Effect of noggin protein on proliferation and invasion of breast cancer MCF-7 cells

郭丹; 龚建平; 孙治君; 印国兵

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Effect of noggin protein on proliferation and invasion of breast cancer MCF-7 cells

更新时间：2026-01-09

- Effect of noggin protein on proliferation and invasion of breast cancer MCF-7 cells
- Journal of Army Medical University Vol. 33, Issue 12, Pages: 1240-1242(2011)
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  CLC： R341;R73-362;R737.9
- Published：2011
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郭丹,龚建平,孙治君,印国兵.Noggin蛋白对MCF-7细胞增殖、侵袭能力的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(12):1240-1242.

　Guo Dan,Gong Jianping,Sun Zhijun,Yin Guobing.Effect of noggin protein on proliferation and invasion of breast cancer MCF-7 cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(12):1240-1242.
郭丹,龚建平,孙治君,印国兵.Noggin蛋白对MCF-7细胞增殖、侵袭能力的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(12):1240-1242. DOI：

　Guo Dan,Gong Jianping,Sun Zhijun,Yin Guobing.Effect of noggin protein on proliferation and invasion of breast cancer MCF-7 cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(12):1240-1242. DOI：

摘要

目的观察Noggin蛋白对乳腺癌细胞MCF-7增殖、侵袭能力的影响。方法以Noggin重组腺病毒感染乳腺癌细胞株MCF-7，MTT法检测Noggin对MCF-7细胞增殖能力的影响，划痕修复实验及Transwell细胞侵袭实验检测其运动和侵袭能力的改变，Real-time PCR和Western blot检测CXCR4和MMP1基因的表达改变。结果过表达Noggin蛋白的MCF-7细胞增殖能力增强（P0.05）；划痕修复实验提示过表达Noggin蛋白的MCF-7细胞划痕愈合延迟；Transwell细胞侵袭实验提示与空病毒组相比，Noggin组穿膜细胞数显著降低（55±4 vs 25±3

P0.05）。RT-PCR和Western blot结果：过表达Noggin后，MCF-7细胞中CXCR4和MMP1基因表达下调。结论 Noggin蛋白可抑制乳腺癌细胞MCF-7的运动和迁移，可能与下调CXCR4和MMP1基因的表达相关。

Abstract

Objective To observe the effect of noggin protein on proliferation and invasion of breast cancer MCF-7 cells. Methods MCF-7 cells were infected with recombinant noggin adenovirus. Effect of noggin protein on proliferation of MCF-7 breast cancer cells was detected by MTT assay. Changes in movement and invasion of MCF-7 breast cancer cells were examined by cell scratching test and trans-well cell invasion experiment. Expression of MMP-1 and CXCR4 was assayed by real-time PCR and Western blotting. Results The proliferation of MCF-7 cells in over-expressed noggin protein was increased（P0.05）. Scratching test showed that the healing of scratched MCF-7 breast cancer cells was delayed in over-expressed noggin protein. Trans-well cell invasion experiment showed that the number of MCF-7 breast cancer cells that penetrated the cell membrane was significantly less in noggin group than in GFP group (25±3 vs 55±4

P0.05）. Real-time PCR and Western blotting showed that the expression of CXCR4 and MMP1 was down-regulated in MCF-7 breast cancer cells when the noggin protein was over-expressed. Conclusion Noggin protein can inhibit the movement and migration of MCF-7 breast cancer cells by down-regulating the expression of CXCR4 and MMP1.

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