Role of liver X receptor in expression of B lymphocyte stimulator in Raji cells

黄艳; 黄刚; 胡长江; 曾益军; 许志臻; 陈岺曦; 何凤田; 宋方洲

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Role of liver X receptor in expression of B lymphocyte stimulator in Raji cells

更新时间：2026-01-09

- Role of liver X receptor in expression of B lymphocyte stimulator in Raji cells
- Journal of Army Medical University Vol. 33, Issue 13, Pages: 1342-1345(2011)
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  CLC： R394;R73-362;R730.263
- Published：2011
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黄艳,黄刚,胡长江,曾益军,许志臻,陈岺曦,何凤田,宋方洲.GW3965在Raji细胞中对BLyS表达的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(13):1342-1345.

　Huang Yan,Huang Gang,Hu Changjiang,Zeng Yijun,Xu Zhizhen,Chen Lingxi,He Fengtian,Song Fangzhou.Role of liver X receptor in expression of B lymphocyte stimulator in Raji cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(13):1342-1345.
黄艳,黄刚,胡长江,曾益军,许志臻,陈岺曦,何凤田,宋方洲.GW3965在Raji细胞中对BLyS表达的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(13):1342-1345. DOI：

　Huang Yan,Huang Gang,Hu Changjiang,Zeng Yijun,Xu Zhizhen,Chen Lingxi,He Fengtian,Song Fangzhou.Role of liver X receptor in expression of B lymphocyte stimulator in Raji cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(13):1342-1345. DOI：

摘要

目的探讨肝X受体（liver X receptors

LXRs）的特异性激动剂GW3965在Raji细胞中对B淋巴细胞刺激因子(B lymphocyte stimulator

BLyS)表达的影响。方法 MTT法检测不同浓度的GW3965（浓度分别为0.5、5、10 μmol/L）对Raji细胞活性的影响，观察时间为加入GW3965共孵育后24 h。用LXR的特异性激动剂GW3965刺激人B细胞淋巴瘤株Raji细胞，经 RT-PCR 检测 LXR特异性靶基因三磷酸腺苷结合盒A1 (ATP-binding cassette

ABCA1) mRNA的表达;经 RT-PCR和 Western blot 检测BLyS的表达。结果对照组1 ‰DMSO D（492）为（0.632±0.055），0.5、5 μmol/L和10 μmol/L的GW3965 D（492）为（0.609±0.073）、（0.612±0.052）和（0.628±0.055）。LXR的特异性配体GW3965对细胞活性无影响（P0.05）。GW3965作用于Raji细胞后

ABCA1 mRNA 表达剂量依赖性上调

LXR活化后可在转录和翻译水平剂量依赖性下调抑制BLyS的表达。结论 LXR在Raji细胞中可抑制BLyS的表达。

Abstract

Objective To determine the effect of liver X receptor (LXR) specific agonist

GW3965 on the expression of B lymphocyte stimulator (BLyS) in human B-cell lymphoma Raji cells. Methods Cell viability of Raji cells exposure to GW3965 at concentrations of 0.5

5 and 10 μmol/L for 24 h was detected by MTT assay. Then the expression of ATP-binding cassette (ABCA1

the specific target gene of LXR) mRNA and LXR mRNA was detected by RT-PCR and the expression of BLyS at mRNA and protein levels were measured by RT-PCR and Western blotting. Results The level of 1‰ DMSO OD(492 nm) was（0.632±0.055）

and 0.5

5 and 10 μmol/L GW3965 resulted in the levels of OD(492nm) were （0.609±0.073）

（0.612±0.052） and（0.628±0.055） respectively

indicating that GW3965 had no effect on the viability of Raji cells (P0.05). Exposure to 0.5 and 5 μmol/L GW3965 results in an increase in the level of ABCA1 mRNA in a dose-dependent manner

suggesting LXR was functional in Raji cells. The expression of BLyS were down-regulated in both mRNA and protein levels after LXR activation. Conclusion LXR decreases the expression of BLyS in Raji cells.

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