Hypoxia-induced metallothioneins promote epithelial-mesenchymal transition of human papillary thyroid carcinoma cells

贾朝莉; 吴婷婷; 龙方懿; 刘智敏

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Hypoxia-induced metallothioneins promote epithelial-mesenchymal transition of human papillary thyroid carcinoma cells

更新时间：2026-01-09

- Hypoxia-induced metallothioneins promote epithelial-mesenchymal transition of human papillary thyroid carcinoma cells
- Journal of Army Medical University Vol. 33, Issue 7, Pages: 671-674(2011)
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  CLC： R736.1;R364.4;R730.2
- Published：2011
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贾朝莉,吴婷婷,龙方懿,刘智敏.低氧诱导金属硫蛋白对甲状腺乳头状癌上皮间质转化的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(07):671-674.

　Jia Chaoli,Wu Tingting,Long Fangyi,Liu Zhimin.Hypoxia-induced metallothioneins promote epithelial-mesenchymal transition of human papillary thyroid carcinoma cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(07):671-674.
贾朝莉,吴婷婷,龙方懿,刘智敏.低氧诱导金属硫蛋白对甲状腺乳头状癌上皮间质转化的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(07):671-674. DOI：

　Jia Chaoli,Wu Tingting,Long Fangyi,Liu Zhimin.Hypoxia-induced metallothioneins promote epithelial-mesenchymal transition of human papillary thyroid carcinoma cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(07):671-674. DOI：

摘要

目的研究低氧诱导金属硫蛋白（metallothioneins

MTs）对甲状腺乳头状癌（papillary thyroid carcinoma

PTC）上皮间质转化（epithelial-mesenchymal transition

EMT）的影响。方法以人甲状腺乳头状癌NPA细胞为研究模型，将其分为正常氧组（21%O

）、低氧组（5%O

）和MTs-siRNA干扰低氧组，Western blot、RT-PCR方法检测各组细胞MTs、上皮细胞钙粘蛋白（E-cadherin）和间质细胞波形蛋白（Vimentin）的表达，Transwell/Matrigel方法检测各组细胞侵袭转移细胞数。结果低氧可诱导NPA细胞表达MTs，并伴随有EMT标志性分子E-cadherin表达的下调和Vimentin表达的升高，侵袭细胞数由正常氧组15.6±2.8增加到40.3±5.1，迁移细胞数由25.1±5.6增加到48.7±7.6，均有统计学差异(P0.01)；MTs靶向干扰可有效抑制低氧诱导的MTs蛋白表达，同时E-cadherin表达回升，Vimentin表达下降，侵袭细胞数仅增加到25.7±4.2，迁移细胞数仅增加到35.5±5.4，与低氧组比较均有统计学差异(P0.05)。结论低氧所诱导表达的MTs对人PTC细胞发生EMT有促进作用，MTs靶向干扰可使EMT受到部分抑制。

Abstract

Objective To determine the effects of metallothioneins (MTs) induced by hypoxia on epithelial-mesenchymal transition (EMT) in human papillary thyroid carcinoma NPA cells. Methods NPA cells were divided into 3 groups

normoxia (21% O

)

hypoxia (5% O

) and hypoxia with MTs-siRNA. The expression levels of MTs

E-cadherin and Vimentin in the 3 groups of NPA cells were analyzed by Western

blotting and RT-PCR. The invasion and metastasis potential of these groups were detected by Transwell/Matrigel assay. Results Hypoxia treatment led to inducible expression of MTs

down-regulated of E-cadherin and up-regulated Vimentin at the same time

which were marker molecules of EMT in NPA cells. Meanwhile

invasive cell population of hypoxia group was increased to 40.3±5.1 from 15.6±2.8 of normoxia group (P0.01). Migrating cell population was increased to 48.7±7.6 from 25.1±5.6 of normoxia group (P0.01). However

the inducible expression of MTs was effectively inhibited by transfection of MTs-siRNA vector in NPA cells

followed by increased expression of E-cadherin and decreased expression of Vimentin. Moreover

invasive and migrating cell population of the hypoxia group with MTs-siRNA were only increased to 25.7±4.2 and 35.5±5.4(P0.05). Conclusion The expression of MTs induced by hypoxia can promote EMT in human papillary thyroid carcinoma. siRNA targeting MTs can partially inhibit EMT promoted by hypoxia.

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Department of Frigid Zone Medicine， College of High Altitude Military Medicine, School of Nursing， Army Medical University （Third Military Medical University）

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