Influence of X-box binding protein 1 on expression of inflammatory factor in lipopolysacharide-induced Kupffer cells

李铁军; 刘作金; 李生伟; 刘长安; 屈谦; 薛强; 龚建平

您当前的位置：

首页 >

文章列表页 >

Influence of X-box binding protein 1 on expression of inflammatory factor in lipopolysacharide-induced Kupffer cells

更新时间：2026-01-09

- Influence of X-box binding protein 1 on expression of inflammatory factor in lipopolysacharide-induced Kupffer cells
- Journal of Army Medical University Vol. 33, Issue 14, Pages: 1455-1457(2011)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R322.47;R341;R329.28
- Published：2011
- 稿件说明：
移动端阅览
李铁军,刘作金,李生伟,刘长安,屈谦,薛强,龚建平.X盒结合蛋白1对脂多糖处理的Kupffer细胞炎性因子表达的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(14):1455-1457.

　Li Tiejun,Liu Zuojin,Li Shengwei,Liu Changan,Qu Qian,Xue Qiang,Gong Jianping.Influence of X-box binding protein 1 on expression of inflammatory factor in lipopolysacharide-induced Kupffer cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(14):1455-1457.
李铁军,刘作金,李生伟,刘长安,屈谦,薛强,龚建平.X盒结合蛋白1对脂多糖处理的Kupffer细胞炎性因子表达的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(14):1455-1457. DOI：

　Li Tiejun,Liu Zuojin,Li Shengwei,Liu Changan,Qu Qian,Xue Qiang,Gong Jianping.Influence of X-box binding protein 1 on expression of inflammatory factor in lipopolysacharide-induced Kupffer cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(14):1455-1457. DOI：

摘要

目的探讨X盒结合蛋白1 (X-box binding protein 1，XBP1)对脂多糖(lipopolysaccharide，LPS)处理的Kupffer细胞(Kupffer cells，KCs) TNF-α表达的影响。方法将BALB/c小鼠KCs随机区组法分为质粒组、阴性组和空白组。分别给予XBP1-shRNA、空白质粒转染和空白处理，并给予LPS。免疫细胞化学（SABC法）、Real-time PCR和Western blot法检测各组KCs中XBP1基因及蛋白表达水平；酶联免疫吸附法（ELISA）检测核因子NF-κB活性及培养上清液中TNF-α含量。结果与阴性组、空白组相比，质粒组XBP1及下游因子TNF-α表达明显降低（P0.05）；而阴性组、空白组间比较比较无明显差异（P0.05）。3组间NF-κB活性比较无明显差异（P0.05）。结论 XBP1-shRNA质粒能明显抑制KCs XBP1基因的表达，并通过非NF-κB通路减少其下游因子TNF-α的释放。

Abstract

Objective To explore the influence of X-box binding protein 1 (XBP1) on the expression of inflammatory factor of lipopolysaccharide (LPS)-induced Kupffer cells (KCs). Methods KCs derived from BALB/c mice were randomly divided into an XBP1-shRNA group

a negative control group and a blank control group

which received XBP1-shRNA plasmid transfection

control vector transfection and no treatment

respectively. Then the three groups were all treated with LPS. The mRNA and protein expression of XBP1 in KCs was determined by Real-time PCR

Western blotting and immunohistochemistry. The activity of NF-κB and the density of TNF-α in the supernatant of coculture were assessed by enzyme-linked immunosorbent assay (ELISA). Results The expression of XBP1 and its downstream factor TNF-α was significantly lower than that in the negative control group and blank control group (P0.05). However

there was no significant difference between the two control groups (P0.05). In addition

the activity of NF-κB in the three groups had no significant difference (P0.05). Conclusion XBP1-shRNA plasmid can significantly inhibit the expression of XBP1 gene and reduce the release of its downstream factor TNF-α without changing the activity of NF-κB in KCs.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Naringin improves lipopolysaccharide-induced depression-like behaviors in mice

The role of cathepsin K in cardiac dysfunction in mice with alcoholic cardiomyopathy and its damage mechanism

Chaihu Oral Liquid improves hippocampal neurogenesis and short-term memory defects in adult BTBR autism mice

Alpha-ketoglutarate alleviates cholestatic liver injury in mice

CX3CR1 silencing-mediated macrophage polarization attenuates CCl4 -induced liver fibrosis in mice

Related Author

吴川

孔安琪

谢汝欣

李江辉

蔡云

范晓棠

唐一锋

刘志江

Related Institution

No data

⁰