pEGFP-N1-DCN suppresses proliferation and invasion of human gastric cancer SGC-7901 cells

袁媛; 王川; 姜政; 王丕龙

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pEGFP-N1-DCN suppresses proliferation and invasion of human gastric cancer SGC-7901 cells

更新时间：2026-01-09

- pEGFP-N1-DCN suppresses proliferation and invasion of human gastric cancer SGC-7901 cells
- Journal of Army Medical University Vol. 33, Issue 7, Pages: 723-727(2011)
- 作者机构：
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  CLC： R730.23;R735.2;R394.3
- Published：2011
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袁媛,王川,姜政,王丕龙.pEGFP-N1-DCN重组质粒抑制胃癌细胞SGC-7901的增殖和侵袭[J].陆军军医大学学报(原第三军医大学学报),2011,33(07):723-727.

　Yuan Yuan,Wang Chuan,Jiang Zheng,Wang Pilong.pEGFP-N1-DCN suppresses proliferation and invasion of human gastric cancer SGC-7901 cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(07):723-727.
袁媛,王川,姜政,王丕龙.pEGFP-N1-DCN重组质粒抑制胃癌细胞SGC-7901的增殖和侵袭[J].陆军军医大学学报(原第三军医大学学报),2011,33(07):723-727. DOI：

　Yuan Yuan,Wang Chuan,Jiang Zheng,Wang Pilong.pEGFP-N1-DCN suppresses proliferation and invasion of human gastric cancer SGC-7901 cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(07):723-727. DOI：

摘要

目的构建 pEGFP-N1-DCN真核表达载体并转染胃癌细胞SGC-7901，观察其对细胞增殖、周期、迁移、侵袭的影响。方法将细胞分为3组，分别为空白对照组、pEGFP-N1组及pEGFP-N1-DCN组，质粒通过脂质体转染SGC-7901，RT-PCR及Western blot方法检测表皮生长因子受体（EGFR）及TGF-β

的表达，MTT法检测细胞增殖，流式细胞仪检测细胞周期，Transwell检测核心蛋白聚糖（DCN）对细胞迁移及侵袭能力的影响。结果 RT-PCR及Western blot检测结果显示

DCN使EGFR及TGF-β

的表达均降低(P0.05)；MTT法检测发现72 h时空白对照组、pEGFP-N1组及pEGFP-N1-DCN组细胞D（490）值分别为（1.190±0.037）、（1.169±0.052）、（0.572±0.021），DCN使细胞增殖受到明显抑制(P0.05)；流式细胞仪分析细胞周期空白对照组、pEGFP-N1组及pEGFP-N1-DCN组细胞G

的细胞数分别为（46.12±0.63）、（46.07±0.82）、（56.36±0.92），DCN使细胞阻滞在G

（P0.05）；Transwell迁移、侵袭实验显示DCN使细胞迁移和侵袭能力均降低（P0.05）。结论 DCN通过抑制肿瘤细胞的增殖、迁移、侵袭等起到抗肿瘤作用。

Abstract

Objective To construct an eukaryotic expression vector encoding decorin (DCN) (pEGFP-N1-DCN) and determine its effect on proliferation

cell cycle

migration and invasion of human gastric cancer cell line SGC-7901 after transfection. Methods SGC-7901 cells were divided into 3 groups

control

pEGFP-N1 group and pEGFP-N1-DCN group. SGC-7901 cells were transfected by correspondin

g plasmids with liposome. The expressions of epidermal growth factor receptor (EGFR) and transforming growth factor-β1 (TGF-β1) were detected by Western blotting and RT-PCR

cell proliferation by MTT assay

cell cycle by flow cytometry and cell migration and invasion by Transwell chamber test. Results RT-PCR and Western blotting results showed that the expressions of EGFR and TGF-β

were decreased in pEGFP-N1-DCN group. MTT assay demonstrated that the value of D（490） of the control group

pEGFP-N1 group and pEGFP-N1-DCN group were 1.190±0.037

1.169±0.052

and 0.572±0.021 respectively

indicating that DCN made cell proliferation inhibited （P0.05）. Flow cytometric analysis showed that cell population at G

to G

stage of these 3 groups were (46.12±0.63)%

(46.07±0.82)%

and (56.36±0.92)%

and the cells in pEGFP-N1-DCN group were arrested at G

to G

stage （P0.05）. Transwell assay showed that DCN inhibits cell migration and invasion（P0.05）. Conclusion DCN plays a role of anti-tumor by inhibiting tumor cell proliferation

migration and invasion.

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