup> memory T cell subsets in patients with active pulmonary tuberculosis

蒋静; 王仙园; 邓少丽; 王心静; 曹志红; 刘艳华; 程小星

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M. tuberculosis antigen-specific CD8⁺ memory T cell subsets in patients with active pulmonary tuberculosis

更新时间：2026-01-09

- M. tuberculosis antigen-specific CD8⁺ memory T cell subsets in patients with active pulmonary tuberculosis
- Journal of Army Medical University Vol. 33, Issue 9, Pages: 905-907(2011)
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  CLC： R446.6;R392.12;R521.04
- Published：2011
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蒋静,王仙园,邓少丽,王心静,曹志红,刘艳华,程小星.结核患者抗原特异性CD8+记忆T细胞的检测分析[J].陆军军医大学学报(原第三军医大学学报),2011,33(09):905-907.

　Jiang Jing,Wang Xianyuan,Deng Shaoli,Wang Xinjing,Cao Zhihong,Liu Yanhua,Cheng Xiaoxing.M. tuberculosis antigen-specific CD8+ memory T cell subsets in patients with active pulmonary tuberculosis[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(09):905-907.
蒋静,王仙园,邓少丽,王心静,曹志红,刘艳华,程小星.结核患者抗原特异性CD8+记忆T细胞的检测分析[J].陆军军医大学学报(原第三军医大学学报),2011,33(09):905-907. DOI：

　Jiang Jing,Wang Xianyuan,Deng Shaoli,Wang Xinjing,Cao Zhihong,Liu Yanhua,Cheng Xiaoxing.M. tuberculosis antigen-specific CD8+ memory T cell subsets in patients with active pulmonary tuberculosis[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(09):905-907. DOI：

摘要

目的研究活动期肺结核患者和潜伏感染者CD8

记忆T细胞的产生及分布特性。方法 24例活动性肺结核患者均为确诊的住院患者，22例潜伏感染者经ELISPOT检查确定。取外周血分离获取外周血单个核细胞（peripheral blood mononuclear cells，PBMCs），以结核分枝杆菌抗原ESAT-6刺激，通过CD8、CD107a、CCR7、CD45RA抗体共染色，采用流式细胞术分析。结果建立了基于CD107a为分子标识的结核抗原特异性CD8

T细胞检测方法。对24例活动性肺结核患者及22例潜伏感染者对比分析发现，与潜伏感染者相比，肺结核患者CD45RA阳性CD8

记忆T细胞T

EMRA

亚群比例显著增加[(59.94±3.87)% vs (46.65±2.88)%，P=0.020 3

]

，CD8

效应记忆T细胞T

亚群分布频率显著降低[（38.14±3.87）% vs （52.34±2.95）%，P=0.014 2

]

。结论肺结核患者与潜伏感染者抗原特异性CD8

记忆T淋巴细胞亚群的比例存在明显差异，可能与体内抗原刺激的程度及特异细胞免疫应答反应相关。

Abstract

Objective To determine the production and frequencies of M. tuberculosis antigen-specific CD8

memory T cells from patients with pulmonary tuberculosis and individuals with latent infection. Methods A total of 24 patients with pulmonary tuberculosis who were hospitalized in No. 309 Hospital from November to December 2009 and 22 individuals with latent infection who were outpatients during same time period were recruited in the study. The

ir PBMCs were stimulated with M. tuberculosis antigen ESAT-6. The CD8

memory T cell subsets were analyzed by CD8

CD107a

CCR7 and CD45RA staining and flow cytometry. Results CD8

antigen-specific T cells were detected based on surface CD107a expression following antigen stimulation. Analysis of antigen-specific CD8 memory T cells from 24 patients with active pulmonary tuberculosis and 22 individuals with latent infection showed that the frequencies of the CD45RA-positive CD8

memory T cell (T

EMRA

) subset were significantly increased [(59.94±3.87)% vs (46.65±2.88)%

P=0.020 3

]

and the frequencies of CD8

memory T cell (T

) subset were significantly decreased [(38.14±3.87)% vs (52.34±2.95)%

P=0.014 2

]

in patients with active pulmonary tuberculosis

compared with individuals with latent infection. Conclusion There are significant difference in frequencies of antigen-specific CD8

EMRA

and T

among people with active pulmonary tuberculosis and latent infection

which may be correlated with antigen load in vivo and antigen specific cellular immune responses.

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Related Institution

Institute of Cancer， Xinqiao Hospital， Army Medical University （Third Military Medical University）

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M. tuberculosis antigen-specific CD8+ memory T cell subsets in patients with active pulmonary tuberculosis

DOI：

摘要

Abstract

关键词

Keywords

references

M. tuberculosis antigen-specific CD8⁺ memory T cell subsets in patients with active pulmonary tuberculosis