Correlation of low expression of Caveolin-1 and HBV related hepatocellular carcinoma

杨雨晗; 邓缅

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Correlation of low expression of Caveolin-1 and HBV related hepatocellular carcinoma

更新时间：2026-01-09

- Correlation of low expression of Caveolin-1 and HBV related hepatocellular carcinoma
- Journal of Army Medical University Vol. 33, Issue 7, Pages: 715-718(2011)
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  CLC： R394.3;R512.62;R735.7
- Published：2011
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杨雨晗,邓缅.Caveolin-1的低表达与乙型肝炎病毒感染相关肝细胞癌的关系研究[J].陆军军医大学学报(原第三军医大学学报),2011,33(07):715-718.

　Yang Yuhan,Deng Mian.Correlation of low expression of Caveolin-1 and HBV related hepatocellular carcinoma[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(07):715-718.
杨雨晗,邓缅.Caveolin-1的低表达与乙型肝炎病毒感染相关肝细胞癌的关系研究[J].陆军军医大学学报(原第三军医大学学报),2011,33(07):715-718. DOI：

　Yang Yuhan,Deng Mian.Correlation of low expression of Caveolin-1 and HBV related hepatocellular carcinoma[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(07):715-718. DOI：

摘要

目的研究小窝蛋白-1（Caveolin-1）的表达与乙型肝炎病毒（HBV）感染以及相关肝细胞癌（HCC）的关系。方法免疫组化法检测HBV相关HCC中Caveolin-1表达量。构建Caveolin-1重组质粒，用Western blot检测转染了重组质粒的HepG2.2.15细胞中Caveolin-1表达量，并用ELISA法检测转染后培养上清中的HBsAg。结果免疫组化结果显示Caveolin-1在95% HBV相关HCC 组织中表达低于癌旁组织（P0.05）。Caveolin-1重组质粒构建成功并在HepG2.2.15中成功表达。ELISA检测发现用Caveolin-1重组质粒转染HBV感染稳定模型HepG2.2.15细胞48、72 h后，细胞培养上清中的HBsAg浓度相对未转染细胞分别下降了46.2%和48.8%（P0.05）。重组质粒转染HBV感染急性细胞模型48、72 h 后，HBsAg浓度相对未转染细胞分别下降了41%和50%（P0.05）。结论 Caveolin-1在HBV相关HCC中呈低表达，Caveolin-1的高表达抑制了HBV HBsAg的分泌。

Abstract

Objective To detect the expression of Caveolin-1 in hepatitis B virus (HBV) related hepatocellular carcinoma (HCC) and determine the correlation between Caveolin-1 and HBV infection and HCC. Methods Immunohistochemical analyses were performed to detect the expression of Caveolin-1 in 40 tissue samples of HBV related HCC and 10 samples of non-related HCC

and their corresponding 50 paracarcinoma tissues. pcDNA3.1(+) Caveolin-1 eukaryotic expression vector was constructed and the recombinant plasmid was transiently transfected into HepG2.2.15 cells. The expression of pcDNA3.1(+) Caveolin-1 was detected by Western blotting. HBsAg expression of transfected cells were determined by ELISA. Results Immunohistochemical analyses results showed a lower expression of Caveolin-1 in 95% HBV-related HCC samples compared to their corresponding paracarcinoma samples (P0.05). Western blotting indicated that the recombinant plasmids was successfully transfected into HepG2.2.15 cells and Caveolin-1 was expressed. Using ELISA analysis we observed that the levels of HBsAg in the supernatants of transfected HepG2.2.15 cells were reduced by 46.2% (48 h

P0.05) and 48.8% (72 h

P0.05) compared with the control one. And also a lower secretion of HBsAg was found in the transfected HepG2.2.15 cells (decreased by 41% for 48 h and 50% for 72 h

when compared with the non-transfected cells

P0.05). Conclusion Caveolin-1 is expressed at a low level in HBV induced HCC

while the overexpression of Caveolin-1 might down-regulate HBV HBsAg secretion.

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