Atorvastatin inhibits CTGF and Cx43 expressions in primarily cultured rat atrial myocytes

张卫泽; 宋振华; 陈永清; 马凌; 李丹丹; 惠玲玲

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Atorvastatin inhibits CTGF and Cx43 expressions in primarily cultured rat atrial myocytes

更新时间：2026-01-09

- Atorvastatin inhibits CTGF and Cx43 expressions in primarily cultured rat atrial myocytes
- Journal of Army Medical University Vol. 33, Issue 7, Pages: 735-737(2011)
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  CLC： R972.2;R322.11;R394.2
- Published：2011
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张卫泽,宋振华,陈永清,马凌,李丹丹,惠玲玲.阿托伐他汀抑制心房肌细胞CTGF及Cx43的表达[J].陆军军医大学学报(原第三军医大学学报),2011,33(07):735-737.

　Zhang Weize,Song Zhenhua,Chen Yongqing,Ma Ling,Li Dandan,Hui Lingling.Atorvastatin inhibits CTGF and Cx43 expressions in primarily cultured rat atrial myocytes[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(07):735-737.
张卫泽,宋振华,陈永清,马凌,李丹丹,惠玲玲.阿托伐他汀抑制心房肌细胞CTGF及Cx43的表达[J].陆军军医大学学报(原第三军医大学学报),2011,33(07):735-737. DOI：

　Zhang Weize,Song Zhenhua,Chen Yongqing,Ma Ling,Li Dandan,Hui Lingling.Atorvastatin inhibits CTGF and Cx43 expressions in primarily cultured rat atrial myocytes[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(07):735-737. DOI：

摘要

目的探讨阿托伐他汀（atorvastatin）对血管紧张素Ⅱ（AngⅡ）诱导的心房肌细胞结缔组织生长因子（connective tissue growth factor，CTGF）及缝隙连接蛋白43(Connexin 43，Cx43)表达的影响。方法 18只雌雄对半1周龄左右Wistar大鼠，用于体外心房肌细胞的分离培养与鉴定。设置6组（n=18）：正常对照组、AngⅡ(终浓度1 μmol/L)组、AngⅡ+二甲基亚砜（DMSO）组、AngⅡ+atorvastatin(0.1、1.0、10 μmol/L)组，作用72 h后RT-PCR分别检测CTGF、TGF-β1 及Cx43的mRNA表达。结果与正常对照组相比，AngⅡ组CTGF、TGF-β1和Cx43 mRNA表达呈显著增加(P0.05)，阿托伐他汀逆转上述变化，10 μmol/L组作用最强（P0.05)，而作为溶剂的DMSO无明显作用（P0.05）。结论阿托伐他汀可能通过抑制AngⅡ诱导的心房肌细胞CTGF mRNA的高表达来减轻心房纤维化，同时可能通过下调Cx43 mRNA的表达来逆转缝隙连接蛋白的重构，最终减低房颤发生率。

Abstract

Objective To investigate the effect of atorvastatin on the expressions of angiotensin Ⅱ-induced atrial myocytes connective tissue growth factor （CTGF）and Connexin 43 (Cx43) in atrial myocytes. Methods A total of 18 Wistar rats of both genders

weighing from 35 to 40 g and aging about 1 week were used for culturing primary atrial myocytes and identification. The cells were divided into 6 groups

normal control

AngⅡ(1 μmol/L)

AngⅡ+dimethyl sulfoxide (DMSO) and AngⅡ+ atorvastatin (0.1

1.0 and 10 μmol/L). In 72 h after treatment

mRNA expressions of CTGF

transforming growth factor-β1（TGF-β1）and Gx43 were measured by reverse transcription-polymerase chain reaction (RT-PCR). Results Compared with normal control group

expressions of CTGF

TGF-β1 and Gx43 were increased significantly in AngⅡ group (P0.05)

but atorvastatin reversed those changes in a dose-dependent manner (P0.05). However

DMSO had no such effect as a solvent（P0.05). Conclusion Atorvastatin maybe attenuate atrial fribosis by inhibiting the increase of mRNA expression of CTGF induced by AngⅡ

reverse the remodeling of connexin by downregulating mRNA expression of Cx43

and thus reduce the incidence rate of atrial fibrillation.

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