Objective    To investigate the role of poly(ADP-ribose) glycohydrolase (PARG) inhibition in the regulation of vascular endothelial growth factor C (VEGF-C) expression in colon cancer cell CT26 in mice.     Methods    CT26 cells were divided into an experimental group and a control group. CT26 cells in the experimental group were treated with PARG inhibitor gallotannin (GLTN). The expression levels of PARG

PARP-1

NF-κB and VEGF-C were detected by Western blotting. The VEGF-C mRNA level changes of the two groups were detected by RT-PCR. The VEGF-C protein level changes in the supernatants of cultured CT26 cells in the two groups were measured by enzyme-linked immunosorbent assay (ELISA).     Results    Western blot results showed that the PARG

PARP-1

NF-κB

and VEGF-C protein levels in the experimental group were significantly lower than those in the control group (P0.01). The VEGF-C mRNA level (0.39±0.08) and the VEGF-C protein level (95.86±5.43) pg/ml in the experimental group were significantly lower than that (0.63±0.04) in the control group (P0.01) and that (199.32±0.75) pg/ml in the control group (P0.05).     Conclusion    In mouse colon cancer cell CT26

PARG inhibition dramatically reduces VEGF-C expression by down-regulating PARP-1 and NF-κB expression in the nuclear factor NF-κB signaling pathway. PARG inhibition may have an anti-lymphangiogenc effect and contribute to the prevention of metastatic dissemination via lymph system.