Determination of cyclin D<sub>2</sub> promoter region in response to trichostatin A treatment

郭志义; 郝小惠; 田炜; 谭菲菲; 姜雪莲; 李潇婷; 胡倩倩; 杨方

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Determination of cyclin D₂ promoter region in response to trichostatin A treatment

更新时间：2026-01-09

- Determination of cyclin D₂ promoter region in response to trichostatin A treatment
- Journal of Army Medical University Vol. 33, Issue 14, Pages: 1458-1461(2011)
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  CLC： R979.1;R966;R394-33
- Published：2011
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郭志义,郝小惠,田炜,谭菲菲,姜雪莲,李潇婷,胡倩倩,杨方.细胞周期素D2启动子曲古菌素A效应区域的研究[J].陆军军医大学学报(原第三军医大学学报),2011,33(14):1458-1461.

　Guo Zhiyi,Hao Xiaohui,Tian Wei,Tan Feifei,Jiang Xuelian,Li Xiaoting,Hu Qianqian,Yang Fang.Determination of cyclin D2 promoter region in response to trichostatin A treatment[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(14):1458-1461.
郭志义,郝小惠,田炜,谭菲菲,姜雪莲,李潇婷,胡倩倩,杨方.细胞周期素D2启动子曲古菌素A效应区域的研究[J].陆军军医大学学报(原第三军医大学学报),2011,33(14):1458-1461. DOI：

　Guo Zhiyi,Hao Xiaohui,Tian Wei,Tan Feifei,Jiang Xuelian,Li Xiaoting,Hu Qianqian,Yang Fang.Determination of cyclin D2 promoter region in response to trichostatin A treatment[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(14):1458-1461. DOI：

摘要

目的研究在曲古菌素A(trichostatin A

TSA)处理条件下细胞周期素D

的调控机制。方法细胞周期素D

的mRNA水平通过RT-PCR方法检测。细胞周期素D

的启动子片段以A549细胞基因组DNA为模板，通过高保真PCR扩增获得，并克隆到pGL3-BASIC载体中；并以此为基础通过高保真PCR扩增出不同长度的启动子片段。启动子相对活性通过萤火虫酶报告基因方法确定。结果在TSA处理条件下，细胞周期素D

的mRNA水平上升。共构建4个以不同长度细胞周期素D

启动子片段驱动的报告基因质粒，分别命名为pGL3-1816CD2-LUC、pGL3-1358CD2-LUC、pGL3-720CD2-LUC和pGL3-524CD2-LUC。在TSA处理条件下，启动子活性的变化在-524 bp删切质粒时消失。结论 TSA条件下细胞周期素D

mRNA水平上升是启动子依赖的；细胞周期素D

启动子的TSA效应区域在-720~-524 bp之间。

Abstract

Objective To explore the regulation mechanism of cyclin D

under trichostatin A (TSA) treatment. Methods The mRNA level of cyclin D

was detected by RT-PCR. Cyclin D

promoter fragment was amplified by high-fidelity PCR with A549 cell genome DNA as a template

and was then inserted into plasmid vector pGL3-BASIC. Truncated promoter fragments were amplified based on the recombinant plasmid. The relative activity of the promoter fragments was evaluated with the firefly luciferase reporter gene method. Re

sults The mRNA level of cyclin D

increased under TSA treatment. Four plasmids carrying the truncated cyclin D

promoter fragments to control reporter gene expression were constructed and named pGL3-1816CD2-LUC

pGL3-1358CD2-LUC

pGL3-720CD2-LUC and pGL3-524CD2-LUC. Under TSA treatment

the promoter activity diminished when the promoter fragment was truncated to 524 bp. Conclusion The mRNA level increase of cyclin D

depends on the promoter under TSA treatment. The TSA response region in cyclin D

promoter is located in the range from 720 bp to 524 bp.

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Determination of cyclin D2 promoter region in response to trichostatin A treatment

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摘要

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Determination of cyclin D₂ promoter region in response to trichostatin A treatment