Objective     To investigate the effect and mechanism of Mongolian drug “Tonglaga-5” on the glucose uptaking in 3T3-L1 adipocytes.     Methods     3T3-L1 adipocytes derived from 3T3-L1 preadipocytes differentiation were co-cultured with 27.27 mg/L “Tonglaga-5” for 24 h. Insulin mediated glucose uptaking in 3T3-L1 adipocytes was detected by

3

H-labeled deoxyglucose. After 3T3-L1 adipocytes coculture with “Tonglaga-5” for 48 h

the mRNA expressions of PPARγ and LXRα were determined by Real-time PCR

and their protein expressions were determined by Western blotting.    

Results     Insulin mediated glucose uptaking was increased by 1.915 times in 3T3-L1 adipocytes after Tonglaga-5 treatment when compared with normal control (P0.05). While the expression of LXRα was increase by 2.895 times at mRNA level and by 1.977 times at protein level (P0.01). But Tonglaga-5 treatment had no obvious effect on mRNA and protein expressions of PPARγ.     Conclusion     Tonglaga-5 may improve the insulin mediated glucose uptaking by up-regulating LXRα at both mRNA and protein levels in 3T3-L1 adipocytes

and then ameliorate insulin resistance.