Adenovirus-mediated ANT1 gene induces apoptosis of vascular smooth rat muscle cells

李燕; 卢巍; 任可; 李莹; 宋耀明

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Adenovirus-mediated ANT1 gene induces apoptosis of vascular smooth rat muscle cells

更新时间：2026-01-09

- Adenovirus-mediated ANT1 gene induces apoptosis of vascular smooth rat muscle cells
- Journal of Army Medical University Vol. 33, Issue 7, Pages: 711-714(2011)
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  CLC： R322.12;R329.28;R394.2
- Published：2011
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李燕,卢巍,任可,李莹,宋耀明.腺病毒介导过表达ANT1基因诱导大鼠血管平滑肌细胞凋亡[J].陆军军医大学学报(原第三军医大学学报),2011,33(07):711-714.

　Li Yan,Lu Wei,Ren Ke,Li Ying,Song Yaoming.Adenovirus-mediated ANT1 gene induces apoptosis of vascular smooth rat muscle cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(07):711-714.
李燕,卢巍,任可,李莹,宋耀明.腺病毒介导过表达ANT1基因诱导大鼠血管平滑肌细胞凋亡[J].陆军军医大学学报(原第三军医大学学报),2011,33(07):711-714. DOI：

　Li Yan,Lu Wei,Ren Ke,Li Ying,Song Yaoming.Adenovirus-mediated ANT1 gene induces apoptosis of vascular smooth rat muscle cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(07):711-714. DOI：

摘要

目的利用腺病毒载体转染体外培养的血管平滑肌细胞(vascular smooth muscle cells，VSMCs)，研究过表达腺嘌呤核苷酸转位酶1（adenine nucleotide translocase 1，ANT1）对大鼠VSMCs凋亡的影响。方法用携带ANT1的腺病毒载体（Ad-ANT1）或空载体腺病毒载体（Ad-GFP）感染VSMCs，采用激光共聚焦观察Hoechst33258染色后细胞凋亡情况，流式细胞术检测Annexin V标记细胞凋亡率。Western blot检测Bax和Bcl-2蛋白表达。结果激光共聚焦观察转染Ad-ANT1 后48 h细胞出现凋亡，且凋亡率[（13.40±1.14）%]与转染空载体对照组[（1.80±0.84）%]相比较差异有统计学意义(P0.01)。流式细胞术检测转染Ad-ANT1后48 h细胞凋亡率[(10.66±1.75)%]明显高于转染空载体组细胞[（3.13±0.37）%] (P0.05)。Western blot结果显示转染Ad-ANT1后Bax蛋白表达明显高于转染空载体组，Bcl-2蛋白表达2组比较无明显差异。结论腺病毒介导过表达ANT1可诱导大鼠VSMC的凋亡，其机制可能通过上调Bax实现。

Abstract

Objective To identify the effects of adenovirus mediated adenine nucleotide translocase 1 (ANT1) gene transfection on the apoptosis of rat vascular smooth muscle cells (VSMCs). Methods After adenovirus-mediated ANT1 (Ad-ANT1 ) or adenovirus (Ad-GFP) infected rat primarily cultured VSMCs

Hoechst 33258 staining for the VSMCs was observed by laser confocal microscopy for cell apoptosis. Flow cytometric analysis was used to detect the proportion of VSMCs apoptosis with Annexin V staining. The expressions of Bcl-2 and Bax were also detected by Western blot analysis. Results Laser confocal microscopy showed a progressive increase in the percentage of apoptotic VSMCs after Ad-ANT1 infection [(13.40±1.14)% in Ad-ANT1 group vs (1.80±0.84)% in Ad-GFP group

P0.01]. Flow cytometric analysis indicated a significant increase in the percentage of apoptotic VSMCs after that Ad-ANT1 infection [(10.66±1.75)% in Ad-ANT1 group vs (3.13±0.37)% in Ad-GFP group

P0.05]. The results of Western blotting indicated that the Bax expression was upregulated in VSMCs transfected with adenovirus-mediated ANT1 (Ad-ANT1 ) compared with cells of adenovirus-empty（Ad-GFP）group. Meanwhile

there is no significant difference in the Bcl-2 expression in two groups. Conclusion Overexpression of ANT1 gene may induce apoptosis of rat VSMCs through upregulating Bax expression．

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